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 Re: Black whisker mold [Re: Lutioush]
#8331981 - 04/26/08 08:00 PM (5 months, 12 days ago) |
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I'd definately reccomend that you put a HEPA unit in the room where you do your glovebox for an hour before you do your work in it. Are you sure your alcohol wiping your glovebox and everything else before putting it in the glovebox? I can remember RR once saying you need to get the spot under the lid in order to make sure it's not contaminated.
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"Who said every wish would be heard and answered when wished on a morning star? Some asshole thought of it, some sucker believed it and look what it's done so far." -the great LoC
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Lutioush
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Yeah a hepa would be good but it's going to be a while before i can afford one. I've just started some more jars today. I've got three different sources and each set has three jars. I've got two different spore syringes made from separate prints and a apple juice LC that looked good. We'll see what happens.
I did have a couple questions. What is a good colonizing temp for grain. I've got my incubator at about 83 right now.
Also, how good is the tyvek at filtering. If one of my jars in the incubator gets infected is there any chance of it spreading to the other jars. I have it double layered but i was wondering if it was an absolute filter or just a semi barrier. Just wondering I guess
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Re: Black whisker mold [Re: Lutioush]
#8336189 - 04/27/08 11:33 PM (5 months, 11 days ago) |
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Yea there's your problem 75 is ideal for incubation because the jars themselves create heat while growing so internal temp is about 80. This could definitely be your problem. higher temps help the contams grow and hinder mycilium.
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"Who said every wish would be heard and answered when wished on a morning star? Some asshole thought of it, some sucker believed it and look what it's done so far." -the great LoC
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Lutioush
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Re: Black whisker mold [Re: Lutioush]
#8336218 - 04/27/08 11:41 PM (5 months, 11 days ago) |
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well crap
I suppose i should have connected the two when I started having problems when i built the incubator. Well... time to go adjust my tank heater
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Lutioush
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Re: Black whisker mold [Re: Lutioush]
#8344930 - 04/30/08 03:26 AM (5 months, 8 days ago) |
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I figured I'd give an update since it's been a few days since I've knocked up my jars. Still no signs of colonizing but at the same time no signs of contams (IE no smells or visual identifiers). The three biggest things I changed were temps (78ish), grain wetness, and inoculation method. I'll post more in a couple of days.
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Lutioush
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Re: Black whisker mold [Re: Lutioush]
#8346956 - 04/30/08 06:46 PM (5 months, 8 days ago) |
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Ok update. I'm making this my official contam log.
A. 3 half pint jars were knocked up using Syringe 1 containing spores from a print i made a long time ago. I made this one a day before I knocked up that jars in a glove box with extremely sterilized conditions (as best as i could get them).
All jars still look fine. No signs of contams whatsoever. No
signs of growth.
B. 2 half pint jars and one quart jar were knocked up with an apple juice LC syringe that I made several months back and was one of the three that didn't show any signs of contams. I made the syringe about an hour before knocking up the jars.
All jars have started showing signs of growth except for the quart jar and this is probably due to size. It is simply small dots of mycelium but it's still growth. This one is expectedly faster then the other sets simply because it was a well colonized LC
C. 3 half pint jars made from a spore syringe that has been sitting in my fridge for about two months. This is the black sheep of the groups because I discovered cobweb accumulating in all jars and was forced to toss them. I somewhat expected this one to go on my simply due to the fact that it was an old syringe.
So far my only contams have been that cobweb in the three jars and I've isolated that down to the syringe. I will keep posted on the other 6 jars.
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Re: Black whisker mold [Re: Lutioush]
#8347886 - 04/30/08 10:49 PM (5 months, 8 days ago) |
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good luck
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"Who said every wish would be heard and answered when wished on a morning star? Some asshole thought of it, some sucker believed it and look what it's done so far." -the great LoC
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Lutioush
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Re: Black whisker mold [Re: Lutioush]
#8348484 - 05/01/08 03:16 AM (5 months, 7 days ago) |
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quick question about colonization times. How long should you wait to assure that the LC or the spores aren't contamed? Should I wait till the whole jar is colonized or half? Is there any certain time period or just go with full colonized?
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Re: Black whisker mold [Re: Lutioush]
#8351259 - 05/01/08 08:13 PM (5 months, 7 days ago) |
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I'm not entirely sure what your asking, but you want whole colonization before you use it anyway. A lot of times mold starts white and becomes green or whatever else afterward. So usually you should not be able to tell until it's fully colonized. However if you spot a problem before near full colonization you should probibly just toss it since it's not likely to recover.
Out of curiosity though are you sure that the cobweb wasn't healthy mycilium? Cause I've seen people mistake healthy mycilium for cobweb a few times on here.
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"Who said every wish would be heard and answered when wished on a morning star? Some asshole thought of it, some sucker believed it and look what it's done so far." -the great LoC
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Lutioush
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My question was how long it would be before I knew that my LC wasn't contamed from the jars that I already knocked up.
As for the cobweb I've had to before (I think haha) but my indicators are that it colonized half the jar in about a day and it was a little darker and more wispy then actual myco
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Lutioush
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Re: Black whisker mold [Re: Lutioush]
#8392998 - 05/12/08 02:26 PM (4 months, 27 days ago) |
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I'm going to go with problem solved. I'm pretty sure it was simply the wetness in the jars. Out of twenty jars I lost two and those were both to bacteria. I'm going to assume I fixed it. Other then jar wetness I also started using the oust stuff in the air. Thanks for the help guys
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Lutioush
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Re: Black whisker mold [Re: Lutioush]
#8479785 - 06/03/08 02:48 PM (4 months, 5 days ago) |
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I'm not only going to do LC because most of the last batch which i thought were fine actually ended up contaming (mostly bacteria). I knocked up a whole bunch of pint jars a while back with LC and had 100% success ratio so I figure I'll just do that on quart jars.
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