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Offlinemetalhead
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cold sterilization
    #8663613 - 07/21/08 03:08 PM (4 months, 12 days ago)

i asked this in the cultivation forum and not many hits so here it goes..can you cold sterilize lc and what not using uv lighting like they do using them to sterilize h2o for human consumption?


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OfflineMVP
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Re: cold sterilization [Re: metalhead]
    #8664524 - 07/21/08 07:22 PM (4 months, 12 days ago)

no, no, and no.


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OfflineHazeyRoms
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Re: cold sterilization [Re: MVP]
    #8666390 - 07/22/08 02:12 AM (4 months, 12 days ago)

Why, Why and Why??? Be specific. Don't tell me you are one of those people who whoop there kids ass and not tell them what you did wrong, are you??


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Onlinedstark
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Re: cold sterilization [Re: HazeyRoms]
    #8666398 - 07/22/08 02:15 AM (4 months, 12 days ago)

Because this question was covered many times,
Cold only slows growth.

If you have a amchine which does -274c` which is comjpletely freeze when the atoms stop moving then you might :wink:


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OfflineTsukishade
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Re: cold sterilization [Re: metalhead]
    #8667046 - 07/22/08 09:50 AM (4 months, 11 days ago)



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Offlineworowa
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Re: cold sterilization [Re: Tsukishade]
    #8668533 - 07/22/08 05:39 PM (4 months, 11 days ago)

Try it, then share your results.


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Offlinetrikki
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Re: cold sterilization [Re: worowa]
    #8674257 - 07/23/08 11:13 PM (4 months, 10 days ago)

:nothingtoadd:


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Offlinemetalhead
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Re: cold sterilization [Re: dstark]
    #8692103 - 07/28/08 12:05 PM (4 months, 5 days ago)

Quote:

dstark said:
Because this question was covered many times,
Cold only slows growth.

If you have a amchine which does -274c` which is comjpletely freeze when the atoms stop moving then you might :wink:




no no i don't mean sterilize with cold i mean with a uv light cold meaning you don't have to boil it.


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OfflineBlackVenom
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Re: cold sterilization [Re: metalhead]
    #8692391 - 07/28/08 01:15 PM (4 months, 5 days ago)

I know that UV sterilizers kill bacteria, pathogens, and algae from my saltwater aquarium experience but im not sure if it will actually kill spores


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OfflineBlackVenom
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Re: cold sterilization [Re: BlackVenom]
    #8692406 - 07/28/08 01:21 PM (4 months, 5 days ago)

heres some info i found out...
UV works by using ultraviolet type C (germicidal) radiation to cause mutations in the organisms exposed to it. How well it works depend on a few conditions:
- length of exposure (longer is better)
- size of the organism (smaller, one-celled organisms affected most)
- clarity of your water (any small particles reduce effectiveness)
- ability of the organisms to get to the UV unit - and this is what a lot of people don't consider

Most UV units are outside the tank, as an in-line unit (tubing is connected to both sides and the water flows through either by being pumped or gravity fed). Since clarity is an issue, it should be passed through a filter before it goes through your UV.

As far as what you should expect from using one -

Algae - they will kill free-floating types (green water) and any reproductive spores, but if you have hair algae, diatoms, or cyanobacteria that are attached to your glass and substrate, the UV doesn't affect them - you have to scrape the glass or siphon these out yourself

Bacteria - will kill most (good or bad - UV doesn't discriminate!), although the bacteria needed for cycling your tank will be less affected since it attaches to surfaces.

Fungi - will kill free-floating forms and spores only

so theoretically, it might work


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Offlineagmotes165
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Re: cold sterilization [Re: BlackVenom]
    #8741048 - 08/07/08 09:43 PM (3 months, 26 days ago)

i think you would need to us a higher energy UV light, that produces significant amounts of UVC. I think they are sold as Germicidal UV lights and more info can be found on wikipedia under "Germicidal Lights" i think

I wanted to try this with higher energy sources, like xrays to sterilize bulk substrate...but everyone here thinks im crazy...:evil:


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OfflineMycoAu

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Re: cold sterilization [Re: agmotes165]
    #8743991 - 08/08/08 12:54 PM (3 months, 25 days ago)

If you want "cold sterilization" use syringe filters for liquid mediums and use gamma radiation or ethylene oxide for bulk materials.  Keep in mind, that gamma radiation is hazardous to your health (should be obvious), and ethylene oxide is poisonous (dangerous even at extremely low doses).  As a result, neither one is recommended outside of a commercial setting. 

Use a pressure cooker- safe, easy, cheap and readily available to pretty much everyone with access to this site.


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Offlineagmotes165
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Re: cold sterilization [Re: MycoAu]
    #8744109 - 08/08/08 01:15 PM (3 months, 25 days ago)

gamma is hard to come by, although xray tubes are expensive they can be bought and turned on and off, which eliminates alot of radioactive source isolation sheilding. But you still need shielding when ur using it  :notards:


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Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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