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Jair
Blasphemous



Registered: 06/08/07
Posts: 794
Loc: East Coast
Last seen: 7 days, 13 hours
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My first 10cc's (updated with some newb ?'s)
#8389584 - 05/11/08 04:01 PM (3 months, 25 days ago) |
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This is my first grow, I have ordered a 10cc Ecuador spore syringe from sporeworks here.
My plan is to knock up 6, 1/2 pint jars using up 6cc's (1cc for each jar and 1/4 cc per hole). Then I will use 2cc's for a honey LC and 2cc's for a syrup LC. If I time everything correctly I could possibly have three different grows all going at the same time (a regular spore grow, a honey LC, and a syrup LC). I basically did this because I wanted to make sure I had back up plans and didnt waste all of the spores in case they got contaminated.
So please, throw in your .02 and give me some beginner's tips! 
Also, I would like everyone to check out the grow log I have started here.
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My Ecuadorian Grow Log
Edited by Jair (05/11/08 06:52 PM)
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grymmtymm
lostinthought


Registered: 03/29/08
Posts: 456
Loc: midwest, unfortunately
Last seen: 15 hours, 56 minutes
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Re: My first 10cc's [Re: Jair]
#8389602 - 05/11/08 04:10 PM (3 months, 25 days ago) |
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if you actually use an incubator for your jars(what kind, PF tek or grain?) instead of just sitting them in a box in your room you will find that germination and colonization will happen much quicker.....
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Jair
Blasphemous



Registered: 06/08/07
Posts: 794
Loc: East Coast
Last seen: 7 days, 13 hours
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Re: My first 10cc's [Re: grymmtymm]
#8389608 - 05/11/08 04:11 PM (3 months, 25 days ago) |
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I am using the PK tek, as I heard it is pretty simple for a first-timer. What kind of incubator are we talking? I'm sort of on a budget.
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My Ecuadorian Grow Log
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blood4blood
Sifting through Evidence


Registered: 04/25/07
Posts: 2,894
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Re: My first 10cc's [Re: Jair]
#8389622 - 05/11/08 04:13 PM (3 months, 25 days ago) |
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its sounds like a good plan. remember to be sterile with all of your procedures.
this is a good lc tek. i found it to be ALOT better than using syrup or honey. if you can get your hands on some dry malt. http://www.shroomery.org/forums/showflat.php/Cat/0/Number/7887928/page/0/fpart/1/vc/1/nt/2
here are some pics of my lc. this is what it should look like as it progress's. you dont want it to be cloudy or hazey




hope this helps
-------------------- An eye for an eye will make the world go blind.
HazeyRoms said about Jeetered: "Ohh I cry in my fruting chamber to keep the RH up."
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P.Menace
Contradicting the Status Quo




Registered: 11/17/07
Posts: 2,401
Loc: Let Me See Potato Salad!!...
Last seen: 10 seconds
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but just for the record you only need 1/4cc per LC for a good result... Save yourself some of those valuable spores in case you mess something up
-------------------- Psilocybe Cubensis Menace
http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace
SainT420 said: You gotta be a Man of your Word... Or you will be a Man of NO WORDS! 6 feet Under
Prisoner # 1 said: always remember, the stupid ones never realize they're stupid... BlackMatch456,lil_demented
Blutjager said: "The work for payoff ratio for pf tek is like driving 500 miles to work all day for minimum wage,. in other words ITS NOT WORTH IT "
roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.
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grymmtymm
lostinthought


Registered: 03/29/08
Posts: 456
Loc: midwest, unfortunately
Last seen: 15 hours, 56 minutes
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Re: My first 10cc's [Re: Jair]
#8389637 - 05/11/08 04:18 PM (3 months, 25 days ago) |
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i use a tub in tub incubator, but that will require you spend a little money and a little time, i love that thing, it's perfect
but you could use a cooler with a reptile pad in it, or heating pad. that's easier. just not as effective
pretty much anything that heats the ambient air within an enclosed space, just not too much heat.
or if you don't want to do that at least put them somewhere warm like by a heat vent or an appliance that puts out heat
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Jair
Blasphemous



Registered: 06/08/07
Posts: 794
Loc: East Coast
Last seen: 7 days, 13 hours
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Re: My first 10cc's [Re: grymmtymm]
#8389687 - 05/11/08 04:34 PM (3 months, 25 days ago) |
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All right, thanks for all the help guys. I was planning on using _Otto_'s LC tek but I will look around and consider other ones as well, especially that one you gave me blood4blood.
1) About how long does it take the LC to reach the point where I can use it to knock up jars? 2) Anyone have any experience with Ecuadorians? Any luck? 3) What precautions should I take for sterilization to prevent contams? 4) Does Wal-mart have BRF? Does BRF come as flower or will i have to get BR and make it into BRF? How much perlite should i buy? Verm? BRF?
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My Ecuadorian Grow Log
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grymmtymm
lostinthought


Registered: 03/29/08
Posts: 456
Loc: midwest, unfortunately
Last seen: 15 hours, 56 minutes
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Re: My first 10cc's [Re: Jair]
#8389699 - 05/11/08 04:38 PM (3 months, 25 days ago) |
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i don't think wal-mart carries BRF, but yours may because they stock items according to the demographic in that area.
but you can make it yourself too, just a blender or coffee grinder to grind up some brown rice grains..
clean everything that will be touching your spores, culture, substrates, clean the air, use a pressure cooker,
don't skimp on the pressure cooker, the most important piece of equipment you will ever own for this hobby...
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Jair
Blasphemous



Registered: 06/08/07
Posts: 794
Loc: East Coast
Last seen: 7 days, 13 hours
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Re: My first 10cc's [Re: grymmtymm]
#8389936 - 05/11/08 05:40 PM (3 months, 25 days ago) |
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Quote:
grymmtymm said: i don't think wal-mart carries BRF, but yours may because they stock items according to the demographic in that area.
but you can make it yourself too, just a blender or coffee grinder to grind up some brown rice grains..
clean everything that will be touching your spores, culture, substrates, clean the air, use a pressure cooker,
don't skimp on the pressure cooker, the most important piece of equipment you will ever own for this hobby...
Well I don't own a pressure cooker which is also why I was going to use the PF Tek. I was just hoping that steam sterilization would work. If I do end up grinding up my own BRF how in the world am I supposed to sterilize that?
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My Ecuadorian Grow Log
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Jair
Blasphemous



Registered: 06/08/07
Posts: 794
Loc: East Coast
Last seen: 7 days, 13 hours
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Re: My first 10cc's [Re: Jair]
#8390170 - 05/11/08 06:32 PM (3 months, 25 days ago) |
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Some more questions:
1) Will placing verm or brf in the oven burn it? Should this be done to sterilize it? 2) Will an alcohol rinsed rag be ok to clean the air around my injection area? 3) What can you use in the bottom of your kettle if you dont have enough jar lids? 4) Will masking tape work ok if you dont have any micropore tape? 5) Where should the jars be stored after inoculation? Like temperature wise, light wise, cleanliness wise, etc. 6) How important is glovebox while injecting?
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My Ecuadorian Grow Log
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HoleSnype
I love me some me.



Registered: 04/27/06
Posts: 3,634
Loc: Chicken Fried Nation
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Re: My first 10cc's [Re: Jair]
#8390242 - 05/11/08 06:53 PM (3 months, 25 days ago) |
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1) Will placing verm or brf in the oven burn it? Should this be done to sterilize it?
- No need, because you will mix them 1st, put them in a jar, and then sterilize them. I would not place BRF in the oven. LOL.
2) Will an alcohol rinsed rag be ok to clean the air around my injection area?
-How are you going to clean the air with a rag?
3) What can you use in the bottom of your kettle if you dont have enough jar lids?
- You can get creative. They even sell "racks" at the grocery store. Anything that will not melt, and will sufficiently support the jars, keeping them out of the water.
4) Will masking tape work ok if you dont have any micropore tape?
Masking tape is no good. It doesn't allow air exchange. Them jars need to breath a little, so your top verm layer doesn't get moist and funky.
5) Where should the jars be stored after inoculation? Like temperature wise, light wise, cleanliness wise, etc.
Store the jars at room temp. I usually put mine in a dark place, but some don't think it is necessary, because FAE is the primary pinning trigger. Keep your jars in a place that is somewhat clean. Cleanliness is your primary concern during inoculation. (find a small room, and whipe everything down with bleach water. Shut the A/C vent so no air is blowing around, or use a glovebox.)
6) How important is glovebox while injecting?
They are pretty important. You could buy a tote for 5-10 bucks and make a glovebox. It is so simple, you might as well do it. The first time you have a bunch of green cakes, you will wish you had spent the 5 bucks.
Hey, just a heads up, you can find almost all of this information in the search engine.
-------------------- I'm sofa king we todd did.
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Jair
Blasphemous



Registered: 06/08/07
Posts: 794
Loc: East Coast
Last seen: 7 days, 13 hours
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Re: My first 10cc's [Re: HoleSnype]
#8390258 - 05/11/08 06:56 PM (3 months, 25 days ago) |
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Quote:
HoleSnype said: 1) Will placing verm or brf in the oven burn it? Should this be done to sterilize it?
- No need, because you will mix them 1st, put them in a jar, and then sterilize them. I would not place BRF in the oven. LOL.
2) Will an alcohol rinsed rag be ok to clean the air around my injection area?
-How are you going to clean the air with a rag?
3) What can you use in the bottom of your kettle if you dont have enough jar lids?
- You can get creative. They even sell "racks" at the grocery store. Anything that will not melt, and will sufficiently support the jars, keeping them out of the water.
4) Will masking tape work ok if you dont have any micropore tape?
Masking tape is no good. It doesn't allow air exchange. Them jars need to breath a little, so your top verm layer doesn't get moist and funky.
5) Where should the jars be stored after inoculation? Like temperature wise, light wise, cleanliness wise, etc.
Store the jars at room temp. I usually put mine in a dark place, but some don't think it is necessary, because FAE is the primary pinning trigger. Keep your jars in a place that is somewhat clean. Cleanliness is your primary concern during inoculation. (find a small room, and whipe everything down with bleach water. Shut the A/C vent so no air is blowing around, or use a glovebox.)
6) How important is glovebox while injecting?
They are pretty important. You could buy a tote for 5-10 bucks and make a glovebox. It is so simple, you might as well do it. The first time you have a bunch of green cakes, you will wish you had spent the 5 bucks.
Hey, just a heads up, you can find almost all of this information in the search engine.
Ok, thank you for all of the help. LOL, I didn't even think about what I was saying- flower in the oven... oh man I'm slow.
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My Ecuadorian Grow Log
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grymmtymm
lostinthought


Registered: 03/29/08
Posts: 456
Loc: midwest, unfortunately
Last seen: 15 hours, 56 minutes
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Re: My first 10cc's [Re: Jair]
#8390391 - 05/11/08 07:40 PM (3 months, 25 days ago) |
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Quote:
Jair said:
Quote:
grymmtymm said: i don't think wal-mart carries BRF, but yours may because they stock items according to the demographic in that area.
but you can make it yourself too, just a blender or coffee grinder to grind up some brown rice grains..
clean everything that will be touching your spores, culture, substrates, clean the air, use a pressure cooker,
don't skimp on the pressure cooker, the most important piece of equipment you will ever own for this hobby...
Well I don't own a pressure cooker which is also why I was going to use the PF Tek. I was just hoping that steam sterilization would work. If I do end up grinding up my own BRF how in the world am I supposed to sterilize that?
you wouldn't need to sterilize it seperately, just use it just like you bought it at the store, mix it up, jar it, then sterilize it with your chosen sterilizing method..
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Jair
Blasphemous



Registered: 06/08/07
Posts: 794
Loc: East Coast
Last seen: 7 days, 13 hours
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Re: My first 10cc's [Re: grymmtymm]
#8397752 - 05/13/08 05:43 PM (3 months, 23 days ago) |
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Ok a couple more questions.
1) I've seen in a grow log before that some people actually have little pin heads starting to grow in their myc before they even take the cakes out of the jars to dunk and roll, should I wait to see this? Does this usually happen or rarely?
2) I read somewhere that cakes will sometimes have 2,3, and even 4 flushes, should I leave my cakes out and see if they will continue to grow or just throw them out after I pick my first flush?
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My Ecuadorian Grow Log
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grymmtymm
lostinthought


Registered: 03/29/08
Posts: 456
Loc: midwest, unfortunately
Last seen: 15 hours, 56 minutes
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Re: My first 10cc's [Re: Jair]
#8398064 - 05/13/08 07:21 PM (3 months, 23 days ago) |
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1. no, you do not have to wait for this to happen. when you are ready to fruit, just go ahead and do the soak. does pinheads forming rarely happen? that always happens, the pinheads are the baby mushrooms in their first stages of growth, without pinning, you won't have mushrooms.
2.definitely, leave them to grow as many flushes as you can get. when you have your first flush or right before it, would be a good time to start some more cakes if you have enough jars and syringes, thereby, by the time your fist set of cakes are done fruiting, your second set will be close to fruiting...
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