|
 the_0wl
The Space Bird
Registered: 04/15/08
Posts: 442
Last seen: 2 years, 9 months
|
 Anything look wrong with these rye jars? (pics) Not growing. . 
#8467819 - 05/31/08 02:51 PM (3 years, 11 months ago) |
|
|
So I soaked the rye for 24 hours, strained them and washed it. Then simmered/boiled it all in a large pot for about 45 min then strained and rinsed it again. Then put them into the jars and pressure cooked for 1 hour at 15 psi.
I inoculated them with a spore syringe from T.H.E. that I had refrigerated for a week or so..I let it warm up to room temp before injecting though. I also let the PC and jars cool over night.
I'm not using Tyvek filters on the lids though. I'm Just using the canning jar lids with tin foil over them. There are 2-4 holes in all the jar lids from where I inoculated. Theres no signs of contam at all in any of them..but no sign of colonization either. Its nearly been 2 weeks now. Anyone ever have colonization this slow or should I just try again?
I'm not sure what could be wrong really. Other then perhaps being really dry..There seems to be no moisture in the jars at all really..or is that how its supposed to be? Other then the water the rye seeds absorbed I guess.
--------------------
Everything I say on this website and forum is completely false and fantasy role play. In reality I do not engage in any illegal activities regardless of what my posts say.
|
the_0wl
The Space Bird
Registered: 04/15/08
Posts: 442
Last seen: 2 years, 9 months
|
Re: Anything look wrong with these rye jars? (pics) Not growing. . [Re: the_0wl]
#8472311 - 06/01/08 07:12 PM (3 years, 11 months ago) |
|
|
Still nothing. . .
--------------------
Everything I say on this website and forum is completely false and fantasy role play. In reality I do not engage in any illegal activities regardless of what my posts say.
|
 dumbfounded1600
Stranger
Registered: 07/29/07
Posts: 2,624
|
Re: Anything look wrong with these rye jars? (pics) Not growing. . [Re: the_0wl]
#8472329 - 06/01/08 07:17 PM (3 years, 11 months ago) |
|
|
No, they look fine.
What temp are you incubating at?
Your procedure seems ok I guess...I would change it by adding gypsum but that's just me.
They're supposed to show no visible moisture on the kernals themselves but inside is where it's at..honestly they look good.
Where did you buy your spore syringe at?
|
Neobean
Adept Mycologist
Registered: 10/07/01
Posts: 975
Loc: Canada
Last seen: 1 year, 4 months
|
Re: Anything look wrong with these rye jars? (pics) Not growing. . [Re: the_0wl]
#8472383 - 06/01/08 07:36 PM (3 years, 11 months ago) |
|
|
If you simmered for 45 minutes, there is no chance in hell that your rye berries would be too dry. If the tin foil is too tightly wrapped around your jar, you might be restricting gas exchange...other than this, you might be looking at a bad syringe? Time will tell.
--------------------
If y0u want s0meting gr0wn right, y0u g0tta gr0w it y0urself!!!
|
The shroomy 1
Luminous beings surround me
Registered: 03/27/07
Posts: 5,054
Loc: Texas
|
Re: Anything look wrong with these rye jars? (pics) Not growing. . [Re: Neobean]
#8472431 - 06/01/08 07:49 PM (3 years, 11 months ago) |
|
|
Two weeks seems to be a bit much. The top part of the jars seem to be drying out already. You should have seen something already. The only thing that I can offer is that putting your syringes in the fridge is going to put your spores in a state of dormancy. They may take some time to recover from that state to a viable state where they can germinate. I would have pulled out the syringes from the fridge about a week or before the preparation of the jars. There is no visible sign of contamination, so you still have a shot at this. Hang in there.
The Shroomy 1
--------------------
|
the_0wl
The Space Bird
Registered: 04/15/08
Posts: 442
Last seen: 2 years, 9 months
|
Re: Anything look wrong with these rye jars? (pics) Not growing. . [Re: The shroomy 1]
#8472502 - 06/01/08 08:07 PM (3 years, 11 months ago) |
|
|
The syringe came from The Hawks Eye. The first 2 I got from him were good. I have no reason to think the syringe was bad. .unless refrigerating them prolonged things alot. I'm removing the tinfoil and loosening the lids a bit for air exchange..perhaps that was the deal..the foil was on tight and the lids were kinda tight too. Much longer with nothing and I dont think I can come to any other conclusion then a bad syringe though...
--------------------
Everything I say on this website and forum is completely false and fantasy role play. In reality I do not engage in any illegal activities regardless of what my posts say.
|
dumbfounded1600
Stranger
Registered: 07/29/07
Posts: 2,624
|
Re: Anything look wrong with these rye jars? (pics) Not growing. . [Re: the_0wl]
#8472769 - 06/01/08 09:08 PM (3 years, 11 months ago) |
|
|
I would blame it on Aluminum Foil...and not having a proper filter.
|
the_0wl
The Space Bird
Registered: 04/15/08
Posts: 442
Last seen: 2 years, 9 months
|
Re: Anything look wrong with these rye jars? (pics) Not growing. . [Re: dumbfounded1600]
#8472933 - 06/01/08 09:40 PM (3 years, 11 months ago) |
|
|
So the tyvek filter is terribly necessary then?
--------------------
Everything I say on this website and forum is completely false and fantasy role play. In reality I do not engage in any illegal activities regardless of what my posts say.
|
dumbfounded1600
Stranger
Registered: 07/29/07
Posts: 2,624
|
Re: Anything look wrong with these rye jars? (pics) Not growing. . [Re: the_0wl]
#8472958 - 06/01/08 09:45 PM (3 years, 11 months ago) |
|
|
Honestly, I can't vouch for tyvek. I only use Synthetic Filter Disks for it is far more superior and an actual micron filter. But what I do know though is if you use Tyvek...cover it up 90% of the surface are with duct/masking tape so it doesn't dry out your grain jars because of it's so thin. It'll still stop contaminants without it but it'll likely dry out your spawning material before full colonization.
|
itsallnines
mush-head
Registered: 04/17/07
Posts: 385
Last seen: 3 months, 3 days
|
Re: Anything look wrong with these rye jars? (pics) Not growing. . [Re: dumbfounded1600]
#8473211 - 06/01/08 11:08 PM (3 years, 11 months ago) |
|
|
you don't see any moisture in the jars at all? that is odd being that you simmered for 45 minutes. i always see some condensation. not much, but some.
inoculating with spores, in my experience, is a little tricky, in that they sometimes take a LONG time to show any kind of growth.
you may want to try making a liquid culture. there are several teks on this, but if you want to know how i do it and/or want a picture of my liquid culture that i started from spores, let me know.
--------------------
by their fruits ye shall know them.
|
Prisoner#1
Even Dumber ThanAdvertized!
Registered: 01/22/03
Posts: 152,616
Loc: Pvt. Pubfag NutSuck 
|
Re: Anything look wrong with these rye jars? (pics) Not growing. . [Re: dumbfounded1600]
#8474079 - 06/02/08 06:54 AM (3 years, 11 months ago) |
|
|
Quote:
dumbfounded1600 said:
I would blame it on Aluminum Foil...and not having a proper filter.
I wouldnt, it needs to start colonizing before gas exchange
would be a problem, since it's not colonizing I dont believe
foil would be an issue nor would the filter
Quote:
dumbfounded1600 said:
Honestly, I can't vouch for tyvek. I only use Synthetic Filter Disks for it is far more superior and an actual micron filter.
I've used both, had more contams using the filter disks than the
tyvek, but as you mentioned, tyvek can allow a little too much
moisture out, just as the filter disks can, the synthetic filter
disks can also allow contams to be wicked in if they get
wet/damp/moist, thickness of the disk has no effect on it's abilities
--------------------
|
dstark
Yes you can but you may Not!
Registered: 02/27/08
Posts: 3,075
Loc: 
Last seen: 1 month, 6 days
|
Re: Anything look wrong with these rye jars? (pics) Not growing. . [Re: Prisoner#1]
#8474118 - 06/02/08 07:24 AM (3 years, 11 months ago) |
|
|
Maybe you should reliese the lid! maybe its to tigh!
|
Psilocelium
Size of @n Ant
Registered: 01/19/08
Posts: 963
Loc: ----_---_--_
Last seen: 3 days, 2 hours
|
Re: Anything look wrong with these rye jars? (pics) Not growing. . [Re: dstark]
#8474330 - 06/02/08 09:15 AM (3 years, 11 months ago) |
|
|
I found that Stametts recipe worked perfectly for me the first time, and has not worked since. Rye grain 100% clonization in 5 days.
I'm back with WBS for now.
Fungi Perfecti has a good collection of cultivation books with sound procedures, I just don't have an entire room solely dedicated to sterility and cultivation.
--------------------
THIS IS MY HOME-
--------------------Give Thanks To Your Creator-----------------------
All Posts Are Fictional and for Entertainment Purposes
|
dumbfounded1600
Stranger
Registered: 07/29/07
Posts: 2,624
|
Re: Anything look wrong with these rye jars? (pics) Not growing. . [Re: Psilocelium]
#8474485 - 06/02/08 10:15 AM (3 years, 11 months ago) |
|
|
Quote:
I wouldnt, it needs to start colonizing before gas exchange
would be a problem, since it's not colonizing I dont believe
foil would be an issue nor would the filter
The foil would restrict Gas Exchange depriving the myc to grow/branch out.
How big are your filters 'the_oWl'?
Quote:
I've used both, had more contams using the filter disks than the
tyvek, but as you mentioned, tyvek can allow a little too much
moisture out, just as the filter disks can, the synthetic filter
disks can also allow contams to be wicked in if they get
wet/damp/moist, thickness of the disk has no effect on it's abilities
How is that possible? What size were your lid holes?
|
shroober
Myco Junkie
Registered: 01/02/08
Posts: 879
|
Re: Anything look wrong with these rye jars? (pics) Not growing. . [Re: dumbfounded1600]
#8474804 - 06/02/08 11:39 AM (3 years, 11 months ago) |
|
|
dumb, lack of gas exchange may stall or slow a colonizing jar but it will not prevent germination. there is sufficient oxygen for germination in the jar even with a closed lid. obviously it's not ideal to have a entirely closed lid or a solid piece of foil on the lid. However, you are misrepresenting the core issue which is the failure to germinate, which has nothing to do with gas exchange.
|
the_0wl
The Space Bird
Registered: 04/15/08
Posts: 442
Last seen: 2 years, 9 months
|
Re: Anything look wrong with these rye jars? (pics) Not growing. . [Re: shroober]
#8475056 - 06/02/08 12:54 PM (3 years, 11 months ago) |
|
|
Yeah, I am a little puzzled by a total lack of germination. In one more week I guess I'll just roll again. Cant imagine it taking 3 weeks to even begin to see anything
--------------------
Everything I say on this website and forum is completely false and fantasy role play. In reality I do not engage in any illegal activities regardless of what my posts say.
|
|
Previous
Index
Next
Threaded
Edit 
Reply 
