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Offlinethepaintingaccid


Registered: 03/27/08
Posts: 223
Last seen: 10 hours, 48 minutes
Re: Puerto Rican Ganoderma Isolation! [Re: BlimeyGrimey]
    #8579636 - 06/30/08 03:46 AM (5 months, 3 days ago)

sweet progress so far. Sounds like a great isolate.


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OfflineeVenom
DRIFT on toBATTLE
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Registered: 08/25/07
Posts: 114
Loc: Florida
Last seen: 30 days, 14 hours
Re: Puerto Rican Ganoderma Isolation! [Re: thepaintingaccid]
    #8581143 - 06/30/08 05:53 PM (5 months, 2 days ago)

waiting for results!!

good luck!!!


--------------------
eVenom


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OfflineBlimeyGrimey
Pyon!


Registered: 08/24/05
Posts: 1,189
Loc: Seattle, Wa
Last seen: 5 hours, 10 minutes
Re: Puerto Rican Ganoderma Isolation! [Re: eVenom]
    #8590303 - 07/03/08 02:38 AM (5 months, 5 hours ago)

Here's a quick update pic.



--------------------
Confirmed:
Psilocybe stuntzii
Psilocybe cyanescens
Psilocybe guilartensis
Panaeolus olivaceus

Unknown:
Washington Psilocybe species
Washington Panaeolus species


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InvisibleGGreatOne234
Registered: 12/23/99
Posts: 8,670
Re: Puerto Rican Ganoderma Isolation! (Updated with better pics) [Re: BlimeyGrimey]
    #8590508 - 07/03/08 04:53 AM (5 months, 3 hours ago)

Well I guess you never really know until it fruits.
If the mycelium smells reishi you are good to go.


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OfflineBlimeyGrimey
Pyon!


Registered: 08/24/05
Posts: 1,189
Loc: Seattle, Wa
Last seen: 5 hours, 10 minutes
Re: Puerto Rican Ganoderma Isolation! (Updated with better pics) [Re: GGreatOne234]
    #8590556 - 07/03/08 05:18 AM (5 months, 2 hours ago)

I'm not sure what resihi mycelium smells like. I'm pretty sure its clean mycelium. I'm just doubting the way I decided to fruit them. Either I have alot of small ones starting to form or I'm gonna end up with a reishi blob.

Got any pics of resihi pins?


--------------------
Confirmed:
Psilocybe stuntzii
Psilocybe cyanescens
Psilocybe guilartensis
Panaeolus olivaceus

Unknown:
Washington Psilocybe species
Washington Panaeolus species


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OfflineMycoAu

Registered: 07/18/07
Posts: 650
Last seen: 13 hours, 22 minutes
Trusted Cultivator
Re: Puerto Rican Ganoderma Isolation! (Updated with better pics) [Re: BlimeyGrimey]
    #8592203 - 07/03/08 04:27 PM (4 months, 30 days ago)

You might ask Lipa about the pins.  Lipa's got some growing at the moment and has been taking a lot of pictures along the way.


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OfflinelipaS
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Registered: 07/24/07
Posts: 604
Loc: Where it doesn't rain.
Last seen: 8 hours, 17 minutes
Re: Puerto Rican Ganoderma Isolation! (Updated with better pics) [Re: MycoAu]
    #8592792 - 07/03/08 07:23 PM (4 months, 30 days ago)

I didn't take any while they were really small. This is about 2 days after I noticed them.



Good luck man! Can't wait to see the results!!:thumbup:


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OfflineBlimeyGrimey
Pyon!


Registered: 08/24/05
Posts: 1,189
Loc: Seattle, Wa
Last seen: 5 hours, 10 minutes
Re: Puerto Rican Ganoderma Isolation! (Updated with better pics) [Re: lipa]
    #8637353 - 07/15/08 11:32 AM (4 months, 18 days ago)

Quick Update.
Not much has changed.



There are some weird spots. Seems to be small drops of metabolites encased in myc web balls. You can see a few in the picture if you look for them.


--------------------
Confirmed:
Psilocybe stuntzii
Psilocybe cyanescens
Psilocybe guilartensis
Panaeolus olivaceus

Unknown:
Washington Psilocybe species
Washington Panaeolus species


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OfflineMycoAu

Registered: 07/18/07
Posts: 650
Last seen: 13 hours, 22 minutes
Trusted Cultivator
Re: Puerto Rican Ganoderma Isolation! (Updated with better pics) [Re: BlimeyGrimey]
    #8637903 - 07/15/08 01:27 PM (4 months, 18 days ago)

I should have my camera back tomorrow.  Then I'll be able to take pictures of my reishi.  I'll post when I can.  (just barely beginning to form fruits)


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OfflineThe
Professional Misanthrope
Male


Registered: 07/09/08
Posts: 132
Last seen: 2 months, 15 days
Re: Puerto Rican Ganoderma Isolation! (Updated with better pics) [Re: MycoAu]
    #8644324 - 07/16/08 07:56 PM (4 months, 17 days ago)

wow,this is the most interesting thing I've scene all week.

I wonder if the mushrooms will take on their distinct look in 100% humidity.


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Offlinetrikki
cant tame the wild Menace
Male


Registered: 06/25/08
Posts: 53
Last seen: 6 days, 16 hours
Re: Puerto Rican Ganoderma Isolation! [Re: BlimeyGrimey]
    #8674323 - 07/23/08 11:25 PM (4 months, 10 days ago)

:nothingtoadd:


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OfflineFreeSporePrints
stronzo chilegge
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Registered: 03/06/05
Posts: 910
Loc: Rome, ITALY
Last seen: 2 days, 6 hours
Re: Puerto Rican Ganoderma Isolation! (Updated with better pics) [Re: BlimeyGrimey]
    #8680239 - 07/25/08 12:31 PM (4 months, 8 days ago)

Haven't you added any antibiotic to the agar?

Thanks,
Fabio


--------------------


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OfflineBlimeyGrimey
Pyon!


Registered: 08/24/05
Posts: 1,189
Loc: Seattle, Wa
Last seen: 5 hours, 10 minutes
Re: Puerto Rican Ganoderma Isolation! (Updated with better pics) [Re: FreeSporePrints]
    #8688745 - 07/27/08 03:21 PM (4 months, 6 days ago)

No antibiotic was used and I still got a clean culture the very first try. I simply used a piece of pore tissue from the center of the conk and wiped it across agar. Only one spot of germination ever appeared. I then transferred a piece of the new growth to a new plate.

The cake has a small spot which I think is the beginning of a fruit. I'll post a pic(s) in a bit.

EDIT:

Here's the updated pic.



The red arrows point to what seem to be fruit formations. Yellowish overall but the center is darkening up.

The green arrows point to myc piss drops which are being covered with mycelium. This is how the "fruits" looked a week ago. They were drops that slowly got covered with mycelium then started yellowing, then darkening.

The blue arrow points to a myc piss drop that is no yet covered in mycelium.


--------------------
Confirmed:
Psilocybe stuntzii
Psilocybe cyanescens
Psilocybe guilartensis
Panaeolus olivaceus

Unknown:
Washington Psilocybe species
Washington Panaeolus species

Edited by BlimeyGrimey (07/27/08 05:04 PM)


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OfflineBlimeyGrimey
Pyon!


Registered: 08/24/05
Posts: 1,189
Loc: Seattle, Wa
Last seen: 5 hours, 10 minutes
Re: Puerto Rican Ganoderma Isolation! (Updated with better pics) [Re: BlimeyGrimey]
    #8715058 - 08/02/08 05:05 AM (4 months, 1 day ago)

I left some of the barley spawn in the bottom of the jar. About 2 tablespoons worth. This is what it looks like after 3 weeks.



--------------------
Confirmed:
Psilocybe stuntzii
Psilocybe cyanescens
Psilocybe guilartensis
Panaeolus olivaceus

Unknown:
Washington Psilocybe species
Washington Panaeolus species


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Invisiblerugergirl79
lεṡвïαṉ εχτɾøḋïṉ^
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Registered: 03/27/08
Posts: 1,772
Loc: cardboard box
Re: Puerto Rican Ganoderma Isolation! (Updated with better pics) [Re: BlimeyGrimey]
    #8715082 - 08/02/08 05:31 AM (4 months, 1 day ago)

you have some interesting stuff going on in this thread......congrats on your luck with the no contams...I am going to keep track on your success with this.....very interesting stuff...thanks for sharing blimey...kat  :rockon:


--------------------
"I hate to advocate drugs, alcohol, violence or insanity to anyone, but they've always worked for me."
Hunter S. Thompson
                                                                 
                                                                Worked for these guys as well!


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OfflineBlimeyGrimey
Pyon!


Registered: 08/24/05
Posts: 1,189
Loc: Seattle, Wa
Last seen: 5 hours, 10 minutes
Re: Puerto Rican Ganoderma Isolation! (Updated with better pics) [Re: rugergirl79]
    #8715092 - 08/02/08 05:51 AM (4 months, 1 day ago)

I now have a definite pin starting. The previous spots I thought were fruitbodies don't seem to be growing. I noticed a growth near the edge of the container that started to stretch slightly and is turning a bright yellow color. Starting to get that shine to it too. I'll take a pic of it in a few days once its big enough for a decent photo.


--------------------
Confirmed:
Psilocybe stuntzii
Psilocybe cyanescens
Psilocybe guilartensis
Panaeolus olivaceus

Unknown:
Washington Psilocybe species
Washington Panaeolus species


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Invisiblerugergirl79
lεṡвïαṉ εχτɾøḋïṉ^
 User Gallery


Registered: 03/27/08
Posts: 1,772
Loc: cardboard box
Re: Puerto Rican Ganoderma Isolation! (Updated with better pics) [Re: BlimeyGrimey]
    #8715097 - 08/02/08 05:57 AM (4 months, 1 day ago)

Cool...i will be on the lookout for an update pic...kat


--------------------
"I hate to advocate drugs, alcohol, violence or insanity to anyone, but they've always worked for me."
Hunter S. Thompson
                                                                 
                                                                Worked for these guys as well!


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OfflineBlimeyGrimey
Pyon!


Registered: 08/24/05
Posts: 1,189
Loc: Seattle, Wa
Last seen: 5 hours, 10 minutes
Re: Puerto Rican Ganoderma Isolation! (Updated with better pics) [Re: rugergirl79]
    #8727558 - 08/05/08 04:03 AM (3 months, 29 days ago)

Here's a new updated pic. I think the bright yellow spots might be small fruits starting.



--------------------
Confirmed:
Psilocybe stuntzii
Psilocybe cyanescens
Psilocybe guilartensis
Panaeolus olivaceus

Unknown:
Washington Psilocybe species
Washington Panaeolus species


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Offlineinstantdeath
Stranger
Registered: 08/02/08
Posts: 69
Last seen: 3 months, 28 days
Re: Puerto Rican Ganoderma Isolation! (Updated with better pics) [Re: BlimeyGrimey]
    #8728118 - 08/05/08 09:34 AM (3 months, 28 days ago)

Interesting stuff!!  Thanks for sharing


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OfflineMycoAu

Registered: 07/18/07
Posts: 650
Last seen: 13 hours, 22 minutes
Trusted Cultivator
Re: Puerto Rican Ganoderma Isolation! (Updated with better pics) [Re: instantdeath]
    #8728692 - 08/05/08 12:01 PM (3 months, 28 days ago)

Blimey, those are indeed fruits beginning to grow.  You should see elongation and broadening within the next few days to a week (to the point of seeing a more recognizable "reishi" shape).


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Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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