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 AdoreChampignons
Mycophilic One
Registered: 08/10/08
Posts: 313
Loc: Seti Alpha 5
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 Making a sterile septum(a self-sealing port for inoculation)
#8778596 - 08/15/08 05:20 PM (3 months, 18 days ago) |
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While watching how a nurse fills a syringe by first puncturing the rubber septum of a serum vial, I was amazed with how wonderfully it worked. As the nurse removed the syringe needle, the rubber sealed over the hole. There was little or no chance of contamination in or out. I was then wondering how I could make such an inoculation port on the lid of a canning jar. I got an idea on how it could be done.
Materials:
Mason jar lid
Tube of clear silicone
Sheet of mylar plastic
Hand drill
½ drill bit
1/8 drill bit
One could make a sterile septum by first drilling a half in hole in the lid near the venting hole that is stuffed with cotton. Around the half inch hole, six eighth in holes could be drilled around to act as anchoring sites. Next, place the lid on a sheet of mylar plastic then apply a layer of silicone over the ½ hole with the circle of eighth inch holes. Cover the freshly applied silicone with another sheet of mylar. Press down on the mylar so that it flattens out to about an eight inch thickness. Allow the mylar-silicone sandwich to dry for a few days. Once dry, peel off the mylar. Next, on the opposing side of the lid, apply a layer of silicone. As done previously, put a sheet of mylar over the fresh silicone. Press down on the silicone so that it flattens out to about an eighth inch in thickness. Allow the silicone to dry. Once the second layer of silicone is dry, remove the mylar sheet, and it’s ready for use. The silicone is soft enough to be pierced by any syringe needle. Now the medium can be inoculated with out even opening the jar.
The silicone that can be found in most hardware stores is an ideal medium to create the septum. It can tolerate pressure cooking quite well. It has a decomposition temperature of about 400 degree Fahrenheit. Even though silicone doesn’t stick well to metals, creating a ring of eighth inch holes around the half inch hole allows the silicone to bond through the metal and keep it from peeling off.
What do you think? Has anyone tried this before? Or, did I re-invent the wheel again?
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There is no such thing as a dumb question. There are just curious people trying to learn something new.
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 P.Menace
Contradicting the Status Quo
 
Registered: 11/17/07
Posts: 3,880
Loc: Let Me See Potato Salad!!...
Last seen: 4 days, 3 hours
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 Re: Making a sterile septum(a self-sealing port for inoculation) [Re: AdoreChampignons]
#8780240 - 08/15/08 11:56 PM (3 months, 18 days ago) |
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Red RTV silicone is the standard for this kind of thing... otherwise this is basically a reposted tek.
Not saying you stole it or anything, Great minds can truely think quite alike.
Tho what i do is usually just drill, dap RTV and smear with a salivated finger(it wont stick to your saliva) and straight on to the jar and into the PC, the heat will cook the silicone dry before you ever remove it from the PC, plus i have noticed that you get more like a true Vacutainer type of rubber out of PC'ing it while wet rather than letting it dry first.
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I just LOVE how everybody has their facts real straight...
http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace
SainT420 said: You gotta be a Man of your Word... Or you will be a Man of NO WORDS! 6 feet Under 
Prisoner # 1 said: always remember, the stupid ones never realize they're stupid... BlackMatch456,lil_demented,Dizzwizzle,djblackout
Blutjager said: "The work for payoff ratio for pf tek is like driving 500 miles to work all day for minimum wage,.  in other words ITS NOT WORTH IT "
roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.
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AdoreChampignons
Mycophilic One
Registered: 08/10/08
Posts: 313
Loc: Seti Alpha 5
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Re: Making a sterile septum(a self-sealing port for inoculation) [Re: P.Menace]
#8780439 - 08/16/08 12:37 AM (3 months, 18 days ago) |
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Ohhhh..., did I re-invent the wheel again? Ah, you use the RTV rubber? Yes, I know about that kind of silicone rubber. It's a better way to go about it but it's also kind of expensive. I went for the cheaper route. Never the less, it's good to see that others have thought along the same line of thinking.
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There is no such thing as a dumb question. There are just curious people trying to learn something new.
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FooMan
Registered: 02/02/05
Posts: 6,262
Loc: Amsterdam
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Re: Making a sterile septum(a self-sealing port for inoculation) [Re: AdoreChampignons]
#8781243 - 08/16/08 07:25 AM (3 months, 18 days ago) |
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If you want the real thing, go with a vacutainer top. themadseason.com sells them.
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Zeitgeist: Addendum<<< Watch This Entire Movie & Open Your Mind
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AdoreChampignons
Mycophilic One
Registered: 08/10/08
Posts: 313
Loc: Seti Alpha 5
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Re: Making a sterile septum(a self-sealing port for inoculation) [Re: FooMan]
#8781805 - 08/16/08 11:18 AM (3 months, 17 days ago) |
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Thank you FooMan for the web link. I checked them out. They're very reasonably priced too. Thanks a bundle.
By the way, I love the image posted under your name. It looks familiar, is it from a video game?
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There is no such thing as a dumb question. There are just curious people trying to learn something new.
Edited by AdoreChampignons (08/16/08 11:35 AM)
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lorbitherize
mush gushing
Registered: 11/24/05
Posts: 320
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Re: Making a sterile septum(a self-sealing port for inoculation) [Re: AdoreChampignons]
#8797638 - 08/19/08 06:13 PM (3 months, 14 days ago) |
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AdoreChampignons
Mycophilic One
Registered: 08/10/08
Posts: 313
Loc: Seti Alpha 5
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Re: Making a sterile septum(a self-sealing port for inoculation) [Re: lorbitherize]
#8810757 - 08/22/08 11:50 AM (3 months, 11 days ago) |
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Thank you lorbitherize for you comments. That's exactly what I was thinking of. How did it go? Did it work like a charm or were there some bugs to work out?
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There is no such thing as a dumb question. There are just curious people trying to learn something new.
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P.Menace
Contradicting the Status Quo
Registered: 11/17/07
Posts: 3,880
Loc: Let Me See Potato Salad!!...
Last seen: 4 days, 3 hours
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Re: Making a sterile septum(a self-sealing port for inoculation) [Re: AdoreChampignons]
#8811673 - 08/22/08 02:49 PM (3 months, 11 days ago) |
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It has been used/implemented for a while with no hitches... Its nothing new
--------------------
I just LOVE how everybody has their facts real straight...
http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace
SainT420 said: You gotta be a Man of your Word... Or you will be a Man of NO WORDS! 6 feet Under
Prisoner # 1 said: always remember, the stupid ones never realize they're stupid... BlackMatch456,lil_demented,Dizzwizzle,djblackout
Blutjager said: "The work for payoff ratio for pf tek is like driving 500 miles to work all day for minimum wage,. in other words ITS NOT WORTH IT "
roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.
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lorbitherize
mush gushing
Registered: 11/24/05
Posts: 320
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Re: Making a sterile septum(a self-sealing port for inoculation) [Re: AdoreChampignons]
#8812662 - 08/22/08 07:01 PM (3 months, 11 days ago) |
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As a matter of fact, it worked very well.
I've been doing it this way for quite some
time, with excellent results.
The one thing to be wary of is when flame-
sterilizing the inoculation needle, make
sure that its all the way cool before touching
it to the silicone or you'll end up having to
redo it.
By the way, it was mentioned that pressure
cooking an uncured lid will speed the process.
It may work great, but I'd have to recommend
doing it the old fashioned way and letting
them cure overnight. Who wants those nasty
fumes stinking up their cooker, not to mention
the whole room once steam begins to escape.
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AdoreChampignons
Mycophilic One
Registered: 08/10/08
Posts: 313
Loc: Seti Alpha 5
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Re: Making a sterile septum(a self-sealing port for inoculation) [Re: lorbitherize]
#8815485 - 08/23/08 12:20 PM (3 months, 10 days ago) |
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Quote:
lorbitherize said:
... Who wants those nasty
fumes stinking up their cooker, not to mention
the whole room once steam begins to escape.
Yes, I know what you mean. Sometimes a little patience goes a long way. I'm pretty sure too that those fumes and off gassing from curing silicone is quite corrosive. I read up on it earlier. I believe they actually use glacial acetic acid as the solvent for that kind of silicone.
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There is no such thing as a dumb question. There are just curious people trying to learn something new.
|
P.Menace
Contradicting the Status Quo
Registered: 11/17/07
Posts: 3,880
Loc: Let Me See Potato Salad!!...
Last seen: 4 days, 3 hours
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Re: Making a sterile septum(a self-sealing port for inoculation) [Re: lorbitherize]
#8815708 - 08/23/08 01:20 PM (3 months, 10 days ago) |
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Quote:
lorbitherize said:
By the way, it was mentioned that pressure
cooking an uncured lid will speed the process.
It may work great, but I'd have to recommend
doing it the old fashioned way and letting
them cure overnight. Who wants those nasty
fumes stinking up their cooker, not to mention
the whole room once steam begins to escape.
There is NO Fumes Try it before you Knock it.
--------------------
I just LOVE how everybody has their facts real straight...
http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace
SainT420 said: You gotta be a Man of your Word... Or you will be a Man of NO WORDS! 6 feet Under
Prisoner # 1 said: always remember, the stupid ones never realize they're stupid... BlackMatch456,lil_demented,Dizzwizzle,djblackout
Blutjager said: "The work for payoff ratio for pf tek is like driving 500 miles to work all day for minimum wage,. in other words ITS NOT WORTH IT "
roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.
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drumjam
Strangest
Registered: 01/15/06
Posts: 99
Last seen: 11 days, 14 hours
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Re: Making a sterile septum(a self-sealing port for inoculation) [Re: P.Menace]
#8846022 - 08/29/08 06:12 AM (3 months, 5 days ago) |
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Hope this works, first, sheet metal drill for cutting holes in plastic ball jar lids, helps to heat them in steamy water first. RTV, 12mm. vacutainer stoppers, and plastic ball jar lids, and lots-o-lids. Agar's LC lids w\rubber canning rings. Whatman syringe supplier, ball jar and rubber ring source, ACE Hardware, may have to order them, and vacutainer supplier. Last, cheap and easy way to filter Agar's malt\ dextrose LC. All of this worked perfectly, easily, fun, fairly fast and little mess. Thanks Agar, Roadkill, Hippychick and everyone, you sure make this fun!
If your interested here are some suppliers, I think you'll find the prices pretty good. The whatman filters are expensive but I only used them for Lc jars so not that much of a problem. That's all I Have on lids!
Edited by drumjam (08/29/08 06:29 AM)
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JaComet
Old Hand
Registered: 11/12/02
Posts: 179
Loc: Out Yonder
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Re: Making a sterile septum(a self-sealing port for inoculation) [Re: drumjam]
#8846064 - 08/29/08 06:41 AM (3 months, 5 days ago) |
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Silicone can also strengthen and patch the plugs. When new, fill the bottom half of the plug taper with a daub. Let it cure properly before use. Plugs will last longer than 3 or 4 stabs. You can also patch used plugs.
Here’s my write ups.
How To : RTV Repair of Self Healing injection plugs.
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/6040840/an/0/page/2
How to : Clean RTV injection port trick. Tyveck and Band style.
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/6014829/an/0/page/2
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drumjam
Strangest
Registered: 01/15/06
Posts: 99
Last seen: 11 days, 14 hours
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Re: Making a sterile septum(a self-sealing port for inoculation) [Re: JaComet]
#8846094 - 08/29/08 07:02 AM (3 months, 5 days ago) |
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Good to see ya again and great idea. Noced up 5 doz jars and your right I can see how they would wear due to the LC jar getting a work out. Thanks, I know I'll end up using this in the future. GL.
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FooMan
Registered: 02/02/05
Posts: 6,262
Loc: Amsterdam
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Re: Making a sterile septum(a self-sealing port for inoculation) [Re: P.Menace]
#8853198 - 08/30/08 05:29 PM (3 months, 3 days ago) |
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Quote:
P.Menace said:
Tho what i do is usually just drill, dap RTV and smear with a salivated finger(it wont stick to your saliva) and straight on to the jar and into the PC, the heat will cook the silicone dry before you ever remove it from the PC, plus i have noticed that you get more like a true Vacutainer type of rubber out of PC'ing it while wet rather than letting it dry first.
Nice tip  I'll give that a try next time I'm using RTV.
--------------------
Zeitgeist: Addendum<<< Watch This Entire Movie & Open Your Mind
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P.Menace
Contradicting the Status Quo
Registered: 11/17/07
Posts: 3,880
Loc: Let Me See Potato Salad!!...
Last seen: 4 days, 3 hours
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Re: Making a sterile septum(a self-sealing port for inoculation) [Re: FooMan]
#8853941 - 08/30/08 09:11 PM (3 months, 3 days ago) |
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Quote:
FooMan said:
Quote:
P.Menace said:
Tho what i do is usually just drill, dap RTV and smear with a salivated finger(it wont stick to your saliva) and straight on to the jar and into the PC, the heat will cook the silicone dry before you ever remove it from the PC, plus i have noticed that you get more like a true Vacutainer type of rubber out of PC'ing it while wet rather than letting it dry first.
Nice tip I'll give that a try next time I'm using RTV.
And there are no fumes like previously stated in this thread about this topic.
Think of it this way, the fumes are released for curing, whether that process happens at a higher temperature, faster, or sitting on a counter all day, the *few* fumes that are going to be released, are going to release. End of discussion
--------------------
I just LOVE how everybody has their facts real straight...
http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace
SainT420 said: You gotta be a Man of your Word... Or you will be a Man of NO WORDS! 6 feet Under
Prisoner # 1 said: always remember, the stupid ones never realize they're stupid... BlackMatch456,lil_demented,Dizzwizzle,djblackout
Blutjager said: "The work for payoff ratio for pf tek is like driving 500 miles to work all day for minimum wage,. in other words ITS NOT WORTH IT "
roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.
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