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 quickpick
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Registered: 08/15/08
Posts: 230
Last seen: 3 years, 7 months
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 Re: LAB: Isolate and culture Lactic Acid Bactria [BIM part 1] [Re: quickpick]
#8929235 - 09/14/08 03:04 PM (3 years, 8 months ago) |
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And finally a story I LOVE!!! 
This kid is amazing, I really dig his mind set
"WCI student isolates microbe that lunches on plastic bags"
http://news.therecord.com/article/354044
Excerpt:
Quote:
Daniel, a 16-year-old Grade 11 student at Waterloo Collegiate Institute, got the idea for his project from everyday life.
"Almost every week I have to do chores and when I open the closet door, I have this avalanche of plastic bags falling on top of me," he said. "One day, I got tired of it and I wanted to know what other people are doing with these plastic bags."
The answer: not much. So he decided to do something himself.
He knew plastic does eventually degrade, and figured microorganisms must be behind it. His goal was to isolate the microorganisms that can break down plastic -- not an easy task because they don't exist in high numbers in nature.
First, he ground plastic bags into a powder. Next, he used ordinary household chemicals, yeast and tap water to create a solution that would encourage microbe growth. To that, he added the plastic powder and dirt. Then the solution sat in a shaker at 30 degrees.
After three months of upping the concentration of plastic-eating microbes, Burd filtered out the remaining plastic powder and put his bacterial culture into three flasks with strips of plastic cut from grocery bags. As a control, he also added plastic to flasks containing boiled and therefore dead bacterial culture.
Six weeks later, he weighed the strips of plastic. The control strips were the same. But the ones that had been in the live bacterial culture weighed an average of 17 per cent less.
That wasn't good enough for Burd. To identify the bacteria in his culture, he let them grow on agar plates and found he had four types of microbes. He tested those on more plastic strips and found only the second was capable of significant plastic degradation.
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quickpick
Stranger
Registered: 08/15/08
Posts: 230
Last seen: 3 years, 7 months
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Re: LAB: Isolate and culture Lactic Acid Bactria [BIM part 1] [Re: quickpick]
#8929414 - 09/14/08 03:43 PM (3 years, 8 months ago) |
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Another thought about the % of blackstrap molasses:
(reposted as an after thought to a previous post but I didn't want it to get lost as I would like comments and opinion if there are any)
An important point is that a pure culture of 1:1:10 won't provide 8% in the ferment solution. But I think that is OK as long as we use strong dilution ratio of pure culture into the ferment solution. While 1:1 is probably overkill, 1:2 or 1:3 might be wise, or a bit weaker around 1:5. In that study they are using the inherent LAB from the cod, they are not applying a pure culture of LAB. In that case a higher % of blackstrap molasses (cited as 7.5% in study) within the ferment solution is needed. But, we are using a already highly active pure culture which still has glucose that has yet to be processed and will aid when the pure culture is diluted into a ferment solution. All in all, I think we are safe suggesting 1:1:10 to make the pure culture and then 1:3, 1:5, 1:10 etc for fermenting and 1:10, 1:20, 1:50 etc, for application to bulk sub, casing, etc.
Thoughts?
Edited by quickpick (09/14/08 04:04 PM)
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Mycelio
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Registered: 06/24/08
Posts: 1,420
Loc: Berlin, Germany
Last seen: 12 hours, 34 minutes
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Re: LAB: Isolate and culture Lactic Acid Bactria [BIM part 1] [Re: quickpick]
#8929944 - 09/14/08 05:35 PM (3 years, 8 months ago) |
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How about measuring the amount of produced CO2?
If the fermentation would run in a closed bottle, leading the gases through a tube into another bottle, upside down and filled with water, you would easily see, which culture performs better. Day by day and without the need to stop the fermentation, drain and bake.
Carsten
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mycomon
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Registered: 09/07/08
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Last seen: 2 years, 5 months
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Re: LAB: Isolate and culture Lactic Acid Bactria [BIM part 1] [Re: Mycelio]
#8937743 - 09/16/08 04:43 AM (3 years, 8 months ago) |
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or possibly you could measure the # of bubbles per minute coming through the airlock, might need to give the culture a shake and wait a few minutes though
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quickpick
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Registered: 08/15/08
Posts: 230
Last seen: 3 years, 7 months
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Re: LAB: Isolate and culture Lactic Acid Bactria [BIM part 1] [Re: mycomon]
#8939033 - 09/16/08 12:18 PM (3 years, 8 months ago) |
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Hey guys,
Yea I like that idea, something visual on a daily basis. I think I may have an idea which is workable and inexpensive.
I was thinking we could use the Co2 to displace water and use the level of displaced water (in centimeters, millimeters, etc). The culture with the highest amount of displaced water should be the more aggressive fermenter. But I'm sure there are many other factors to consider but I think measuring the release of Co2 is a good 'ball park' method,
Here's my rough idea:
A large container like a 1-2 gallon ice tea pitcher. The smaller container could be a empty water bottle with the top cut off. One could hot glue the upside down water bottle to the side of the ice tea pitcher, an inch or so from the top of the pitcher. Then fill the pitcher with water, fully submersing the water bottle. Run a fish tank air hose from the bung in the culture bottle lid into the upside down water bottle. Place the culture above the pitcher as Co2 wants to sink so we might as well use that to our advantage. As the Co2 is emitted from the hose it should displace the water in the upside down water bottle. We can measure the displace by putting a light source behind the pitcher and measuring the displacement with a ruler.
Thoughts?
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Mycelio
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Registered: 06/24/08
Posts: 1,420
Loc: Berlin, Germany
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Re: LAB: Isolate and culture Lactic Acid Bactria [BIM part 1] [Re: quickpick]
#8942551 - 09/17/08 02:10 AM (3 years, 8 months ago) |
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How about using a container that is just wide enough to hold the bottle and the hose. Then you might fix the hose and the bottle could rise, when filled with CO2. Also, after a few days the bottle might be full, so you may need some hole, which you can close and open to let out CO2.
Carsten
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 cheesenoonions
??????????????
Registered: 04/01/01
Posts: 584
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Re: LAB: Isolate and culture Lactic Acid Bactria [BIM part 1] [Re: Mycelio]
#8948737 - 09/18/08 09:52 AM (3 years, 8 months ago) |
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hey sorry quick..... I was a hurricane refugee. I fled my town for a bit. I'm back though. Check your PMs
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quickpick
Stranger
Registered: 08/15/08
Posts: 230
Last seen: 3 years, 7 months
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Re: LAB: Isolate and culture Lactic Acid Bactria [BIM part 1] [Re: cheesenoonions]
#8959696 - 09/20/08 03:55 PM (3 years, 8 months ago) |
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Hey all,
Sorry for the delay of current pics.
I let the rice wash sit for 7 days. By that time it smelled sour and had a pretty thick layer on top with some sediment on the bottom. I took pictures of the jar at that point but I deleted them on accident.
I bought cheese cloth and folded it over three times. I poured the rice wash through the cheese cloth filtering out the sediment and top layer. I collected the filtered rice wash and poured 1/4 cup (1 part) into a quart jar. I then poured 2.5 cups (10 parts) organic skim milk into the jar. I screwed on the and left it a bit loose for gases to escape.
Here are a few pics of the soon to be LAB serum, aka "mother culture". The LAB serum has been sitting for 2 days at this point. The top layer has the appearance of thick curdled milk. This time there is a thin layer of what looks like "Fluff" (the white, sweet food product) on the very top. I will let the LAB serum sit for another 4-5 days then strain and refrigerate until I ferment a portion into a LAB pure culture.
I'm going to do a little multi spore side by side with a few 'vanilla' bulk sub, AEM soaked bulk sub and LAB pure culture soaked bulk sub.
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quickpick
Stranger
Registered: 08/15/08
Posts: 230
Last seen: 3 years, 7 months
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Re: LAB: Isolate and culture Lactic Acid Bactria [BIM part 1] [Re: Mycelio]
#8959704 - 09/20/08 03:58 PM (3 years, 8 months ago) |
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Hey Mycelio,
Quote:
How about using a container that is just wide enough to hold the bottle and the hose. Then you might fix the hose and the bottle could rise, when filled with CO2. Also, after a few days the bottle might be full, so you may need some hole, which you can close and open to let out CO2.
Carsten
I'm sorry I don't really see what you mean. I don't see how the bottle would rise?
Hope your well, thanks
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quickpick
Stranger
Registered: 08/15/08
Posts: 230
Last seen: 3 years, 7 months
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Re: LAB: Isolate and culture Lactic Acid Bactria [BIM part 1] [Re: cheesenoonions]
#8959717 - 09/20/08 04:00 PM (3 years, 8 months ago) |
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Hey CS,
Quote:
hey sorry quick..... I was a hurricane refugee. I fled my town for a bit. I'm back though. Check your PMs
I hope you are OK. I can wait for those items, I am sure you have more pressing matters. I hope the best for you and everyone affected.
I sent you a PM, thank you very much!
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quickpick
Stranger
Registered: 08/15/08
Posts: 230
Last seen: 3 years, 7 months
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Re: LAB: Isolate and culture Lactic Acid Bactria [BIM part 1] [Re: quickpick]
#8959860 - 09/20/08 04:31 PM (3 years, 8 months ago) |
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Oh yea,
If I had to guess as to one of the common species of lactobacillus (LAB genus) found in LAB serums around the world it would be "Lactobacillus plantarum" (L.plantarum).
L.plantarum is widespread globally and is generally a very common species of LAB found in manure and compost and is common is soil, leaf, air, etc. L.plantarum is found often, yet less commonly in the human GI tract, human vagina, etc.
L.plantarum is a well studied LAB species and is one of the main LAB species in Dr. Higa's EM (Effective Microorganisms). L.plantarum has a synergistic relationship to many organisms...
HTH
Edited by quickpick (09/20/08 04:33 PM)
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