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Invisibletripsis
biophile-misanthropist-doofer


Registered: 04/13/08
Posts: 4,138
Loc: Gondwana
Re: Puerto Rican Ganoderma Isolation! (Updated with better pics) [Re: BlimeyGrimey]
    #8732334 - 08/06/08 04:26 AM (3 months, 28 days ago)

Nice work man, kudos for the effort you've put into this project. Have you identified the species yet?


--------------------
Since we depend on an abundance of functioning ecosystems to cleanse our water, enrich our soil and manufacture the very air we breathe, biodiversity is clearly not an inheritance to be discarded carelessly - Edward O. Wilson 1992


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Invisibletripsis
biophile-misanthropist-doofer


Registered: 04/13/08
Posts: 4,138
Loc: Gondwana
Re: Puerto Rican Ganoderma Isolation! (Updated with better pics) [Re: tripsis]
    #8864154 - 09/02/08 02:15 AM (3 months, 1 day ago)

Updates?


--------------------
Since we depend on an abundance of functioning ecosystems to cleanse our water, enrich our soil and manufacture the very air we breathe, biodiversity is clearly not an inheritance to be discarded carelessly - Edward O. Wilson 1992


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OfflineBlimeyGrimey
Pyon!


Registered: 08/24/05
Posts: 1,189
Loc: Seattle, Wa
Last seen: 6 hours, 33 minutes
Re: Puerto Rican Ganoderma Isolation! (Updated with better pics) [Re: tripsis]
    #8877322 - 09/04/08 02:16 PM (2 months, 29 days ago)

The first pins I had aborted for some reason and them I had to make some room for newer projects that were over-incubating.

I've already started some new jars of spawn and this time I plan on spawning to coir/woodchips instead of plain coir.

I think the first grow aborted because of the small amount of spawn I used.

Alan has been sent a pore sample for ID. Once he gives me spore sizes and descriptions I'll look over the known Puerto Rican Ganoderma species and figure out which it really is.


--------------------
Confirmed:
Psilocybe stuntzii
Psilocybe cyanescens
Psilocybe guilartensis
Panaeolus olivaceus

Unknown:
Washington Psilocybe species
Washington Panaeolus species


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Invisibletripsis
biophile-misanthropist-doofer


Registered: 04/13/08
Posts: 4,138
Loc: Gondwana
Re: Puerto Rican Ganoderma Isolation! (Updated with better pics) [Re: BlimeyGrimey]
    #8883263 - 09/05/08 04:51 PM (2 months, 28 days ago)

Oh that's a pity, I was enjoying following this log. Staight coir for Ganoderma lucidum isn't a good substrate choice in my experience. The mycelium tore through it, but after a week or so of fruiting, 95% of the fruits spontaneously stopped growing. I don't think there are enough nutrients to support reishi in straight coir.

Good luck on your next attempt and I look forward to hearing the ID report.:thumbup:


--------------------
Since we depend on an abundance of functioning ecosystems to cleanse our water, enrich our soil and manufacture the very air we breathe, biodiversity is clearly not an inheritance to be discarded carelessly - Edward O. Wilson 1992


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OfflineMycoAu

Registered: 07/18/07
Posts: 650
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Trusted Cultivator
Re: Puerto Rican Ganoderma Isolation! (Updated with better pics) [Re: tripsis]
    #8885761 - 09/06/08 09:02 AM (2 months, 28 days ago)

Why not trying a mix with sawdust and a little nitrogen supplement (bran or something of the like).  It would be probably do much better in supporting the fruitings through harvest.  The coir would probably promote fast colonization with the wood to give the essentials for fruiting.  Adding in a few wood chips wouldn't hurt either- help to stabilize larger fruits.


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Offlinedrwatson
MysticalPhysician


Registered: 10/29/07
Posts: 500
Loc: nowhere land
Last seen: 5 days, 11 hours
Re: Puerto Rican Ganoderma Isolation! (Updated with better pics) [Re: MycoAu]
    #8886219 - 09/06/08 10:47 AM (2 months, 27 days ago)

Quote:

MycoAu said:
Why not trying a mix with sawdust and a little nitrogen supplement (bran or something of the like).  It would be probably do much better in supporting the fruitings through harvest.  The coir would probably promote fast colonization with the wood to give the essentials for fruiting.  Adding in a few wood chips wouldn't hurt either- help to stabilize larger fruits.




That sounds like a good call to me.

edit: :|


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** Emo is the disease strangling the nation **

Edited by drwatson (09/06/08 10:55 AM)


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OfflineBlimeyGrimey
Pyon!


Registered: 08/24/05
Posts: 1,189
Loc: Seattle, Wa
Last seen: 6 hours, 33 minutes
Re: Puerto Rican Ganoderma Isolation! (Updated with better pics) [Re: drwatson]
    #8902600 - 09/09/08 03:11 PM (2 months, 24 days ago)

I'll try sawdust once I get ahold of some.

I've got a bag of alder chips finally. Now I just need some sawdust and another bucket of zodoo for other projects.

Lipa has a plate of the specimen. I'm hoping he gets it to fruit at some point.


--------------------
Confirmed:
Psilocybe stuntzii
Psilocybe cyanescens
Psilocybe guilartensis
Panaeolus olivaceus

Unknown:
Washington Psilocybe species
Washington Panaeolus species


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OfflineBlimeyGrimey
Pyon!


Registered: 08/24/05
Posts: 1,189
Loc: Seattle, Wa
Last seen: 6 hours, 33 minutes
Re: Puerto Rican Ganoderma Isolation! (Updated with better pics) [Re: BlimeyGrimey]
    #8983354 - 09/25/08 09:13 AM (2 months, 9 days ago)

Small update:

Alan measured the spores of this sample for me.

The spore size are 8 - 11 by 5 - 7 micrometers and they are avocado shaped spores.


--------------------
Confirmed:
Psilocybe stuntzii
Psilocybe cyanescens
Psilocybe guilartensis
Panaeolus olivaceus

Unknown:
Washington Psilocybe species
Washington Panaeolus species


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OfflinelipaS
 User Gallery


Registered: 07/24/07
Posts: 604
Loc: Where it doesn't rain.
Last seen: 9 hours, 40 minutes
Re: Puerto Rican Ganoderma Isolation! (Updated with better pics) [Re: BlimeyGrimey]
    #8983760 - 09/25/08 11:08 AM (2 months, 8 days ago)

I just stuck this on agar last night so we will see how it goes. I will try to make up a couple of bags for comparison. Different substrates of course.


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