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 agmotes165
I only wish i was this good!
 
Registered: 12/30/07
Posts: 377
Loc: Southeast, US
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 Oxygenation of Liquid Culture by Hydrogen peroxide breakdown
#8660261 - 07/20/08 06:57 PM (4 months, 13 days ago) |
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Ok, so Hydrogen peroxide is said to breakdown rapidly in the presence of light, So why could you not prepare the LC using Hydrogen peroxide, then after PCing just set the jars out in full sunlight for a full day or so, wouldnt the Hydrogen Peroxide breakdown into water and Oxygen, thereby adding oxygen to the LC? Also, since the hydrogen peroxide kills spores and such, i dont think you would necessarily have to PC it, you could just boil it for 30 minutes or so...and since the hydrogen peroxide would breakdown...could you use spore syringes as well? I'm kindof new at this so if you could please excuse my naivety and tell me why this couldn't work or if it could work that would be awesome. Thanks in advance...
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all things are relative, its either a tv or an x-ray tube depending on where ur standing...
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waixingren
I am the FrizzleFry
Registered: 03/14/05
Posts: 1,660
Loc: South Florida
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 Re: Oxygenation of Liquid Culture by Hydrogen peroxide breakdown [Re: agmotes165]
#8660575 - 07/20/08 08:30 PM (4 months, 13 days ago) |
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the peroxide would break down in the PC, you would have to add it after the lc had cooled down.
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"The real secret of magic is that the world is made of words. And that if you know the words that the world is made of, you can make of it whatever you wish. "
-T.M.
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 ohmatic
searcher
Registered: 02/28/04
Posts: 5,040
Loc: europe
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Re: Oxygenation of Liquid Culture by Hydrogen peroxide breakdown [Re: waixingren]
#8661790 - 07/21/08 03:50 AM (4 months, 13 days ago) |
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Quote:
waixingren said:
the peroxide would break down in the PC, you would have to add it after the lc had cooled down.
word it wouldnt survive the pc
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FGL
Stranger
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Re: Oxygenation of Liquid Culture by Hydrogen peroxide breakdown [Re: ohmatic]
#8662589 - 07/21/08 10:48 AM (4 months, 12 days ago) |
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forget about spores LC and forget about adding peroxide (it will kill the spores or will harm the mycelium)
this is the real way to obtain clean liquid mycelium:
http://www.shroomery.org/forums/showflat.php/Number/6817701#6817701
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Make your own Magnetic Stirrer for L.C ( Several designes Links)
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agmotes165
I only wish i was this good!
Registered: 12/30/07
Posts: 377
Loc: Southeast, US
Last seen: 31 minutes
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Re: Oxygenation of Liquid Culture by Hydrogen peroxide breakdown [Re: FGL]
#8663638 - 07/21/08 03:15 PM (4 months, 12 days ago) |
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ok but what if you are just boiling the LC, would the hydrogen peroxide help keep everything sterile until it breaks down in light, then provide enough oxygen to promote healthy spore germination?
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all things are relative, its either a tv or an x-ray tube depending on where ur standing...
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lipa
Registered: 07/24/07
Posts: 604
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Re: Oxygenation of Liquid Culture by Hydrogen peroxide breakdown [Re: agmotes165]
#8663764 - 07/21/08 04:01 PM (4 months, 12 days ago) |
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Mycelium doesn't need the extra oxygen. It needs aeration more than it needs the oxygen. Keeping the lc moving is more important and provides a better mixture of food and air. And by air I don't just mean oxygen. Its like putting a fan in a FC. If the fan is on inside, more of what the mushrooms need passes by them more often. Greater yields can be had from just mixing everything evenly. You can experiment with this. Just make one lc, shake it once, and let it sit. Make another shake it 3 times a day and see which does better. It's as simple as that. You are not going to get better yields just because the LC is more oxygenated. LC is just to get mycelium/liquid mixture for inoculation. True that LCs that are not shaken produce a less vigorous result, but that will soon change as soon as you put them into jars of grain or medium.
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agmotes165
I only wish i was this good!
Registered: 12/30/07
Posts: 377
Loc: Southeast, US
Last seen: 31 minutes
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Re: Oxygenation of Liquid Culture by Hydrogen peroxide breakdown [Re: lipa]
#8668181 - 07/22/08 03:08 PM (4 months, 11 days ago) |
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Awesome...Thanks guys and gals for answering my question, I learned more than i expected about LC. You Guys are awesome
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all things are relative, its either a tv or an x-ray tube depending on where ur standing...
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trikki
cant tame the wild Menace
Registered: 06/25/08
Posts: 53
Last seen: 6 days, 16 hours
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Re: Oxygenation of Liquid Culture by Hydrogen peroxide breakdown [Re: agmotes165]
#8674274 - 07/23/08 11:16 PM (4 months, 10 days ago) |
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