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 whiterasta
Day careobserver
Registered: 04/09/02
Posts: 1,773
Loc: Oregon
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 Culture of C.paspali for alkaloidal production, No Acid talk please!
#7018848 - 06/07/07 10:51 AM (1 year, 5 months ago) |
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Since it has been demonstrated that the ergoline profile of Claviceps paspali is very similar to MG or HBWR and has produced up to 1mg/ml in LC. These are my thoughts on strain isolation and culture for alkaloidal extraction.
A simple search will locate not only raw sclerotia,but also culture slants of C.paspali.
The raw sclerotia seems like the best bet for isolating a potent strain than chancing an ag library to have a high alkaloid strain. So basic wild print isolation techniques on agar would begin your quest for a "potent" strain. The difference with the fungi here is with a black light one can determine the alkaloid content roughly by how much it glows. So isolation sectioning should be selective for the brightest flourescing strains. Once one has a sterile strain selected for high alkaloid content it is used to inoculate a Liquid Culture using media already targeted to produce the most desired alkaloids
media for LC media maximizing Lysergic acid hydroxyethyamide production in C.paspali
Production of Simple Lysergic Acid
Derivatives and Paspalic Acid
Lysergic acid is produced by the strain Claviceps paspali. It.
synthesizes (+)-lysergic acid-a--hydroxy-ethylamide in sub-
merged culture. This substance can be converted into
(+)-lysergic acid amide, and on alkaline hydrolysis results into
(+)-lysergic acid. Lysergic acid is a key substance, in the production of ergot alkaloids and can be employed in, the chemical synthesis of their derivatives. Paspalic acid functions as the precursor of (+)-lysergic acid.
The liquid culture medium
Chemical Amount
Sorbitol/50 g
Succinic acid/36g
KH2PO4/2g
MgSO4.7H2O/0.3g
NaOH to PH 5.4
FeSO4.7H2O/10mg
ZnSO4.7H2O/1mg
Water to 1ltr.
Culture in and keep in low watt red light (40w is enough)
Alkaloid concentrations of 1mg/ml have been reported with agitated or aerated LC cultures.
Extraction of alkaloids could be simply be by adding a mild base to the culture and extracting with a suitible solvent ( remember red lights only! and as little air to the extract as possible) then salting out with tartaric acid. A rotovape would make the final drying of the extract quick and airless but kept dark and with good ventilation even at half the stated concentrations and only getting a 50% yield on the extraction. 250mg of faily pure mixed LSA's should be available per liter with the profile loaded to the more psychedelic hydroxyethylamide.
This is theory based on patent searches and research docs and I have not done this myself. I put it out as info for those who may pursue this avenue which while I am not able to pursue ,I believe hold some very important avenues for biological creation of a very special molecule.
I am VERY rusty but acetaldehyde and ethanol in a mild tartaric acid solution ( wine) could perform something very cool with the paspalic ergolines perhaps
WR
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To old for this place
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 dirtworshipper
Sitting in theheart cave
Registered: 05/12/07
Posts: 1,909
Loc: at The Guru's lotus feet
Last seen: 1 hour, 52 minutes
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 Re: Culture of C.paspali for alkaloidal production, No Acid talk please! [Re: whiterasta]
#7019032 - 06/07/07 11:52 AM (1 year, 5 months ago) |
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oh wow
thanks
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“You've got as many lives as you like, and more, even ones you don't want.” - George Harrison
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Entersandman
Thunderbird
Registered: 06/19/06
Posts: 316
Loc: Non ordinary reality
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Re: Culture of C.paspali for alkaloidal production, No Acid talk please! [Re: dirtworshipper]
#7019563 - 06/07/07 02:36 PM (1 year, 5 months ago) |
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thank you very much for this very useful information.
did you obtain a culture of Paspali??
peace
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my home is there, where human´s imagination comes to an end...
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LightShedder
Staring at Rhizos
Registered: 08/30/05
Posts: 853
Loc: Amazonia
Last seen: 32 minutes, 17 seconds
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Re: Culture of C.paspali for alkaloidal production, No Acid talk please! [Re: Entersandman]
#8850918 - 08/30/08 02:15 AM (3 months, 4 days ago) |
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I hope nobody minds me bumping this but I was very curious about this topic and thought that it'd be better to ask a question here rather than start a new topic. When you mention "basic wild print isolation" are you talking about good ole fashioned agar like the type used for various mushroom isolations? And then looking for the most florescent growth on that agar? I've always wondered about ergot strain isolation and this seems pretty relevant.
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SMILE!
YOU ARE!
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mbrown3391
Stranger
Registered: 08/22/08
Posts: 132
Last seen: 14 days, 18 hours
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Re: Culture of C.paspali for alkaloidal production, No Acid talk please! [Re: LightShedder]
#8851375 - 08/30/08 08:28 AM (3 months, 4 days ago) |
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Can you provide a link for such a website? i cannot find a single site that would sell Claviceps paspali samples.
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