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 BlimeyGrimey
Pyon!
Registered: 08/24/05
Posts: 911
Loc: Seattle, Wa
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 Puerto Rican Ganoderma Isolation! (Updated with better pics)
#8312940 - 04/22/08 12:56 AM (5 months, 19 days ago) |
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Some of you might remember my Ganoderma specimen I found in Puerto Rico.
After cutting a small cube of the inner pore tissue out, I swiped the cube across the center of a single PDA plate. 8 days later I had a single tiny white germination spot.
8 days after germination the plate looks like this.
EDIT: Are the thicker rhizo parts "sectors"? For some reason it looks to me as though there aren't any sectors. Atleast not what I've seen from other species. There are usually "lines" between the sectors.
I'll be transferring a piece of this plate to a MEA plate later tomorrow.
Seems I got lucky and had 0 contams and it even looks to be growing very evenly. Shouldn't be hard to get a few isolates from this culture.
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Confirmed:
Psilocybe stuntzii
Psilocybe cyanescens
Psilocybe guilartensis
Panaeolus olivaceus
Unknown:
Washington Psilocybe species
Washington Panaeolus species
Edited by BlimeyGrimey (04/23/08 03:25 PM)
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 Andrew47
Servant of allLife
Registered: 04/06/06
Posts: 390
Last seen: 20 days, 7 hours
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Re: Puerto Rican Ganoderma Isolation! [Re: BlimeyGrimey]
#8313968 - 04/22/08 11:01 AM (5 months, 19 days ago) |
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Is the mycelium the actual color shown in the picture? Ganoderma Lucium mycelium on agar is white, though it could be possible this Ganoderma looks like that. If it were me, though, I'd consider that a very possible contamination
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BlimeyGrimey
Pyon!
Registered: 08/24/05
Posts: 911
Loc: Seattle, Wa
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Re: Puerto Rican Ganoderma Isolation! [Re: Andrew47]
#8314950 - 04/22/08 03:44 PM (5 months, 19 days ago) |
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The culture is pure white, with a "papery" look to it. Hard to explain but the edges of the mycelium look like torn soft toilet paper.
The color you see in the picture is because it was placed against my monitor to backlight it.
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Confirmed:
Psilocybe stuntzii
Psilocybe cyanescens
Psilocybe guilartensis
Panaeolus olivaceus
Unknown:
Washington Psilocybe species
Washington Panaeolus species
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BlimeyGrimey
Pyon!
Registered: 08/24/05
Posts: 911
Loc: Seattle, Wa
Last seen: 28 seconds
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Re: Puerto Rican Ganoderma Isolation! [Re: Andrew47]
#8319362 - 04/23/08 03:39 PM (5 months, 18 days ago) |
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Close up of mycelium:
Backlight pic:
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Confirmed:
Psilocybe stuntzii
Psilocybe cyanescens
Psilocybe guilartensis
Panaeolus olivaceus
Unknown:
Washington Psilocybe species
Washington Panaeolus species
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Andrew47
Servant of allLife
Registered: 04/06/06
Posts: 390
Last seen: 20 days, 7 hours
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Re: Puerto Rican Ganoderma Isolation! [Re: BlimeyGrimey]
#8322360 - 04/24/08 09:08 AM (5 months, 17 days ago) |
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Looks like Ganoderma mycelium  Congrats, that's a pretty cool achievement, picking a mushroom in the tropics and cloning it back at home
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It's easy! Send your clean prints to www.fsrcanada.com
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BlimeyGrimey
Pyon!
Registered: 08/24/05
Posts: 911
Loc: Seattle, Wa
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Re: Puerto Rican Ganoderma Isolation! [Re: Andrew47]
#8326901 - 04/25/08 12:34 PM (5 months, 16 days ago) |
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Thanks!
Yea, I'm 100% its Ganoderma mycelium now. I opened the dish to transfer a piece to a MEA dish and I noticed the mycelium is starting to get that tan faded color to it like most Ganoderma species do.
I'm amazed that I got a clean culture with no contams on the very first try!!
I'll be throwing a wedge into some aspen shavings later once I get my PC to sterilze it. Then I'll spawn that to alder or something with more substance to it.
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Confirmed:
Psilocybe stuntzii
Psilocybe cyanescens
Psilocybe guilartensis
Panaeolus olivaceus
Unknown:
Washington Psilocybe species
Washington Panaeolus species
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BlimeyGrimey
Pyon!
Registered: 08/24/05
Posts: 911
Loc: Seattle, Wa
Last seen: 28 seconds
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Re: Puerto Rican Ganoderma Isolation! [Re: BlimeyGrimey]
#8406967 - 05/15/08 08:50 PM (4 months, 27 days ago) |
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The transfer to MEA is going well.
Top-Lit Pic:
Backlit Pic:
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Confirmed:
Psilocybe stuntzii
Psilocybe cyanescens
Psilocybe guilartensis
Panaeolus olivaceus
Unknown:
Washington Psilocybe species
Washington Panaeolus species
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BlimeyGrimey
Pyon!
Registered: 08/24/05
Posts: 911
Loc: Seattle, Wa
Last seen: 28 seconds
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Re: Puerto Rican Ganoderma Isolation! [Re: BlimeyGrimey]
#8449693 - 05/27/08 06:11 AM (4 months, 15 days ago) |
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New better pics of the specimen.
Pic 1
Pic 2 (You can see the cube I cut out to streak on agar)
Pic 3 (Notice the fine yellow edge)
Pic 4 (Close-up of the tubes)
The 2 culture plates have been refrigerated until I can devote some time to a fruiting attempt.
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Confirmed:
Psilocybe stuntzii
Psilocybe cyanescens
Psilocybe guilartensis
Panaeolus olivaceus
Unknown:
Washington Psilocybe species
Washington Panaeolus species
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Andrew47
Servant of allLife
Registered: 04/06/06
Posts: 390
Last seen: 20 days, 7 hours
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Re: Puerto Rican Ganoderma Isolation! [Re: BlimeyGrimey]
#8450483 - 05/27/08 12:57 PM (4 months, 15 days ago) |
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So was it fruiting from underground roots or did it form that antler off a tree?
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It's easy! Send your clean prints to www.fsrcanada.com
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BlimeyGrimey
Pyon!
Registered: 08/24/05
Posts: 911
Loc: Seattle, Wa
Last seen: 28 seconds
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Re: Puerto Rican Ganoderma Isolation! [Re: Andrew47]
#8451241 - 05/27/08 04:37 PM (4 months, 15 days ago) |
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It was growing out of the dirt right next to a small tree stump. At first it looked like a shelf fungi attached to the stump. When I pulled it loose I saw that the antler was actually growing out of the dirt , literally up against the stump. I'm assuming it was feeding off of the roots or a lower underground part of the stump.
Here's an extra pic of the original PDA plate which was streakled with the pore tissue. Its browing quite nicely in the fridge.
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Confirmed:
Psilocybe stuntzii
Psilocybe cyanescens
Psilocybe guilartensis
Panaeolus olivaceus
Unknown:
Washington Psilocybe species
Washington Panaeolus species
Edited by BlimeyGrimey (05/27/08 04:58 PM)
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Andrew47
Servant of allLife
Registered: 04/06/06
Posts: 390
Last seen: 20 days, 7 hours
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Re: Puerto Rican Ganoderma Isolation! [Re: BlimeyGrimey]
#8455075 - 05/28/08 11:38 AM (4 months, 14 days ago) |
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Congratulations on your success so far this is really cool. I've been lucky enough to now clone wild mushrooms as part of my job, but never from a foreign country! I'm surprised it made the travel so well. What did you store it in? Usually if left in warm bags for a few days they get bacteria-laden
So what are your plans from here? Sawdust, or maybe an outdoor bed? Wooden dowels?
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It's easy! Send your clean prints to www.fsrcanada.com
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BrandNewbie
Captain
Registered: 05/21/08
Posts: 1,476
Loc: U.S.A.
Last seen: 14 days, 14 hours
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 Re: Puerto Rican Ganoderma Isolation! (Updated with better pics) [Re: BlimeyGrimey]
#8455956 - 05/28/08 04:34 PM (4 months, 14 days ago) |
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That is sssssoooooo awesome! I guess you need to start making syringes...
... for all your good friends, buddy! 
I wonder if a spore supplier would be interested in buying spores from a collector of a strain they didn't have?
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Lord, save me from your followers.
The more you complain, the longer God lets you live.
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BlimeyGrimey
Pyon!
Registered: 08/24/05
Posts: 911
Loc: Seattle, Wa
Last seen: 28 seconds
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Re: Puerto Rican Ganoderma Isolation! (Updated with better pics) [Re: BrandNewbie]
#8460891 - 05/29/08 06:18 PM (4 months, 13 days ago) |
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I plan on starting some pf style cakes first simply because I still havent got a new PC yet. Here's a tip, never get rid of your small 8 qt. PC until AFTER you have gotten a new larger one. 
Once those are colonized I'll spawn it to some pasteurized sawdust and attempt a fruiting. I might wait until I get a PC though.
Once I get some new plates I'll start trading wedges to people who want to trade.
I've sent a sample plate of this culture to Workman for further study.
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Confirmed:
Psilocybe stuntzii
Psilocybe cyanescens
Psilocybe guilartensis
Panaeolus olivaceus
Unknown:
Washington Psilocybe species
Washington Panaeolus species
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 Senor_Hongos
Stranger
Registered: 05/25/08
Posts: 3,976
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Re: Puerto Rican Ganoderma Isolation! (Updated with better pics) [Re: BlimeyGrimey]
#8460921 - 05/29/08 06:29 PM (4 months, 13 days ago) |
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Nice job!
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Amanitas kill more people than all other mushrooms put together, so an ID of some to be eaten must be correct. An ID based on a photo on the Internets is not reliable enough to potentially risk your life on. ToxicMan
Beginner's Guide to Mushroom name pronunciation
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BlimeyGrimey
Pyon!
Registered: 08/24/05
Posts: 911
Loc: Seattle, Wa
Last seen: 28 seconds
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Re: Puerto Rican Ganoderma Isolation! [Re: Andrew47]
#8536222 - 06/18/08 05:02 AM (3 months, 24 days ago) |
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Well I inoculated a pint jar of barley about a week ago. The jar is now about 75% colonized.
Would spawning this to coco fiber (animal bedding type) work for fruiting or does it have to be sawdust/chips/dowels?
I plan on just spawning the barley and coco fiber at 50/50 ratio then casing with sterilized cactus soil or plain vermiculite. I'll be attempting to fruit out of the jar kinda (using a plastic container instead of jar).
Any suggestions would be appreciated!
I'll post a pic of the colonizing jar tomorrow.
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Confirmed:
Psilocybe stuntzii
Psilocybe cyanescens
Psilocybe guilartensis
Panaeolus olivaceus
Unknown:
Washington Psilocybe species
Washington Panaeolus species
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tripsis
biophile-misanthropist-doofer
Registered: 04/13/08
Posts: 3,902
Loc: Gondwana
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Re: Puerto Rican Ganoderma Isolation! [Re: BlimeyGrimey]
#8536227 - 06/18/08 05:05 AM (3 months, 24 days ago) |
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Quote:
RogerRabbit said:
Reishi will grow on coir, sawdust-woodchips, and even straw, but not as well.
RR
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Since we depend on an abundance of functioning ecosystems to cleanse our water, enrich our soil and manufacture the very air we breathe, biodiversity is clearly not an inheritance to be discarded carelessly - Edward O. Wilson 1992
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BlimeyGrimey
Pyon!
Registered: 08/24/05
Posts: 911
Loc: Seattle, Wa
Last seen: 28 seconds
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Re: Puerto Rican Ganoderma Isolation! [Re: tripsis]
#8539178 - 06/19/08 12:02 AM (3 months, 24 days ago) |
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Here's a pic of some of the jars that I recently made. Barley and popcorn were soaked in a dilute (50%) coffee solution for 24hrs then strained for 1 hr and pc'ed for 90 minutes.
Here's a pic of my first jar of the PR Ganoderma which is almost done colonizing. Only needed 1 shake 4 days after inoculation (6/9) with an agar wedge.
I'll update as soon as I spawn it to some coco fiber. Maybe in 2 or 3 days.
--------------------
Confirmed:
Psilocybe stuntzii
Psilocybe cyanescens
Psilocybe guilartensis
Panaeolus olivaceus
Unknown:
Washington Psilocybe species
Washington Panaeolus species
Edited by BlimeyGrimey (06/19/08 03:22 AM)
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tripsis
biophile-misanthropist-doofer
Registered: 04/13/08
Posts: 3,902
Loc: Gondwana
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Re: Puerto Rican Ganoderma Isolation! [Re: BlimeyGrimey]
#8539184 - 06/19/08 12:03 AM (3 months, 24 days ago) |
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Nice. This has been a very interesting growlog so far.
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Since we depend on an abundance of functioning ecosystems to cleanse our water, enrich our soil and manufacture the very air we breathe, biodiversity is clearly not an inheritance to be discarded carelessly - Edward O. Wilson 1992
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BlimeyGrimey
Pyon!
Registered: 08/24/05
Posts: 911
Loc: Seattle, Wa
Last seen: 28 seconds
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Re: Puerto Rican Ganoderma Isolation! [Re: tripsis]
#8557582 - 06/24/08 08:49 AM (3 months, 18 days ago) |
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I spawned the 3/4 pint of barley to 3/4 pint of coco fiber in a small gladware container on the 21st. As of this morning its mostly colonized except for a few parts on the very bottom.
I plan to case this with sterilized cactus soil in the next few days.
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Confirmed:
Psilocybe stuntzii
Psilocybe cyanescens
Psilocybe guilartensis
Panaeolus olivaceus
Unknown:
Washington Psilocybe species
Washington Panaeolus species
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BlimeyGrimey
Pyon!
Registered: 08/24/05
Posts: 911
Loc: Seattle, Wa
Last seen: 28 seconds
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Re: Puerto Rican Ganoderma Isolation! [Re: BlimeyGrimey]
#8579549 - 06/30/08 02:10 AM (3 months, 12 days ago) |
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When I cased this container I accidentally grabbed a sterilized jar of coir instead of cactus soil. Its colonizing this layer quite fast and I've already thrown it into a FC. I'll probably just let it fruit uncased once its done colonizing. The mycelium on the sides of the container are turning a yellow color as it ages.
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Confirmed:
Psilocybe stuntzii
Psilocybe cyanescens
Psilocybe guilartensis
Panaeolus olivaceus
Unknown:
Washington Psilocybe species
Washington Panaeolus species
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