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 LightShedder
Familiar Friend
Registered: 08/30/05
Posts: 276
Loc: Netherlands
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 agar in 1/4 pint jars
#8629000 - 07/13/08 11:30 AM (1 month, 8 days ago) |
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I'm planning on doing agar in little mason jars rather then petri dishes. My question is, Can I put a 1/4 inch hole in the lid and stuff with polyfill for clean airflow? I am pretty sure I know the answer but just incase somebody knows something I don't please let me know.
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 FooMan
Registered: 02/02/05
Posts: 5,665
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Absolutely. Personally, I like these jars for agar work:
They're 1/2 pints, but they're also wide and really short. I drill two 1/4" holes and use postal tyvek as a filter for a clean look like so:
  
 
They're perfect for dropping pins in for cloning purposes:
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robanero
β Lib
Registered: 09/05/06
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Re: agar in 1/4 pint jars [Re: FooMan]
#8629654 - 07/13/08 03:10 PM (1 month, 8 days ago) |
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Very nice FooMan. Easier than ordering petri plates.
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310 open cap Hawaiians grown a 1 pint WBSF cake.
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LightShedder
Familiar Friend
Registered: 08/30/05
Posts: 276
Loc: Netherlands
Last seen: 1 hour, 56 minutes
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Re: agar in 1/4 pint jars [Re: robanero]
#8630046 - 07/13/08 04:50 PM (1 month, 8 days ago) |
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yeah i've got some of those. I'll use those instead. thanks for the info.
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happyday
Stranger
Registered: 06/22/04
Posts: 72
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I used to utilize baby food jars as well...with the lids on tightly(after autoclaving). I think such a small quantity of mycelium does fine without the need for additional air exchange (most species). My adult friend would eat the baby food for me (yech).
I incubated my petri dishes(or baby food jars) in ziploc bags for many years without any problems, and without reopening them until required. Now I provide them weekly air exchange(open bag and close) unless they are in long term cool(or other) storage. But you can typically get away without opening them until needed.
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 x7x_x7x
another one
Registered: 05/05/07
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Re: agar in 1/4 pint jars [Re: happyday]
#8636942 - 07/15/08 08:56 AM (1 month, 6 days ago) |
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transhumanism + primitivism=magic mushrooms
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FreeSporePrints
stronzo chilegge
Registered: 03/06/05
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Re: agar in 1/4 pint jars [Re: x7x_x7x]
#8655472 - 07/19/08 02:24 PM (1 month, 2 days ago) |
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In my opinion petri plates are better, for storing a culture is necessay a vial or a test tube.
Using jar or little jars you use more medium and you work bad.
If you don't have the money for plastic petri plate buy 20 glass petri plate one time and they last a life.
Plastic petri however are better because with the years the glass one aren't so transparent as new.
Using jars, drops of water inside the jars can be a problem, and cause bacteria contaminations or slow the growth of the mycelium.
Bye,
Fabio
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x7x_x7x
another one
Registered: 05/05/07
Posts: 604
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Quote:
FreeSporePrints said:
Using jars, drops of water inside the jars can be a problem, and cause bacteria contaminations or slow the growth of the mycelium.
drops?
i have used jars for almost one year and never got problems with contams nor slow growing.
you use less agar than petri dishes because jars diameter is smaller.
also, jars lid is an excellent barrier against external contams; petri dishes stored for a long time become contaminated easily because both parts fit loose.
other problem with petri dishes is water condensation when the agar is hot
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transhumanism + primitivism=magic mushrooms
SuctoSporeŽ
Edited by x7x_x7x (07/19/08 02:42 PM)
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trikki
cant tame the wild Menace
Registered: 06/25/08
Posts: 52
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Re: agar in 1/4 pint jars [Re: x7x_x7x]
#8674285 - 07/23/08 11:18 PM (28 days, 21 hours ago) |
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FooMan
Registered: 02/02/05
Posts: 5,665
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Re: agar in 1/4 pint jars [Re: x7x_x7x]
#8675038 - 07/24/08 02:44 AM (28 days, 17 hours ago) |
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Quote:
x7x_x7x said:
Quote:
FreeSporePrints said:
Using jars, drops of water inside the jars can be a problem, and cause bacteria contaminations or slow the growth of the mycelium.
drops?
i have used jars for almost one year and never got problems with contams nor slow growing.
you use less agar than petri dishes because jars diameter is smaller.
also, jars lid is an excellent barrier against external contams; petri dishes stored for a long time become contaminated easily because both parts fit loose.
other problem with petri dishes is water condensation when the agar is hot
^^^^^
What he said.
I have had zero problems using jars. I also use pyrex petri dishes and find that of the two, petri dishes are far more likely to have condensation than jars. Another advantage to the jars is that you can inject a spore or culture solution into them without the need for a glove box or flow hood. Yet another advantage is that they can be sterilized with the agar inside of them without worrying about the agar boiling over and making a mess. That's much easier than having to pour hot agar into plates, which again requires a glove box or flow hood to minimize contam risks.
The pros for petri dishes (IMO) are that they are cheaper (plastic ones anyway), they take up less space, they are a bit easier to store and stack and you can see through the tops of them, which makes it easier to see what kind of growth you have.
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FooMan
Registered: 02/02/05
Posts: 5,665
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Re: agar in 1/4 pint jars [Re: trikki]
#8675049 - 07/24/08 02:53 AM (28 days, 17 hours ago) |
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x7x_x7x
another one
Registered: 05/05/07
Posts: 604
Last seen: 11 seconds
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Re: agar in 1/4 pint jars [Re: FooMan]
#8675504 - 07/24/08 08:11 AM (28 days, 12 hours ago) |
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Quote:
Yet another advantage is that they can be sterilized with the agar inside of them without worrying about the agar boiling over and making a mess.
totally agree
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transhumanism + primitivism=magic mushrooms
SuctoSporeŽ
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robanero
β Lib
Registered: 09/05/06
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Re: agar in 1/4 pint jars [Re: FooMan]
#8675668 - 07/24/08 09:42 AM (28 days, 11 hours ago) |
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Quote:
FooMan said:
Quote:
x7x_x7x said:
Quote:
FreeSporePrints said:
Using jars, drops of water inside the jars can be a problem, and cause bacteria contaminations or slow the growth of the mycelium.
drops?
i have used jars for almost one year and never got problems with contams nor slow growing.
you use less agar than petri dishes because jars diameter is smaller.
also, jars lid is an excellent barrier against external contams; petri dishes stored for a long time become contaminated easily because both parts fit loose.
other problem with petri dishes is water condensation when the agar is hot
^^^^^
What he said.
I have had zero problems using jars. I also use pyrex petri dishes and find that of the two, petri dishes are far more likely to have condensation than jars. Another advantage to the jars is that you can inject a spore or culture solution into them without the need for a glove box or flow hood. Yet another advantage is that they can be sterilized with the agar inside of them without worrying about the agar boiling over and making a mess. That's much easier than having to pour hot agar into plates, which again requires a glove box or flow hood to minimize contam risks.
The pros for petri dishes (IMO) are that they are cheaper (plastic ones anyway), they take up less space, they are a bit easier to store and stack and you can see through the tops of them, which makes it easier to see what kind of growth you have.
There is a thread just for building up your post count. But why not add something useful to build it up!?!?!?
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310 open cap Hawaiians grown a 1 pint WBSF cake.
SPAWN RATIO CALCULATOR
My Little Hawaiians
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FreeSporePrints
stronzo chilegge
Registered: 03/06/05
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Loc: Rome, ITALY
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Re: agar in 1/4 pint jars [Re: robanero]
#8680230 - 07/25/08 12:28 PM (27 days, 8 hours ago) |
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i want to try the method of the jars another time and see what happens with the condensation. However the petri are really more useful than jars, speaking about space and way to work.
Fabio
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Hotnuts
old hand
Registered: 02/26/05
Posts: 2,699
Loc: Wild Blue Yawnder
Last seen: 7 hours, 7 minutes
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I have strains stored in baby food jars and they work fantastic for that, but I wouldn't recommend doing any kind of isolation work or cleaning up of cultures in a jar. Petri's are more suitable for that kind of stuff. All you have to do is keep your dishes insulated to minimize condensation.
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b3jamboree
(don't be a)Stranger
Registered: 09/11/06
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Re: agar in 1/4 pint jars [Re: Hotnuts]
#8763953 - 08/12/08 05:22 PM (9 days, 3 hours ago) |
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I had bad luck with the jars, I prefer plastic plates
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Dose Me Homie
Day Tripper
Registered: 08/12/08
Posts: 70
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Re: agar in 1/4 pint jars [Re: trikki]
#8763995 - 08/12/08 05:34 PM (9 days, 3 hours ago) |
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Quote:
trikki said:
yeah idk why that even exists. if you have nothing to add. then dont.
using jars seems like a great idea, especially because i dont know where to buy petri dishes except mycosupply. i can just go down the street n get some jars.
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Peterthinks
Dig your ownholes.
Registered: 11/10/04
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Re: agar in 1/4 pint jars [Re: FooMan]
#8769028 - 08/13/08 04:13 PM (8 days, 4 hours ago) |
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********I'm almost sold out of dishes!!*********
I got a bunch of 50 mm polypropylene dishes, they can go in the PC and be re-used.Cheaper than glass and they don't shatter if you mail cultures. PM me if you want a sleeve, 20 for 25 I pay shipping in U.S. and Canada
*****If you like my posts give em a bump, I like ratings too!******** 
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FooMan
Registered: 02/02/05
Posts: 5,665
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Re: agar in 1/4 pint jars [Re: Peterthinks]
#8781249 - 08/16/08 07:28 AM (5 days, 13 hours ago) |
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Quote:
Peterthinks said:
He used it 47 times in his last 52 posts actually....not just 9.
Probably why he's banned now. Good riddance!
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Terillius
Renaissance Man
Registered: 07/21/06
Posts: 523
Last seen: 23 hours, 46 minutes
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I just those jars with great success. I put four nail holes in the lid and then use tyvek between the lid and the jar.
Just to chime in.
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"The Prohibitionist must always be a person of no moral character;
for he cannot even conceive of the possibility of a man capable
of resisting temptation." -Aleister Crowley
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