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 P.Menace
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Registered: 11/17/07
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 Salicylic Acid Agar
#8565295 - 06/26/08 02:01 AM (5 months, 7 days ago) |
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So I just received my shipment of Salicylic Acid... I was sitting here running thru the numbers of what needs to be added, and there isnt much info on this particular agar additive...
So I was looking at the ratio's added of other products to a Quart of agar mix...
Gentomyicin Sulfate is added to agar by 1/20th or 0.05 Grams to 1 quart of your favorite agar mix, to create an antibacterial agar.
Some have used Hydrogen Peroxide in ratios of up to 4% or 40ml to 1 Quart of Pre-sterilized agar mix... H202 will break down in the PC... to create an Antibacterial Agar.
Snake Venom has been used in Agar to attempt to create "Hybrid" and/or Cross Species, some interesting experiments have been done, and interesting hybrids/cross-species/interspecies-hybrids created using this method... But there is no info on how much should be added to the agar mix.
Salicylic Acid has been used in the medical field in concentrations up to 35% and over the counter in concentrations up to 17% for the use of Wart removal and a short list of other things.
So after looking at all of this, plus basic sugar ratios and whatnot used in agar, I believe that this additive would work well at a concentration level of up to 1/4 or 0.25 grams Per Quart, and still be useful to my needs, tho i may begin at a lesser concentration and make some observations under the microscope during some simple experiments to try and find the most useful concentration, without harming the mycelium's growth, yet still allowing for the deconstruction of the cell walls...
Maybe someone with more experience than I can help me out here, because my figures could be way off...
FF or Anno Please Chime IN!
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I just LOVE how everybody has their facts real straight...
http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace
SainT420 said: You gotta be a Man of your Word... Or you will be a Man of NO WORDS! 6 feet Under 
Prisoner # 1 said: always remember, the stupid ones never realize they're stupid... BlackMatch456,lil_demented,Dizzwizzle,djblackout
Blutjager said: "The work for payoff ratio for pf tek is like driving 500 miles to work all day for minimum wage,.  in other words ITS NOT WORTH IT "
roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.
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P.Menace
Contradicting the Status Quo
Registered: 11/17/07
Posts: 3,880
Loc: Let Me See Potato Salad!!...
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 Re: Salicylic Acid Agar [Re: P.Menace]
#8565390 - 06/26/08 03:01 AM (5 months, 7 days ago) |
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Salicylic Acid is also used for the treatment of Excema, Psoriasis, and Acne, In concentrations ranging from 1/16th of a teaspoon (???weight???) to 20% of the total contents... so i dont think that 20% or 8 grams per quart of agar would be right...
Now im gonna have to break out the scale and weigh 1/16th of a teaspoon of salicylic acid... Any ideas how much 1/16th of a teaspoon looks like??? cause that sure is one whacked ass method of measurement!
Weigh a Level Teaspoon and divide by 16 i guess...
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I just LOVE how everybody has their facts real straight...
http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace
SainT420 said: You gotta be a Man of your Word... Or you will be a Man of NO WORDS! 6 feet Under
Prisoner # 1 said: always remember, the stupid ones never realize they're stupid... BlackMatch456,lil_demented,Dizzwizzle,djblackout
Blutjager said: "The work for payoff ratio for pf tek is like driving 500 miles to work all day for minimum wage,. in other words ITS NOT WORTH IT "
roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.
Edited by P.Menace (06/26/08 03:10 AM)
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P.Menace
Contradicting the Status Quo
Registered: 11/17/07
Posts: 3,880
Loc: Let Me See Potato Salad!!...
Last seen: 4 days, 3 hours
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Re: Salicylic Acid Agar [Re: P.Menace]
#8567151 - 06/26/08 02:50 PM (5 months, 6 days ago) |
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Really? No one Knows?
Experiment time!!!
--------------------
I just LOVE how everybody has their facts real straight...
http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace
SainT420 said: You gotta be a Man of your Word... Or you will be a Man of NO WORDS! 6 feet Under
Prisoner # 1 said: always remember, the stupid ones never realize they're stupid... BlackMatch456,lil_demented,Dizzwizzle,djblackout
Blutjager said: "The work for payoff ratio for pf tek is like driving 500 miles to work all day for minimum wage,. in other words ITS NOT WORTH IT "
roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 18,223
Loc: USA Mountain Northwest
Last seen: 12 hours, 21 minutes
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Re: Salicylic Acid Agar [Re: P.Menace]
#8567276 - 06/26/08 03:25 PM (5 months, 6 days ago) |
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I have no experience with it, so unless someone else does, you'll have to guess at the amount to use.
If you're trying to cross cube strains, it might help with dikaryotic/dikaryotic crosses, but won't be necessary if you're wishing to cross monokaryons from different strains. They should readily cross if they're compatible.
RR
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www.mushroomvideos.com
"I wouldn't want to belong to any club that would have me as a member".
Mark Twain, Woody Allen, Groucho Marx, and anyone else who wishes to claim credit for the quote.
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P.Menace
Contradicting the Status Quo
Registered: 11/17/07
Posts: 3,880
Loc: Let Me See Potato Salad!!...
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Yeah i was planning to use it in regards to Dikaryotic crosses of cube strains.
I will bust out some experimentation and log my results... not a big deal, just a small side project
--------------------
I just LOVE how everybody has their facts real straight...
http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace
SainT420 said: You gotta be a Man of your Word... Or you will be a Man of NO WORDS! 6 feet Under
Prisoner # 1 said: always remember, the stupid ones never realize they're stupid... BlackMatch456,lil_demented,Dizzwizzle,djblackout
Blutjager said: "The work for payoff ratio for pf tek is like driving 500 miles to work all day for minimum wage,. in other words ITS NOT WORTH IT "
roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.
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MycoAu
Registered: 07/18/07
Posts: 650
Last seen: 13 hours, 22 minutes
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Re: Salicylic Acid Agar [Re: P.Menace]
#8568837 - 06/26/08 09:17 PM (5 months, 6 days ago) |
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If I remember correctly, 1 tsp = 5 mL. If you're talking about water, that's also 1 gram. So, basic assumption is 5 grams/tsp. So, 5grams / 16 (or 1/16th of a tsp) is 0.3125 grams (or 312.5 mg). You do a search on google and I'm sure you'll eventually be able to find a density for salicylic acid to do a weight correction to make a better estimate/assumption of weight. But that should at least give you so place to start! Good luck and keep us posted!
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P.Menace
Contradicting the Status Quo
Registered: 11/17/07
Posts: 3,880
Loc: Let Me See Potato Salad!!...
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Re: Salicylic Acid Agar [Re: MycoAu]
#8568871 - 06/26/08 09:25 PM (5 months, 6 days ago) |
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well thankyou for that... but i already weighed it and you are so very close to correct that those are actually the numbers that i am basing my estimates on...
I am going to try 2 ratios... 1 @ 312.5mg and another @ 625mg
Im sure either would work to do what i want, however at higher concentrations it may be dangerous to the Mycelium it self... so like i said...
To the experiments!
--------------------
I just LOVE how everybody has their facts real straight...
http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace
SainT420 said: You gotta be a Man of your Word... Or you will be a Man of NO WORDS! 6 feet Under
Prisoner # 1 said: always remember, the stupid ones never realize they're stupid... BlackMatch456,lil_demented,Dizzwizzle,djblackout
Blutjager said: "The work for payoff ratio for pf tek is like driving 500 miles to work all day for minimum wage,. in other words ITS NOT WORTH IT "
roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.
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MycoAu
Registered: 07/18/07
Posts: 650
Last seen: 13 hours, 22 minutes
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Re: Salicylic Acid Agar [Re: P.Menace]
#8573895 - 06/28/08 10:07 AM (5 months, 4 days ago) |
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BlimeyGrimey
Pyon!
Registered: 08/24/05
Posts: 1,189
Loc: Seattle, Wa
Last seen: 5 hours, 10 minutes
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Re: Salicylic Acid Agar [Re: MycoAu]
#8574019 - 06/28/08 11:09 AM (5 months, 4 days ago) |
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Hehe, I was actually gonna post it here first but I didn't want to hijack his thread so I started my own.
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Confirmed:
Psilocybe stuntzii
Psilocybe cyanescens
Psilocybe guilartensis
Panaeolus olivaceus
Unknown:
Washington Psilocybe species
Washington Panaeolus species
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MycoAu
Registered: 07/18/07
Posts: 650
Last seen: 13 hours, 22 minutes
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it's nice to keep all pertinent information on a topic cross-linked or in one thread. It's not like we have to talk about your topic if you include here. Discussion of his method will continue if there is still something to talk about. If not, that has nothing to do with you including more information. (Plus, you may end up saving him a ton of work if it's a working, proven method.)
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P.Menace
Contradicting the Status Quo
Registered: 11/17/07
Posts: 3,880
Loc: Let Me See Potato Salad!!...
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Re: Salicylic Acid Agar [Re: MycoAu]
#8574596 - 06/28/08 03:08 PM (5 months, 4 days ago) |
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Interesting!
Nictonic Acid (niacin) can be used... i may have some other experiments to run
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I just LOVE how everybody has their facts real straight...
http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace
SainT420 said: You gotta be a Man of your Word... Or you will be a Man of NO WORDS! 6 feet Under
Prisoner # 1 said: always remember, the stupid ones never realize they're stupid... BlackMatch456,lil_demented,Dizzwizzle,djblackout
Blutjager said: "The work for payoff ratio for pf tek is like driving 500 miles to work all day for minimum wage,. in other words ITS NOT WORTH IT "
roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.
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BlimeyGrimey
Pyon!
Registered: 08/24/05
Posts: 1,189
Loc: Seattle, Wa
Last seen: 5 hours, 10 minutes
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Re: Salicylic Acid Agar [Re: P.Menace]
#8576117 - 06/29/08 12:36 AM (5 months, 4 days ago) |
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The best thing about that niacin pdf is that it gives you a recipe for the niacin agar. Takes a lot of the guesswork out of the process.
--------------------
Confirmed:
Psilocybe stuntzii
Psilocybe cyanescens
Psilocybe guilartensis
Panaeolus olivaceus
Unknown:
Washington Psilocybe species
Washington Panaeolus species
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P.Menace
Contradicting the Status Quo
Registered: 11/17/07
Posts: 3,880
Loc: Let Me See Potato Salad!!...
Last seen: 4 days, 3 hours
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Yeah... and Nictonic Acid is readily available almost anywhere
--------------------
I just LOVE how everybody has their facts real straight...
http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace
SainT420 said: You gotta be a Man of your Word... Or you will be a Man of NO WORDS! 6 feet Under
Prisoner # 1 said: always remember, the stupid ones never realize they're stupid... BlackMatch456,lil_demented,Dizzwizzle,djblackout
Blutjager said: "The work for payoff ratio for pf tek is like driving 500 miles to work all day for minimum wage,. in other words ITS NOT WORTH IT "
roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.
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 fastfred
Registered: 05/17/04
Posts: 4,366
Loc: Dark side of the moon
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Re: Salicylic Acid Agar [Re: P.Menace]
#8644926 - 07/16/08 10:27 PM (4 months, 17 days ago) |
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I would suggest starting with 2-5%. That concentration should cause some effects without being overly toxic. I've never used salicylic acid in agar before, but your best bet would be to do some experiments and find the highest concentration that still allow acceptable growth.
Be sure to post your results as this is an interesting topic.
-FF
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P.Menace
Contradicting the Status Quo
Registered: 11/17/07
Posts: 3,880
Loc: Let Me See Potato Salad!!...
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Re: Salicylic Acid Agar [Re: fastfred]
#8645136 - 07/16/08 11:12 PM (4 months, 17 days ago) |
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According to some online calculators that equals out to be somewhere in the 2-5 grams per quart for a 2-5% concentration... does that seem right to you?
FF you can be a big help sometimes
--------------------
I just LOVE how everybody has their facts real straight...
http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace
SainT420 said: You gotta be a Man of your Word... Or you will be a Man of NO WORDS! 6 feet Under
Prisoner # 1 said: always remember, the stupid ones never realize they're stupid... BlackMatch456,lil_demented,Dizzwizzle,djblackout
Blutjager said: "The work for payoff ratio for pf tek is like driving 500 miles to work all day for minimum wage,. in other words ITS NOT WORTH IT "
roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.
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trikki
cant tame the wild Menace
Registered: 06/25/08
Posts: 53
Last seen: 6 days, 16 hours
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Re: Salicylic Acid Agar [Re: P.Menace]
#8674317 - 07/23/08 11:24 PM (4 months, 10 days ago) |
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MycoAu
Registered: 07/18/07
Posts: 650
Last seen: 13 hours, 22 minutes
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Re: Salicylic Acid Agar [Re: P.Menace]
#8680403 - 07/25/08 01:23 PM (4 months, 8 days ago) |
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try 2-5 grams per 100 ml of water when making up your agar
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P.Menace
Contradicting the Status Quo
Registered: 11/17/07
Posts: 3,880
Loc: Let Me See Potato Salad!!...
Last seen: 4 days, 3 hours
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Re: Salicylic Acid Agar [Re: MycoAu]
#8680416 - 07/25/08 01:27 PM (4 months, 8 days ago) |
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that would be a 20-50% solution.
I found an online calculator that figures these ratios of dry ingredients to water.
--------------------
I just LOVE how everybody has their facts real straight...
http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace
SainT420 said: You gotta be a Man of your Word... Or you will be a Man of NO WORDS! 6 feet Under
Prisoner # 1 said: always remember, the stupid ones never realize they're stupid... BlackMatch456,lil_demented,Dizzwizzle,djblackout
Blutjager said: "The work for payoff ratio for pf tek is like driving 500 miles to work all day for minimum wage,. in other words ITS NOT WORTH IT "
roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.
|
P.Menace
Contradicting the Status Quo
Registered: 11/17/07
Posts: 3,880
Loc: Let Me See Potato Salad!!...
Last seen: 4 days, 3 hours
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Re: Salicylic Acid Agar [Re: P.Menace]
#8719882 - 08/03/08 01:49 PM (3 months, 30 days ago) |
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Ok.
2 experiments about to go underway...
1a. Salicylic acid concentration of 2.5%... Acid will be added first, to look for degradation during Sterilization Process.
1b. Salicylic acid concentration of 5%... Acid will be added first, to look for degradation during Sterilization Process.
2a. Salicylic acid concentration of 2.5%... Acid will be added after Sterilization in front of Flowhood
2a. Salicylic acid concentration of 5%... Acid will be added after Sterilization in front of Flowhood
Ph will be tested in agar solution of 2.5% and 5% before and after Sterilization, while also testing base Ph of agar solution without the Salicylic Acid.
I will be pouring them into small 60mmX15mm petri dishes, allow to cool for 24 hours in front of flowhood before inoculation occurs.
If I seem to be missing anything, or you have a particular method you would like to see used/tested let me know.
FastFred, I could always use your approval. Your the Agar guy
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I just LOVE how everybody has their facts real straight...
http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace
SainT420 said: You gotta be a Man of your Word... Or you will be a Man of NO WORDS! 6 feet Under
Prisoner # 1 said: always remember, the stupid ones never realize they're stupid... BlackMatch456,lil_demented,Dizzwizzle,djblackout
Blutjager said: "The work for payoff ratio for pf tek is like driving 500 miles to work all day for minimum wage,. in other words ITS NOT WORTH IT "
roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.
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Psuper
W-W-Wyzzerdd
Registered: 02/12/08
Posts: 1,032
Loc: PNW (where else?)
Last seen: 10 hours, 37 minutes
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Re: Salicylic Acid Agar [Re: P.Menace]
#8721867 - 08/03/08 10:28 PM (3 months, 30 days ago) |
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Interesting thread P. 
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M.S.---> rye berries---> coco-coir, coffee grounds, vermiculite, gypsum---> 6quart trays, no casing layer
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