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 wisp
Registered: 04/13/08
Posts: 5,304
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 . *DELETED* *DELETED* 
#8552417 - 06/22/08 09:34 PM (3 years, 10 months ago) |
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Post deleted by wispReason for deletion: .
Edited by wisp (09/08/11 07:26 PM)
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 dstark
Yes you can but you may Not!
 
Registered: 02/27/08
Posts: 3,075
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Re: Failed cloning from button mushroom [Re: wisp]
#8553078 - 06/23/08 02:47 AM (3 years, 10 months ago) |
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Same here tripsis ;(
I made about 5 clones, non of them survived - same story as yours!
Then i made spore print from shop button and as you know sterility in shops is 0% so around most spores became contam!
Other spores started germinating only after 3 week :O :S
Well maybe that the secret of comerical shops for shrooms xD
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wisp
Registered: 04/13/08
Posts: 5,304
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Re: Failed cloning from button mushroom [Re: dstark]
#8553081 - 06/23/08 02:49 AM (3 years, 10 months ago) |
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You actually got button mushrooms spores to germinate? Wow, that's supposed to be really difficult. I took some fresh prints so I'm going to put some spores on agar this week. Hopefully I'll be able to get them to germinate too.:)
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dstark
Yes you can but you may Not!
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Re: Failed cloning from button mushroom [Re: wisp]
#8553121 - 06/23/08 03:23 AM (3 years, 10 months ago) |
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GL but be ready to wait 
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primordius77
The BEEKEEPER
Registered: 05/30/08
Posts: 20
Loc: South Africa
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Re: Failed cloning from button mushroom [Re: dstark]
#8554266 - 06/23/08 11:37 AM (3 years, 10 months ago) |
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tripsis,
Maybe you should try a different agar medium - Say if you used PDA then use some MA or MYA.
I once got some button mushroom spawn (a few grains) and tried it on PDA - Very very very slow growth....
The agar plates dried out before the culture could get going properly.
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flavoraid
now with twicethe ketamine andopiates!
Registered: 12/05/07
Posts: 1,678
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Re: Failed cloning from button mushroom [Re: primordius77]
#8653741 - 07/19/08 12:03 AM (3 years, 10 months ago) |
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what was the source of your starting material? (ex. spores from xxx, culture from xxx, ect.)
theres probably not a whole lot of agaricus cultivators on this forum I remember reading posts of RR saying he couldn't get his to fruit when he needed them for a video.. that made me not want to try growing agaricus mushrooms.
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coda said:
imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.
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dstark
Yes you can but you may Not!
Registered: 02/27/08
Posts: 3,075
Loc:
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Re: Failed cloning from button mushroom [Re: primordius77]
#8654109 - 07/19/08 04:04 AM (3 years, 10 months ago) |
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Quote:
primordius77 said:
tripsis,
Maybe you should try a different agar medium - Say if you used PDA then use some MA or MYA.
I once got some button mushroom spawn (a few grains) and tried it on PDA - Very very very slow growth....
The agar plates dried out before the culture could get going properly.
Dried :/ well if you put parafilm they wont
Well i used also PDA and was very very slow! RR is cloning on agar with malt extract, i supose its batter ^^
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falcon
In the green
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Re: Failed cloning from button mushroom [Re: wisp]
#8656543 - 07/19/08 07:24 PM (3 years, 10 months ago) |
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How long have the chunks of mushroom been on agar?, sometimes bisporus takes 10 to 14 days before you see growth.
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dstark
Yes you can but you may Not!
Registered: 02/27/08
Posts: 3,075
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Re: Failed cloning from button mushroom [Re: falcon]
#8657128 - 07/19/08 10:42 PM (3 years, 10 months ago) |
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huh, Clones was failed, germination was from spores xD
took almost 1 month to germinate hahah xD lol!
then the agar was dry 
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wisp
Registered: 04/13/08
Posts: 5,304
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Re: Failed cloning from button mushroom [Re: dstark]
#8657342 - 07/20/08 12:12 AM (3 years, 10 months ago) |
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Yeah, I posted a bit prematurely. It worked in the end, not all the pieces of tissue, but several. Still have it all on plates though,growth is so slow. I'll start some jars of spawn tomorrow though.
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C. duckie
Stranger
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Re: Failed cloning from button mushroom [Re: wisp]
#8667582 - 07/22/08 11:34 AM (3 years, 10 months ago) |
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maybe if you did the stab the stem tek and made a lc clone it work better
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might show tits for prints ;o)
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wisp
Registered: 04/13/08
Posts: 5,304
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Re: Failed cloning from button mushroom [Re: C. duckie]
#8670703 - 07/23/08 05:29 AM (3 years, 9 months ago) |
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I don't see how it would make a difference how the tissue was taken from the mushroom. As I said, it did work out, it's just Agaricus mycelium is very slow.
I'm not keen on the idea on cloning straight to liquid culture. I prefer cloning to agar to ensure the culture is clean, then proceeding to LC later.
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Hotnuts
old hand
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Re: Failed cloning from button mushroom [Re: wisp]
#8670828 - 07/23/08 07:20 AM (3 years, 9 months ago) |
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"I'm not keen on the idea on cloning straight to liquid culture. I prefer cloning to agar to ensure the culture is clean, then proceeding to LC later. "
Good thinking.
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cutemushie
Mushrooms are fascinating.
Registered: 05/31/08
Posts: 130
Loc: Aus
Last seen: 10 months, 7 days
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Re: Failed cloning from button mushroom [Re: wisp]
#8706433 - 07/31/08 07:39 AM (3 years, 9 months ago) |
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Quote:
tripsis said:
Hi, I have been growing some button mushrooms. As I want to continue this I decided to clone off one. I did so yesterday and placed a small piece of tissue of several agar plates. Unexpectedly though, the tissue has turned brown and appears to have died.
My question is, is this somehow due to senescence of the strain because it is commercially grown? I tried cloning off shop bought button mushrooms with the same results, but I attributed the failure to the fact the mushrooms were days old. This one was most definitely alive when I cloned it.
Is it normal for it to go brown like that and can I still expect to see growth at some point?
Thanks for any feedback.
I have tried cloning some other mushrooms on agar before and quite often, the tissue would turn brown and soggy and look dead but after a while, mycelium will grow from it. Sometimes however, it will not grow mycelium. Just my personal experience.
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HAVE: Paneolus cyanescence, Paneolus cambodgiensis, Psilocybe cubensis, Flammulina velutipe (white, and brown Korean strain), Hericium erinaceous, Coprinus atramentarius, Coprinus comatus, Agrocybe aegerita, Pleurotus eryngii, Psilocybe subaeruginosa, Ganoderma lucidum, Pleurotus nebrodensis, Lepista nuda, Agaricus augustus, Pleurotus cystidiosus, Volvariella speciosa.
LOOKING FOR: Those that I don't have.
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dstark
Yes you can but you may Not!
Registered: 02/27/08
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Re: Failed cloning from button mushroom [Re: cutemushie]
#8711477 - 08/01/08 09:41 AM (3 years, 9 months ago) |
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Same here
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wisp
Registered: 04/13/08
Posts: 5,304
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Re: Failed cloning from button mushroom [Re: dstark]
#8713695 - 08/01/08 06:29 PM (3 years, 9 months ago) |
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Yeah, it was quite strange actually. Many of the pieces of tissue that went brown, ended up showing growth, while the pieces that stayed firm and white did not.
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lorbitherize
Stranger
Registered: 11/24/05
Posts: 328
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Re: Failed cloning from button mushroom *DELETED* [Re: wisp]
#8721898 - 08/03/08 09:41 PM (3 years, 9 months ago) |
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Post deleted by lorbitherizeReason for deletion: ...
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dstark
Yes you can but you may Not!
Registered: 02/27/08
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Re: Failed cloning from button mushroom [Re: lorbitherize]
#8721956 - 08/03/08 10:00 PM (3 years, 9 months ago) |
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I thought by myself the PDA arnt the best option for edibles, well i saw RR recomend malt extract agar.
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Peterthinks
(Caulking) gun for hire
Registered: 11/10/04
Posts: 2,377
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Re: Failed cloning from button mushroom [Re: dstark]
#8748138 - 08/09/08 09:02 AM (3 years, 9 months ago) |
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My button mushroom clone didn't work either (on PDA), since it is a dung lover I'd suggest sterilizing some horse manure in a jar and throwing your tissue clone in there.Experiment with water content for best results.
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Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.
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Hotnuts
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Re: Failed cloning from button mushroom [Re: Peterthinks]
#8748318 - 08/09/08 09:57 AM (3 years, 9 months ago) |
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PDA works fine for me with every mushroom i've cultured, Agaricus' included.
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