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 Bloo Funk
Stranger
Registered: 07/17/08
Posts: 31
Last seen: 3 years, 5 months
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 Syring Cooling
#8901347 - 09/09/08 09:24 AM (3 years, 8 months ago) |
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Ok so your supposed to flame sterilize your needle before inoculation....some say to flame it and wait a second or two...some say flame it and let it cool for several minuts...some say to flame it then use an alchohol wipe to cool it and to further sterilize...some say flame it then squirt a drop or two to cool it then start innoc....So wich is it...also "IF" cubes and spores are extremeley temp sensitive (ie. incubation temp no higher than 86 ) then after flame sterilization shouldnt you let it cool for like 30-60 min....aarrgghh so confusing
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 HerbBaker
Stranger
Registered: 08/17/07
Posts: 2,390
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Re: Syring Cooling [Re: Bloo Funk]
#8901394 - 09/09/08 09:35 AM (3 years, 8 months ago) |
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86 is max ideal growing temp..
the thermal death of spores is much higher.
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Shad0w
In trouble again.
Registered: 06/08/08
Posts: 3,639
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Re: Syring Cooling [Re: Bloo Funk]
#8901398 - 09/09/08 09:35 AM (3 years, 8 months ago) |
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I flame, alcohol wipe, and inject one jar, repeat.
If the alcohol doesnt cool the needle.... the spore solution will.....
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 36,974
Loc: USA Mountain Northwest
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Re: Syring Cooling [Re: Shad0w]
#8901439 - 09/09/08 09:45 AM (3 years, 8 months ago) |
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Quote:
86 is max ideal growing temp..
Wrong. Any MYCELIUM temp above 83F slows growth considerably with P cubensis. The 86F figure is flat out wrong in TMC.
Since we know that mycelium produces heat as it grows, it's important to keep the ambient temp below 83F for best results. For Petri dishes, keep it at or below 83F, but for pf jars, keep it below 81F, and for quart jars of grains or bulk substrates, keep it below 78F to keep your mycelium in the correct temperature range.
As for a syringe needle, heat it red hot, and then use it a second or two later. It's counterproductive to wipe a red-hot needle with ANYTHING because you can't get more sterile than red-hot. The first drop or two of spore solution will cool the needle, and the rest will flow perfectly fine into your jars.
Flame until red-hot between each jar to prevent cross contamination. There is no need to do anything between holes of the same jar.
RR
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www.mushroomvideos.com
semper in excretia sumus solim profundum variat
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HerbBaker
Stranger
Registered: 08/17/07
Posts: 2,390
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good to know..now try being nice when you say it.
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veda_sticks
Cultivator
Registered: 07/29/07
Posts: 11,658
Last seen: 1 month, 2 days
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Re: Syring Cooling [Re: HerbBaker]
#8901578 - 09/09/08 10:16 AM (3 years, 8 months ago) |
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god, no matter how many times ( sometimes many times a day) you post about incubation temps the 86f misinformation still pops up.
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Roger Rabbit and Road Kill cultivation videos Starting out-This site will answer a lot of questions, great pf tek video guide
Agar's Grain LC Tek
Lipas Deli cup Agar Tek
Cakes suck for pins - click me do it right
Spice - 
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HerbBaker
Stranger
Registered: 08/17/07
Posts: 2,390
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if the ambient temp is 83, what temp is the myc at?
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Bloo Funk
Stranger
Registered: 07/17/08
Posts: 31
Last seen: 3 years, 5 months
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Re: Syring Cooling [Re: HerbBaker]
#8901615 - 09/09/08 10:24 AM (3 years, 8 months ago) |
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Your the pro RR so i wont question your technique...but damn....red hot just seem so....i dunno....hot...Hey warrior dope profile pic
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Bloo Funk
Stranger
Registered: 07/17/08
Posts: 31
Last seen: 3 years, 5 months
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Re: Syring Cooling [Re: Bloo Funk]
#8901626 - 09/09/08 10:27 AM (3 years, 8 months ago) |
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Also just for the sake of argument i leave my incubation temps at 80...I was only using 86 as a nearing thermal death temp "nearing" wat is thermal death anyway 98-109, but more importantly what is the thermal death of cube spores
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HerbBaker
Stranger
Registered: 08/17/07
Posts: 2,390
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Re: Syring Cooling [Re: Bloo Funk]
#8901704 - 09/09/08 10:46 AM (3 years, 8 months ago) |
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The thermal death points for most bacteria, molds and spores during pasteurization are as follows, 185F - to 212 F, when heated for a few seconds; 180 F for 2 minutes; 170 F for 5 minutes; 140 F for 20 minutes. Even some thermolphilic fungi and it's spores can only survive up to about 145f.
Dry mycelium may be able to withstand high temps..i know of no studies done.
As the mycelium loses water its ability to withstand cold and heat is enhanced.
This is how myc can withstand freezing temps.
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