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 SuchSmartMonkeys
mycologicallydrivenindividual
Registered: 10/26/05
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 oysters stalled, then starting to get fluffy?
#8589203 - 07/02/08 08:52 PM (1 month, 26 days ago) |
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okay, so these oyster blocks have been bugging me ever since i started them... some of them started fruiting straight through the filter patch before fully colonizing the bottoms, the ones that didn't i stuck in my FC.
The problem: Basically started out by just cutting a few holes in each bag, hoping they would just fruit out of the holes. of course, they started spitting out mushies in different spots, so i cut the whole bags off and placed the blocks in my FC (i think they dried out a bit before they made it to the FC). After a few days of being in the FC, they started spitting out tons more pins. Then the whole thing just stopped. it's been stalled for about a week, still been getting a lot of AE until yesterday, it got absolutely none all day... (i'm almost positive the problem is the heat, but my digi therm/hygrometer broke, so i can't quite tell)
i just opened it up after not looking at it for about 36 hours, and the mushies have begun growing again (long in the stem, no AE...) but there is also some odd puff/fuzziness showing up on the blocks. Some of it it spreading across the perlite on the bottom of my FC. Is this just cobweb, a little h202 spritz will fix? or possibly mycelial growth? any other ideas?
pics:
I just thought these last were cool pics, you can see some of the puffiness in the bottom pic...
and on a completely unrelated note, here is a pic of my new glovebox in the making! YAY!
i accidentally broke the piece of glass i was going to put into that hole in the top, but i've got another, i just need to cut it... then i will be doing agar work in the home lab starting tomorrow!!
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Mycelio
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Re: oysters stalled, then starting to get fluffy? [Re: SuchSmartMonkeys]
#8590330 - 07/03/08 02:57 AM (1 month, 25 days ago) |
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That is no heat problem.
Your blocks are heavily contaminated plus they got too little fresh air, not only in the past 36hrs. I'd only put two of those blocks in that FC.
As you suggest, the main contam seems to be cobweb, but there is probably more growing on your oysters.
Do you have a garden? You may have a chance if you dig a hole in a shady spot, put the blocks in and cover them with an inch of soil. Covering with an additional inch of hay or straw will make it easier to keep it moist.
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waixingren
I am the FrizzleFry
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Re: oysters stalled, then starting to get fluffy? [Re: Mycelio]
#8590679 - 07/03/08 06:55 AM (1 month, 25 days ago) |
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they dont look contaminated to me. you need to open up your fruiting chamber and let the air flow more freely. oysters need lots and lots of fresh air and light. give them some flow and the fruits will begin to form normally.
--------------------
"The real secret of magic is that the world is made of words. And that if you know the words that the world is made of, you can make of it whatever you wish. "
-T.M.
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Mycelio
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Re: oysters stalled, then starting to get fluffy? [Re: waixingren]
#8592607 - 07/03/08 06:18 PM (1 month, 25 days ago) |
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To me the fluffy parts do not look like oyster-mycelium, even if it would be possible to grow into the air, when humidity and CO2 are high enough. Obviously, the mycelium is too wet and unable to form caps, due to a CO2 overdose.
Well, I hope I am wrong with the cobweb, but then there are also these brown and gray areas, especially on the block at the top right. Healthy mycelium should be white. Orange drops appear sometimes without a visible contamination. I always take them as a hint for bacteria.
Anyway, if the blocks shall stay inside, I also recommend fresh air and less humidity. Water on the blocks is an invitation for contams.
Edited by Mycelio (07/03/08 06:20 PM)
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 lipa
Registered: 07/24/07
Posts: 402
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Re: oysters stalled, then starting to get fluffy? [Re: SuchSmartMonkeys]
#8592886 - 07/03/08 07:52 PM (1 month, 25 days ago) |
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Trash them there gone. Start over.
Next time initiate earlier. When the mycelium "almost" completely looks colonized stick them outside in a moist shady area. I agree with mycelio thats way too much mycelium for that fruiting container. Oysters generate "lots of Co2". If you want to use those tubs, only cover them half way, drill lots of holes under the perlite to allow air to follow through, and don't mist too much. Those things are drenched!
Resist pealing the plastic off. If you want a cluster to break through just carefully slit in front of it like +. Taking the plastic off is just going to help dry the colony out. You can open the top and encourage most of your flush to occur on top too.
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SuchSmartMonkeys
mycologicallydrivenindividual
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Re: oysters stalled, then starting to get fluffy? [Re: lipa]
#8592945 - 07/03/08 08:20 PM (1 month, 25 days ago) |
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nope, they aren't trash, the main constituent of my substrate is coffee, which fully explains the brown spots... The blocks are for sure fully colonized, and the only contam i'm worried about is that fluffiness... which sounds like the general consensus is cobweb? i'll spray it with a little h202? i haven't been misting at all, the largest problems seem to be too much moisture and not enough air exchange... i'll prop the lid open and see how it does for a few days.
edit: the one all the way up in the upper left is on a different substrate. Me and my girlfriend wanted to do a test to see if scotchbroom was a suitable substrate. first flush was fucking amazing, but after dunking, it seemed like the mycelium receded a bit on the upper parts of the log. it continued to produce pins in this area though, lots of them, but did the same thing as the others and stalled... i've got the top of my tub propped open now though
Edited by SuchSmartMonkeys (07/03/08 08:24 PM)
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FreeSporePrints
stronzo chilegge
Registered: 03/06/05
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Re: oysters stalled, then starting to get fluffy? [Re: SuchSmartMonkeys]
#8594028 - 07/04/08 04:20 AM (1 month, 24 days ago) |
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Consider this flush gone. Re-soak the blocks in water and then put them into your fruity chamber.
For what i see your blocks needs:
1) light
2) air
Let us know,
Fabio
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Mycelio
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Re: oysters stalled, then starting to get fluffy? [Re: FreeSporePrints]
#8594245 - 07/04/08 08:04 AM (1 month, 24 days ago) |
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Soaking may not be necessary. These blocks are dripping wet already, trying to sweat out as much water as they can.
My straw, wood and coffee blocks never needed any watering. By digesting straw, wood and coffee, a lot of water is produced, as well as CO2.
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lipa
Registered: 07/24/07
Posts: 402
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Re: oysters stalled, then starting to get fluffy? [Re: SuchSmartMonkeys]
#8594430 - 07/04/08 10:16 AM (1 month, 24 days ago) |
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Well either way I wish you the best! Good Luck.
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FreeSporePrints
stronzo chilegge
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Re: oysters stalled, then starting to get fluffy? [Re: Mycelio]
#8594545 - 07/04/08 11:15 AM (1 month, 24 days ago) |
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Quote:
Mycelio said:
Soaking may not be necessary. These blocks are dripping wet already, trying to sweat out as much water as they can.
My straw, wood and coffee blocks never needed any watering. By digesting straw, wood and coffee, a lot of water is produced, as well as CO2.
Is not necessary but helps the block to fruit powerfully the next flushes. Those primordia are made of water and they're a loss of water for the block.
If your blocks produce water i believe you spray them too much and the environment in the fruity chamber is too much humid.
The goal is to create humidity, but not to permit the little micro-drops of humidity to join each others and create drops of water. Is quite difficult but it's the rule!
Fabio
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Mycelio
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Re: oysters stalled, then starting to get fluffy? [Re: FreeSporePrints]
#8595252 - 07/04/08 03:13 PM (1 month, 24 days ago) |
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Fabio, I agree to watering if the mycelium dries out, but I never observed it, though besides never dunking I also never sprayed my blocks directly. It seems I was lucky and my humidity was just right to balance produced water and evaporation. As I was using flower pots instead of plastic bags, evaporation must have been reduced in my case.
Regarding biological efficiency, oysters have a maximum of ca. 200%, which means up to 20% of the dry substrate mass can be turned into fruitbodies. From the remaining 80% most of the carbohydrates are converted into water and CO2. So even if the mushrooms contain 90% water, while the substrate had ca. 75% initially, we theoretically have more than enough water. It just depends on how much is lost by evaporation.
Carsten
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SuchSmartMonkeys
mycologicallydrivenindividual
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Re: oysters stalled, then starting to get fluffy? [Re: Mycelio]
#8595440 - 07/04/08 04:12 PM (1 month, 24 days ago) |
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consider this flush lost.... okay, so should i pick off the pins, or just leave them, it'll be tough to remove all of them, there are tons of tiny little buggers all over the fucking place... and it sounds like general consensus is that they probably don't need a dunk? should i just pluck off what there is and leave them in the FC?
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Mycelio
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Re: oysters stalled, then starting to get fluffy? [Re: SuchSmartMonkeys]
#8595556 - 07/04/08 05:01 PM (1 month, 24 days ago) |
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I would remove all the stalled pins and leave the blocks in there. You can dunk them after the next flush if they dry out.
Did the fluffy stuff stop growing?
Carsten
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SuchSmartMonkeys
mycologicallydrivenindividual
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Re: oysters stalled, then starting to get fluffy? [Re: SuchSmartMonkeys]
#8596383 - 07/05/08 12:38 AM (1 month, 23 days ago) |
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the fluffy stuff hasn't really grown any more, but it hasn't yet gone away; i'll probably give a little spritz of some H2O2 tomorrow... i was thinking tomorrow i'd probably snip off the pins that looked like aborts and leave all the ones that look like they are still growing on there for now...
edit: broke my other piece of glass while i was trying to cut it earlier also, haha. oops, i've no luck with that shite. I'm going to go to a place where they sell salvaged/used items, see if i can't find myself a nice piece of plexiglass or something, figure that'll be quite a bit easier to work with.
Edited by SuchSmartMonkeys (07/05/08 12:42 AM)
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Mycelio
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Re: oysters stalled, then starting to get fluffy? [Re: SuchSmartMonkeys]
#8597138 - 07/05/08 11:42 AM (1 month, 23 days ago) |
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Perhaps we can try to find out if the fluffy bits are cobweb or oyster mycelium, before you pull out the H2O2.
So, does it look like the fluffy stuff is digesting the pins?
Carsten
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SuchSmartMonkeys
mycologicallydrivenindividual
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Re: oysters stalled, then starting to get fluffy? [Re: Mycelio]
#8599354 - 07/06/08 12:44 AM (1 month, 22 days ago) |
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hard to say, the thing that leads me most to believe it isn't oyster mycelium is that there is a remote colony separate from any o the blocks growing on the perlite. this seems a little suspect.
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Mycelio
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Re: oysters stalled, then starting to get fluffy? [Re: SuchSmartMonkeys]
#8599526 - 07/06/08 01:40 AM (1 month, 22 days ago) |
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Yes, that's odd, but it could be connected to a block under the surface. When I had blocks on perlite, the mycelium was sometimes growing into it.
I have never seen cobweb on oyster blocks, but I would expect it to be more aggressive. By now it should be everywhere, visibly digesting your blocks.
Does anybody have more experience with cobweb and can give us a hint?
Carsten
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SuchSmartMonkeys
mycologicallydrivenindividual
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Re: oysters stalled, then starting to get fluffy? [Re: SuchSmartMonkeys]
#8599565 - 07/06/08 02:00 AM (1 month, 22 days ago) |
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yeah, i haven't sprayed with any h2o2 yet, and it hasn't spread any more...
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Mycelio
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Re: oysters stalled, then starting to get fluffy? [Re: SuchSmartMonkeys]
#8729320 - 08/05/08 02:21 PM (23 days, 9 hours ago) |
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So what happened to the fluffy oysters?
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