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 baltazar
Quiet dreamer
Registered: 02/23/04
Posts: 671
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 Sunlight sterilization 
#8645742 - 07/17/08 03:50 AM (3 years, 10 months ago) |
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UV light with germicidal properties (called UVC), comes in 100-300 nm wavelenght range. UVC lamps have allready proven their effectiveness when it comes to sterilizing medical and other laboratory equipment. The Sun, ammong other wavelenghts, emits light in the UVC range. Although most of the UV light is absorbed by ozone in the upper layers of the atmosphere, a small ammount reaches Earth surface (the UVC wavelenght range is visible in the red frame in the picture).
I'm interested in using sunlight for spore syringe sterilization. A spore syringe would be made with no special sterile routines, and left on direct sunlight during a sunny day. What do you think, could it work? Or is the ammount of UVC in sunlight too low to have any use for sterilization...
Edited by baltazar (07/17/08 03:52 AM)
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 P.Menace
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 Re: Sunlight sterilization [Re: baltazar]
#8645822 - 07/17/08 05:06 AM (3 years, 10 months ago) |
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Are you trying to sterilize the spores too? Confusing the way you worded it...
Cause i think what your after here is sterilizing the water and then making a syringe... if your that hard up just use distilled water... its already sterile from the process of distillation...
Otherwise... no idea if it would work or not.
Id just microwave a half pint of water with the syringe inside(if *not* sterile) for like 3-5 minutes and let it cool down then make my syringes...
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http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace
roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.
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Pinback
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Re: Sunlight sterilization [Re: baltazar]
#8645837 - 07/17/08 05:17 AM (3 years, 10 months ago) |
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Considering the simple fact that most living things survive quite well in sunlight, this will not work.
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baltazar
Quiet dreamer
Registered: 02/23/04
Posts: 671
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Re: Sunlight sterilization [Re: P.Menace]
#8645866 - 07/17/08 05:39 AM (3 years, 10 months ago) |
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The sterility of my spore prints concern me, they are probably not 100% sterile. Having that in mind, your spore syringe making procedure could be perfectly done, but the spore water in thy syringe could still be contaminated because of the contaminated spore print. Exposing spore syringes to UVC light, after they have been done, could eliminate that problem...
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P.Menace
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Re: Sunlight sterilization [Re: baltazar]
#8645875 - 07/17/08 05:44 AM (3 years, 10 months ago) |
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I think if it could that "TEK" would be all over the place... however what you are talking about sounds like Reverse Ozmosis to me, and that really only kills bacteria to my knowledge
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http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace
roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.
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dstark
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Re: Sunlight sterilization [Re: P.Menace]
#8646025 - 07/17/08 07:02 AM (3 years, 10 months ago) |
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Nice idea 
Here in Israel some1 cooked eges in sunny day ^^
Well it might work here XD
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P.Menace
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Re: Sunlight sterilization [Re: dstark]
#8646030 - 07/17/08 07:04 AM (3 years, 10 months ago) |
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what are eges?
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http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace
roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.
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WeBeBad
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Re: Sunlight sterilization [Re: P.Menace]
#8646685 - 07/17/08 10:56 AM (3 years, 10 months ago) |
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I bet he meant eggs
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MYSTIQUE
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Re: Sunlight sterilization [Re: P.Menace]
#8646694 - 07/17/08 10:58 AM (3 years, 10 months ago) |
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OK this makes no sense to me.
Are the mushroom spores like super spores, stronger then any bacteria or other spores and endo spores?
Any sterilization you do to the spores will sterilize the spores in the process.
You clean the water before the spores go in it not after.
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Dont know what the fuck I just said? READ THIS
http://www.shroomery.org/5122/The-Shroomery-Mushroom-Glossary
I ain't a hippy but I'm covered in dirt
Sippin lots of mushroom tea in a tye-dye shirt
Chasin' the Grateful Dead, no shoes on my feet
Beggin' in the parking lot for something to eat,
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robanero
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Re: Sunlight sterilization [Re: MYSTIQUE]
#8646853 - 07/17/08 11:45 AM (3 years, 10 months ago) |
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Did you make the prints yourself?
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310 open cap Hawaiians grown a 1 pint WBSF cake.
SPAWN RATIO CALCULATOR
My Little Hawaiians
Spawn Bag Tek
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P.Menace
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Re: Sunlight sterilization [Re: robanero]
#8646887 - 07/17/08 11:54 AM (3 years, 10 months ago) |
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If the prints are suspected to be contaminated then the only way to deal with it is agar, germinate out your spores and isolate away from contaminations...
The Only Way...
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http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace
roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.
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ch4v4
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Re: Sunlight sterilization [Re: P.Menace]
#8646979 - 07/17/08 12:16 PM (3 years, 10 months ago) |
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Interesting, this could be used in the fruiting chamber. useful for sterilizing the spores? you better read this:
"In the reduced-pressure (<10 torr) afterglow stemming from discharges in O2-containing mixtures such as N2-O2, the test-reference spores are ultimately inactivated by UV photons through destruction of their genetic material (DNA). To show this, we assume the inactivation to result from a sufficiently large number of successful hits of the DNA strands by UV photons. This implies that the higher the UV intensity, the shorter the time required to reach the lethal dose. Simultaneously, the increased erosion of the spores by the oxygen atoms as time elapses reduces the incident number of photons required to meet the lethal dose. Erosion, as observed by scanning electron microscopy, also increases with the O2 percentage in the mixture. Actually, sterilization time is found to be the shortest when the O2 percentage in the mixture is set to maximize the UV emission intensity, which occurs at O2 percentages typically below 2%, where erosion is low. This proves the predominant role of UV radiation over erosion as far as spore inactivation is concerned. In any case, plasma sterilization always implies some erosion of the test spores, in contrast to what happens with conventional sterilization methods."
http://cat.inist.fr/?aModele=afficheN&cpsidt=14487633
AND
Moreover, inactivation is quite fast: 106 B. atrophaeus spores deposited on a Petri dish are inactivated in less than 1 min.
http://www.iop.org/EJ/abstract/0022-3727/41/13/135212
EDIT: URL's
Edited by ch4v4 (07/17/08 12:18 PM)
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RogerRabbit
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Re: Sunlight sterilization (moved)
#8647587 - 07/17/08 02:33 PM (3 years, 10 months ago) |
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 This thread was moved from Advanced Mycology.
Reason:
Off topic in advanced mycology. Moved to cultivation
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roquet
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Re: Sunlight sterilization (moved) [Re: RogerRabbit]
#8647713 - 07/17/08 02:56 PM (3 years, 10 months ago) |
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waixingren did an experiment pasteurising poo in sunlight. Not sure how it turned out. I'd love to be able to sterilise my grain without using the stove.
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ray40cal
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Re: Sunlight sterilization [Re: baltazar]
#8647814 - 07/17/08 03:14 PM (3 years, 10 months ago) |
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Quote:
baltazar said:
I'm interested in using sunlight for spore syringe sterilization. A spore syringe would be made with no special sterile routines, and left on direct sunlight during a sunny day. What do you think, could it work? Or is the ammount of UVC in sunlight too low to have any use for sterilization...
Not 100% sure what you mean by this. Let's assume that leaving things under direct sunlight does make them sterile. Even if you did sit a syringe under direct sunlight, the only thing about it that would become sterile is the needle. Nothing else on the syringe touches your substrate but the needle. You can easily sterilize a needle with a flame or alcohal. Now if you're talking about sterilizing the water that's in the syringe, then that probably wouldn't work. Water left under the sun doesn't become sterile. Still assuming the sun would sterilize the water, it would be quicker to just boil it anyways. You want to sterilize the inside of the syringe too so boiling water is probably a better choice.
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