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 didjin_d
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 Powdery look to oyster myc...normal?
#8383394 - 05/09/08 09:47 PM (3 months, 19 days ago) |
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I started with a sporeprint, then put in water syringe, then onto agar. After getting a clean sample I inocculated 4 jars of grain.
All 4 jars of oyster mycelium, have this powdery look where the myc meets the glass (see pic). Is this normal for oysters jars? (agar transfer on 4/26, pics taken 5/9)
2 jars were shaken on 5/7, and these no longer seem to have the powdery stuff.
Thanks for the help,
-DD
Edited by didjin_d (05/10/08 04:09 PM)
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psilociety
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Re: Powdery look to oyster myc...normal? [Re: didjin_d]
#8383613 - 05/09/08 10:49 PM (3 months, 19 days ago) |
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looks great. now you have oyster spawn, congrats.
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psilociety
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Re: Powdery look to oyster myc...normal? [Re: psilociety]
#8383644 - 05/09/08 10:57 PM (3 months, 19 days ago) |
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here is a jar of golden oyster. it is a little dry for being only 50% colonized though, it will do.
sorry about shitty computer camera...
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didjin_d
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Re: Powdery look to oyster myc...normal? [Re: psilociety]
#8385936 - 05/10/08 04:06 PM (3 months, 18 days ago) |
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Thanks for the encouragement psilociety!
Anyone else with oyster experience want to chime in?
-DD
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-DD
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Cryogenicz
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Re: Powdery look to oyster myc...normal? [Re: didjin_d]
#8387653 - 05/11/08 12:09 AM (3 months, 18 days ago) |
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That does not look very good to me.. What variety is it?
-Graham
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didjin_d
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Re: Powdery look to oyster myc...normal? [Re: Cryogenicz]
#8389322 - 05/11/08 02:32 PM (3 months, 17 days ago) |
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The spores are from a kit I got, the maker called them "blue oysters".
Here are two jars that were shaken on the 7th (pics taken on 11th) mentioned in initial post. Guess the powdery appearance is not all gone.
The jar in the first pic was ~90% colonized when shaken, the second jar was ~50-75% colonized when shaken.
It seems like most contaminates are colorful, and fairly obvious once they create spores. Are there many white contaminates out there?
I'm going to inoculate some pasteurized hay. But if the powdery stuff is indeed powdery (puffs into air like spores), I'll probably just scrap it. Unless someone out there thinks the oysters would out-compete whatever may be in there and still be safe to eat(?).
Thanks
-DD
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-DD
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makaveli8x8
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Re: Powdery look to oyster myc...normal? [Re: didjin_d]
#8391329 - 05/12/08 12:57 AM (3 months, 17 days ago) |
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ive seen that before, i can't remember what happened with it but i "think" your ok and its normal
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Cryogenicz
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Re: Powdery look to oyster myc...normal? [Re: makaveli8x8]
#8392014 - 05/12/08 09:39 AM (3 months, 17 days ago) |
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If I started off with spores, and I saw that kind of growth, I would defiantly isolate away from it.. It could be ok, but my personal gut instinct tells me it looks bad. Yeast can take on a white/tan colored powdery look, so thats why I'm saying it doesn't look like pure mushroom mycelium.
-Graham
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 UrbanFungi
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Re: Powdery look to oyster myc...normal? [Re: Cryogenicz]
#8392522 - 05/12/08 12:18 PM (3 months, 16 days ago) |
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Don't use hay, use straw. Hay is green and straw is golden. If you were to use hay, it would begin to "hot compost" as soon as you moistened and spawned it.
I think you should go ahead and use it, but if it doesn't work out go to sporeworks and buy a pure culture syringe. Use it to make your own liquid culture so that you have a useful amount of inoculum.
I always make more spawn than necessary and that looks like spawn I would rather feed to my goats. It doesn't show concrete evidence of contamination but it is uneven and that's a red flag.
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didjin_d
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Re: Powdery look to oyster myc...normal? [Re: UrbanFungi]
#8395028 - 05/12/08 11:45 PM (3 months, 16 days ago) |
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Graham,
Didn't see any unusual growth in the plate, I didn't notice the powdery look until the mycelium pressed against the glass in the grain jars.
Being my first experience growing oysters from spores, I'm gonna give it a try anyway. Might as well.
Thanks for the correction Urban, I meant to say straw, not hay. Tomorrow I'm going to inoculate some extra straw from a garden-mushroom patch I just started (cross my fingers).
I'm going low-tek and using a peroxide bath to treat the straw. Not the best, but the mycelium is rising well toward the jar lids, and I'm not going to have time to pasteurize for a while.
Thanks to everyone for the feedback! Keep it coming if you have more... and I'll keep you posted on the results.
-DD
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FreeSporePrints
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Re: Powdery look to oyster myc...normal? [Re: didjin_d]
#8395575 - 05/13/08 06:07 AM (3 months, 16 days ago) |
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It looks me a contamination, or better mycelium and a white mold.
You should smell the spawn when you'll inocule the fruting substrate.
The reason can be 2:
1) you didn't clean at 100% the mycelium, so you transferred with it that white mold.
2) the sterilization of the jars wasn't sufficient, but i'd exclude this reason, because in my little experience i've seen just green or coloured molds on grains.
it COULD be that the mycelium is quite sane, generally in my experience it happens when the kernels are too dry or if as in the first reason, you've a contaminant.
The only thing i can suggest to you to be sure is to take a kernel and inocule a petri plate, then see what happens.
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