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 camplo
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 I inoculated agar, lc and rye all at the same time. (Hey nooby lookit here) 
#8668912 - 07/22/08 06:09 PM (3 years, 10 months ago) |
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I just wanted to encourage more noobs to go agar. Build a glovebox and use agar to start a spore syringe or print. Just do it. Now I'm not your average noob I did 2 months worth of digging on this site and reading some cultivation books before I even ordered some spores. I had no contams (on agar that is) and Ive done a handful of germinations and dozen plus transfers (practicing isolating a strain) so far. Some transfers were done very clumsy like too, but I never skimp on my room prep and the glovebox and loading prep. So with that experience under my belt my confidence has gone up and I'm starting my way up this ladder -> Spore syringe>agar>LC>Rye>Straw. I don't forsee contams being an issue anytime soon if ever. So this is me encouraging you (the noob more nooby than I am) to use agar.
Oh yeah I forgot to say. I inoculated (MSsyringe) agar, lc , and rye all in one sitting and only the agar germinated...
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Edited by camplo (07/22/08 06:10 PM)
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Damion5050
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Re: I inoculated agar, lc and rye all at the same time. (Hey nooby lookit here) [Re: camplo]
#8669068 - 07/22/08 06:45 PM (3 years, 10 months ago) |
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Good to hear you are having success I am a noob on my first grow.. I have 12 1/2pint brf cakes of golden teacher and 12 1 pint brf cakes of Koh samui... I ordered the syringes online then immediately made them into LC.. So I took 2 cc's of each syringe and turned it into roughly 8 oz or 236 cc's of liquid culture.. So now I have enough to make 20 syringes or each..
It's definantly a good way to start off, you should give it a shot.. You can pop it in the refrigerator for up to 6 months..
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camplo
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Re: I inoculated agar, lc and rye all at the same time. (Hey nooby lookit here) [Re: Damion5050]
#8720420 - 08/03/08 03:41 PM (3 years, 9 months ago) |
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I just wanted to say that the LC did eventually germinate like a week and a half later...
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BrandNewbie
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Re: I inoculated agar, lc and rye all at the same time. (Hey nooby lookit here) [Re: camplo]
#8720510 - 08/03/08 04:15 PM (3 years, 9 months ago) |
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Quote:
camplo said:
I don't forsee contams being an issue anytime soon if ever.
Careful! The shroom gods might be reading! You could get zapped for being so cocky.
Oh shroom gods, camplo regrets saying what he said. He knows that it is only because of your smiling down on him that he has thus far avoided any green meanies. He also wishes to stay in your good graces and avoid your wrath! 
(You're welcome bro...;))
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nugjug
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Re: I inoculated agar, lc and rye all at the same time. (Hey nooby lookit here) [Re: camplo]
#8720640 - 08/03/08 04:44 PM (3 years, 9 months ago) |
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I will second the move to agar. I avoided it for the longest time and tried to use liquid cultures to speed everything up but they were really hit or miss. It sucks when you wait forever for one to colonize only to find out it is contaminated. Moving to agar was the best decision I ever made. I only use a glovebox and and of the 60 plates I have poured so far only 2 have contaminated.
As far as the LC goes I'd recommend skipping it. Inoculate a few master jars with agar and use them for grain to grain transfers. Less chance of contamination, and it is easier to see if something is contaminated before you knock up a whole fleet of spawn with it.
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camplo
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Re: I inoculated agar, lc and rye all at the same time. (Hey nooby lookit here) [Re: nugjug]
#8744607 - 08/08/08 01:41 PM (3 years, 9 months ago) |
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Man.... I'm not perfect but I better good great results for the amount of discipline and attention to detail that I've put into this hobby. If it weren't for having the added task of sifting through misinformation/outdated preferred techniques versus what could be, etc etc etc I'd be on an even higher level of confidence.
I mean I really try not to miss anything.
I stay REAL liberal with the bleach spray. If I could I would put on a wet suit goggles air supply, inside of a complete solid surface room, Spray the room down with power sprayer feed with bleachwater, soak all unsterilized material with the same, sit in the room and read a laminated bleach soaked magazine for about 45minutes and then proceed with the inoculation.
Thats almost what I do but on a smaller scale but instead of spraying myself with BW I take a shower...
Sterilized tools get put into tinfoil purse sterilized.
All jars, tinfoil tool packs, insides of GB including glovebox bread bag arms! get saturated with BW and when GB is loaded its closed and I let it sit for a good 30 minutes.
For inoculations I just unscrew lid and inoculate(5 second rule wink wink RR ). I use babyfood jars for agar, lc , and plastic storage lids on quart canning jars for grain. Open tinfoil purse inside of glovebox when its time. Keep in mind that you never let anything that is considered sterile cant touch anything that is not sterile or you might was well consider it not sterile. And thank the (mushrooms gods as you like to call it) for rewarding those who work hard. Now even that doesn't mean that I cant get a contaminate. But given the work that I put into the craft I BETTER get good results or I shouldn't be doing the craft.
I think I'm going to do a tek for baby food jar lc's not that its revolutionary but it is really simple. No special lid needed one babyfood jar makes on 60 syringe of lc. I use homemade stirrer, chopped in half screws for bar. Because of strength of stirrer and shallowness of jar you can oxygenate that hell out of the liquid inside and and the spinning does not wet tyvek bandaid filter that I dampen with BW every time I stir or really every day or two just because. I do that to the filters of the agar and grain too. And I know thats overkill...or is it? Who cares....why not?
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Edited by camplo (08/08/08 01:51 PM)
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DJYoshaBYD
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Re: I inoculated agar, lc and rye all at the same time. (Hey nooby lookit here) [Re: camplo]
#8744637 - 08/08/08 01:46 PM (3 years, 9 months ago) |
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Agar was the best thing I ran across, because I could start isolating sub strains.. Before, I would have to rely on a mushy to clone from.. I only do agar culture about once per year, just to get a new strain..
the only bad thing is, you can isolate and isolate, then noc up some grain with that slant, then you bulk, then you fruit, and get a great flusher with no potency, or mad potency with no mushies.. lol.. basically, you isolate a few different sub strains, fruit them in separate trays, and see which ones perform better all together.. Then you should still have a master jar of the tray you fruited and liked.. Now you know that this grain jar has a great fruiting, very strong isolate.. its just like having a mama weed plant on hand all the time to take cuttings from..
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**Fuck cops: I dont cultivate anything**
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camplo
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Re: I inoculated agar, lc and rye all at the same time. (Hey nooby lookit here) [Re: DJYoshaBYD]
#8744680 - 08/08/08 02:01 PM (3 years, 9 months ago) |
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Right on, I think for my patience (low) and skill level that I would most benefit from cloning. I ALMOST isolated a strain one time and then started fucking up on my agar recipe and killed my transfers (not perfect am I). So now Im mad anxious to just reach fruiting.
I might start another thread on this but I was just wondering why people don't try and isolate out of clone tissue.
Also there has to be some old hand trade secretes on how to screen mycelium traits. For example some people will try to use their grain in their agar so that strains can go through the survival of the fittest from the get go.
I was thinking that using an over nutrient supplied agar recipe you would be better able to single out the fastest /hungriest mycelium. You might just get the same mycelial expression you get from using under feed agar recipes.
I also want to isolate rizos that I get on the glass of my agar jars and see what traits they might possess.
Comments
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Edited by camplo (08/08/08 02:03 PM)
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DJYoshaBYD
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Re: I inoculated agar, lc and rye all at the same time. (Hey nooby lookit here) [Re: camplo]
#8744697 - 08/08/08 02:09 PM (3 years, 9 months ago) |
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you want to start isolating from the actual spores themselves.. I just get a lil squirt on there, find the most rhizo part, transfer, find the most rhizo part, transfer.. I do this a few times till just super rhizo, then I transfer a slant to a quart of WBS, and let that grow.. then I spawn it to a bulk sub. In my case now, straight coir.. I usually have a few different isolated slants in different jars, and I will use a whole brick for 3-4 trays, and spawn with 1 quart of each different isolate..
then, by the time I finish fruiting, I have seen the traits, pro, and cons of all the experiments.. I document which one flushed the fullest, biggest, and the fastest.. then I have some friends try the fruits, and document what they felt... the one with the best average of size, flush, and potency will be the one that I grow for a year or so..
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**Fuck cops: I dont cultivate anything**
END MISINFORMATION!! READ AND LEARN BEFORE YOU SAY ANYTHING ABOUT ANYTHING!! IT HURTS OTHERS!!
"sucklesworth said: calling him the best at anything is just jeetarded"
"DJYoshaBYD said: Only a dumbass can overlay a substrate, cause its impossible."
DJYoshaBYD's Easy As Hell WBS/Coir Tek – Start to finish – Version 1.0
save the cheerleader.. save the world..
Im no troll.. I AM OGRE!!!
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camplo
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Re: I inoculated agar, lc and rye all at the same time. (Hey nooby lookit here) [Re: DJYoshaBYD]
#8744727 - 08/08/08 02:16 PM (3 years, 9 months ago) |
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I think I read an old hand quote stating something along the lines that strains that cause rhizo's to stick out of the casings tend to be good fruiter's? I read so damn much...
For the beginning of my venture I can settle for average potency? I'm sure its better than some shit I picked out the woods which tends to be hit and miss.
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DJYoshaBYD
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Re: I inoculated agar, lc and rye all at the same time. (Hey nooby lookit here) [Re: camplo]
#8744778 - 08/08/08 02:31 PM (3 years, 9 months ago) |
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yeah.. really, you will feel something.. they give different types of highs.. my B+ iso is a sonofabitch on your mind and body.. really mind boggling.. but the GT's before it were hella visual..
it will take a while to find out what "average potency is" cause it varies so much..
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**Fuck cops: I dont cultivate anything**
END MISINFORMATION!! READ AND LEARN BEFORE YOU SAY ANYTHING ABOUT ANYTHING!! IT HURTS OTHERS!!
"sucklesworth said: calling him the best at anything is just jeetarded"
"DJYoshaBYD said: Only a dumbass can overlay a substrate, cause its impossible."
DJYoshaBYD's Easy As Hell WBS/Coir Tek – Start to finish – Version 1.0
save the cheerleader.. save the world..
Im no troll.. I AM OGRE!!!
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camplo
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Re: I inoculated agar, lc and rye all at the same time. (Hey nooby lookit here) [Re: DJYoshaBYD]
#8744877 - 08/08/08 02:49 PM (3 years, 9 months ago) |
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Aw man don't talk like that... I just shook 10 jars of rye yesterday...I'm sooooo close...it hurts.
I think I don't have enough air exchange on my jars. First batch I used one 1/8th hole. Second batch I used 2 1/8th holes. But I cant make an accurate assumption of growth speed because I used 15cc vs 10cc so of course there's going to be faster growth with 15. I am scared of stalling the jars so yesterday I played some air exchange games. After doing some math and figuring out how much water each jar had lossed. My driest jar and least colonized jar is at 41% water content after 8 days. So it cool out yesterday, I jacked up temps in incubator to about 84f (it has no lid,
Check me out son
First batch I did 6 jars. 250grams including rh. Prepared that to 40%water content. Inoculated with 10ml of lc each.
I fucked that up by forgetting to shake syringe...duh duh duh. So I got one jar thats colonizing very well and one that is colonizing... well not very well.
Second batch... 4 jars... 280 grams of rye with water...42% water content. 15cc lc. I shook the syringe this time! Yea me! Shit looked 25% in 4 days. I probably could of gotten the jar to finish nearer to 6 days if I shook the jar better after inoculation. Lesson learned.
Thats the way to go.
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Edited by camplo (08/08/08 03:05 PM)
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DJYoshaBYD
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Re: I inoculated agar, lc and rye all at the same time. (Hey nooby lookit here) [Re: camplo]
#8744892 - 08/08/08 02:51 PM (3 years, 9 months ago) |
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shaking them constantly will fuck them.. you probably still good..
you dont want "air exchange" in jars.. you want "gas exchange".. a simple 1/4 inch hole in the lid with a filter of your choice, and that will suffice..
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**Fuck cops: I dont cultivate anything**
END MISINFORMATION!! READ AND LEARN BEFORE YOU SAY ANYTHING ABOUT ANYTHING!! IT HURTS OTHERS!!
"sucklesworth said: calling him the best at anything is just jeetarded"
"DJYoshaBYD said: Only a dumbass can overlay a substrate, cause its impossible."
DJYoshaBYD's Easy As Hell WBS/Coir Tek – Start to finish – Version 1.0
save the cheerleader.. save the world..
Im no troll.. I AM OGRE!!!
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willowmp
Medical Psychedelic Mushrooms
Registered: 07/25/08
Posts: 3,346
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Re: I inoculated agar, lc and rye all at the same time. (Hey nooby lookit here) [Re: DJYoshaBYD]
#8744906 - 08/08/08 02:54 PM (3 years, 9 months ago) |
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if i may ask how long do agar petri dishes last for. obviously if you leave them for a period of time there is a chance of contam but excluding the possibility of contam how long will they last. i know a few members have had fully colonized jars last months before being fruited, but jars do have a lot of nutrients compared to a slat of agar.
lets say how long do they last in a fridge and outside one.
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"Fuck it too much Im outta wittbhjkhbhkjsrglhkrgl g I DEE JEUD."
-Because it made sense on xanax and booze...
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DJYoshaBYD
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Re: I inoculated agar, lc and rye all at the same time. (Hey nooby lookit here) [Re: willowmp]
#8744930 - 08/08/08 02:58 PM (3 years, 9 months ago) |
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I just keep mine in a cupboard.. they last for months in there.. if you need to, you can put them in your fridge, but your fridge is actually very dirty... but I do this with fully colonized jars to store them if I know Im not using them..
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**Fuck cops: I dont cultivate anything**
END MISINFORMATION!! READ AND LEARN BEFORE YOU SAY ANYTHING ABOUT ANYTHING!! IT HURTS OTHERS!!
"sucklesworth said: calling him the best at anything is just jeetarded"
"DJYoshaBYD said: Only a dumbass can overlay a substrate, cause its impossible."
DJYoshaBYD's Easy As Hell WBS/Coir Tek – Start to finish – Version 1.0
save the cheerleader.. save the world..
Im no troll.. I AM OGRE!!!
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willowmp
Medical Psychedelic Mushrooms
Registered: 07/25/08
Posts: 3,346
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Re: I inoculated agar, lc and rye all at the same time. (Hey nooby lookit here) [Re: DJYoshaBYD]
#8744950 - 08/08/08 03:01 PM (3 years, 9 months ago) |
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o ok ty
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"Fuck it too much Im outta wittbhjkhbhkjsrglhkrgl g I DEE JEUD."
-Because it made sense on xanax and booze...
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DJYoshaBYD
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Re: I inoculated agar, lc and rye all at the same time. (Hey nooby lookit here) [Re: willowmp]
#8744980 - 08/08/08 03:06 PM (3 years, 9 months ago) |
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np
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**Fuck cops: I dont cultivate anything**
END MISINFORMATION!! READ AND LEARN BEFORE YOU SAY ANYTHING ABOUT ANYTHING!! IT HURTS OTHERS!!
"sucklesworth said: calling him the best at anything is just jeetarded"
"DJYoshaBYD said: Only a dumbass can overlay a substrate, cause its impossible."
DJYoshaBYD's Easy As Hell WBS/Coir Tek – Start to finish – Version 1.0
save the cheerleader.. save the world..
Im no troll.. I AM OGRE!!!
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camplo
Freedom!
Registered: 02/10/08
Posts: 488
Last seen: 1 month, 25 days
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Re: I inoculated agar, lc and rye all at the same time. (Hey nooby lookit here) [Re: DJYoshaBYD]
#8745013 - 08/08/08 03:14 PM (3 years, 9 months ago) |
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I had some jars in a gallon zip lock bags and then I came to the notion that they could suffocate and have since taken them out. Now maybe I'll have a chance to see what suffocated mycelium looks like. Or not. Who knows.
Fresh air. Gas. Very technical aren't we. Yeah, you're right.
Oh yeah um I had to convince myself to shake them. So scared to do anything wrong to them. I shook them once when they were first inoculated, with LC that is. Im pretty sure you're supposed to do that. And then you shake them once they get well enough colonized. And then they're usually colonized 3-4 days after first shake if you waited long enough to do so. Right!?
If so I'll have 4 jars 100% 8-9 days after inoculation and 6 other jars 100% after 12-13. Thats probably slow for the LC amounts I used. I Think its due to not shaking/ spreading lc around jar during/after the actual inoculation.
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Edited by camplo (08/08/08 03:21 PM)
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DJYoshaBYD
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Re: I inoculated agar, lc and rye all at the same time. (Hey nooby lookit here) [Re: camplo]
#8745021 - 08/08/08 03:17 PM (3 years, 9 months ago) |
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well, gas exchange and fresh air exchange are very different...
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**Fuck cops: I dont cultivate anything**
END MISINFORMATION!! READ AND LEARN BEFORE YOU SAY ANYTHING ABOUT ANYTHING!! IT HURTS OTHERS!!
"sucklesworth said: calling him the best at anything is just jeetarded"
"DJYoshaBYD said: Only a dumbass can overlay a substrate, cause its impossible."
DJYoshaBYD's Easy As Hell WBS/Coir Tek – Start to finish – Version 1.0
save the cheerleader.. save the world..
Im no troll.. I AM OGRE!!!
|
camplo
Freedom!
Registered: 02/10/08
Posts: 488
Last seen: 1 month, 25 days
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Re: I inoculated agar, lc and rye all at the same time. (Hey nooby lookit here) [Re: DJYoshaBYD]
#8745047 - 08/08/08 03:23 PM (3 years, 9 months ago) |
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Come on I said you were right.
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