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 seven
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Registered: 06/09/08
Posts: 1,122
Loc: north carolina
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 microscope isolations?
#8804494 - 08/21/08 08:24 AM (3 months, 12 days ago) |
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im curious if the technology exists to view a single mating (substrain) under a microscope and isolate with one transfer? just a thought that poped into the nugget. seems posible. im sure the lab equiptment would be costly.
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grind
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 spacel0rd
anarcho hippie
Registered: 07/21/08
Posts: 101
Last seen: 3 hours, 36 minutes
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 Re: microscope isolations? [Re: seven]
#8805057 - 08/21/08 10:39 AM (3 months, 12 days ago) |
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It is possible. I remember RogerRabbit mentioning using a microscope to identify monokaryons and transfer them. You have to look up the size of the cells you want to extract and check what microscope would do the job. Probably transferring them to a seperate plate would be the harder part. A pipette (dropping glass) should be used. There are pipettes to work on any scale. Dunno how hard it would be to use one for that specific task.
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MycoAu
Registered: 07/18/07
Posts: 650
Last seen: 13 hours, 28 minutes
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Re: microscope isolations? [Re: spacel0rd]
#8805269 - 08/21/08 11:27 AM (3 months, 12 days ago) |
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Yes, it is entirely possible. For most amateurs this is probably a bit beyond their reach until they are well-seasoned in the use of agar, culturing methods, the use of a microscope, and have developed a fair amount of dexterity (for picking out what you're after manually).
Use a magnifying glass to find a small section you're after and transfer it using a very fine tip needle (insulin syringes will work fine for this). Let it grow out about 1 cm and repeat. You should be able to do this with less work in an average laboratory/setup than trying to isolate it in one step.
Hyphal tip isolation/transfer/extraction is what you're looking for.
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check this post on down in this thread for a little discussion of it
http://www.shroomery.org/forums/showflat.php/Number/8077219#Post8077219
Edited by MycoAu (08/21/08 11:31 AM)
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seven
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Registered: 06/09/08
Posts: 1,122
Loc: north carolina
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Re: microscope isolations? [Re: MycoAu]
#8805381 - 08/21/08 11:57 AM (3 months, 12 days ago) |
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thanks for that link! fetching read. 
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grind
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 18,223
Loc: USA Mountain Northwest
Last seen: 12 hours, 28 minutes
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Re: microscope isolations? [Re: seven]
#8806207 - 08/21/08 02:53 PM (3 months, 12 days ago) |
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I can easily see individual clamp connections on a slide with the microscope, but there's no way I can hold a scalpel with my hands still enough to extract at the cellular level.
Rhizomorphic sectors on a petri dish are easily visible to the naked eye, so transferring them is still the best way for us amateur mycologists do it.
RR
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"I wouldn't want to belong to any club that would have me as a member".
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seven
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Registered: 06/09/08
Posts: 1,122
Loc: north carolina
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Re: microscope isolations? [Re: RogerRabbit]
#8806557 - 08/21/08 04:26 PM (3 months, 12 days ago) |
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so it would take some sort of high tech (human control'd) precision robotics for the manual part. lol video game mycology! thanks Spacel0rd, MycoAu, and RR . This site has some very intelligent members. I have learned so much in the past 2 months. I feel confident to start my first grow now. Didnt wanna rush anything. thanks again.
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grind
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AdoreChampignons
Mycophilic One
Registered: 08/10/08
Posts: 313
Loc: Seti Alpha 5
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Re: microscope isolations? [Re: MycoAu]
#8808563 - 08/21/08 10:21 PM (3 months, 12 days ago) |
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Quote:
MycoAu said:
Yes, it is entirely possible. For most amateurs this is probably a bit beyond their reach until they are well-seasoned in the use of agar, culturing methods...
We did it in my microbio class in college. It's really easy. One just needs to be acquainted with the streak-isolation method. First dilute the spores in a sterile solution and streak plate it. Just make sure that the agar concentration is above two percent by weight. Below two percent makes for a very soft agar that tends to tear a part when the streaking is done with the inoculation loop.
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There is no such thing as a dumb question. There are just curious people trying to learn something new.
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BrandNewbie
Captain
Registered: 05/21/08
Posts: 1,513
Loc: U.S.A.
Last seen: 12 days, 11 hours
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Quote:
AdoreChampignons said:
Quote:
MycoAu said:
Yes, it is entirely possible. For most amateurs this is probably a bit beyond their reach until they are well-seasoned in the use of agar, culturing methods...
We did it in my microbio class in college. It's really easy. One just needs to be acquainted with the streak-isolation method. First dilute the spores in a sterile solution and streak plate it. Just make sure that the agar concentration is above two percent by weight. Below two percent makes for a very soft agar that tends to tear a part when the streaking is done with the inoculation loop.
More than one way to skin a cat? Smart. 
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Help Wanted: Telepath; you know where to apply.
It's sad how whole families are torn apart by simple things, like wild dogs.
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