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Offlineamanitavirosa
absurdlyoptimistic
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Registered: 04/07/08
Posts: 430
Loc: Cold places
Last seen: 14 years, 9 months
$40 glove box - a must
    #8322355 - 04/24/08 09:06 AM (15 years, 11 months ago)



So this is the completed box - roughly how you would see it as you worked.


Nice large opening to place plenty of items in for sterile work. The only "issue" is that you can barely touch the far-wall with the gloves, making manipulating things in the far corners interesting.


$1.99 computer fans wired in parallel push a decent amount of air through the filter!


Here you can see the HEPA filter ($20) is glued to the side of the glove box using hot-glue gun adhesive. Surrounding the filter element is a dollar store tupperware container. To the outside of the tupperware container, you see the two computer fans.


The whole assembly is further mechanically supported by a few ty-wraps (cable ties).


The glue gun allows for a very quick air-tight seal. If you are patient, silicone will work just dandy too. Glue sticks are super cheap though.


$6 gloves purchased at a farm supply-store glued into a $9 storage bin.

Total cost: $39.98
Total value: priceless

This box has allowed countless liters of grain-to-grain transfer, spore printing, cloning to agar, etc. with no contamination to date.


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-AV-

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Invisiblebigcraig77
Registered: 11/09/07
Posts: 106
Re: $40 glove box - a must [Re: amanitavirosa]
    #8322372 - 04/24/08 09:11 AM (15 years, 11 months ago)

nice setup man. ive got a ghetto setup. mines a toaster oven box wrapped in cling wrap. i spray the inside with lysol and clean my hands and arms really good. no contams to date.

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InvisibleBlutjager
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Registered: 06/11/06
Posts: 9,220
Re: $40 glove box - a must [Re: bigcraig77]
    #8322380 - 04/24/08 09:13 AM (15 years, 11 months ago)

Good work,I personally would never have went through the trouble of making it but I sure as hell like the looks of it,5 for you :rockon:

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Offlineamanitavirosa
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Registered: 04/07/08
Posts: 430
Loc: Cold places
Last seen: 14 years, 9 months
Re: $40 glove box - a must [Re: Blutjager]
    #8322404 - 04/24/08 09:20 AM (15 years, 11 months ago)

My problem with this hobby is that I enjoy building too much. If only I had more space .. lol ..

My next project is a covert all-in-one tool-box FC.


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-AV-

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Invisiblevirus1824
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Registered: 09/25/05
Posts: 1,751
Loc: Europe
Re: $40 glove box - a must [Re: amanitavirosa]
    #8322418 - 04/24/08 09:24 AM (15 years, 11 months ago)

may I ask how you cut the holes for the gloves?


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A weekend wasted is never a wasted weekend

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Offlinegrod31
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Registered: 12/06/07
Posts: 2,077
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Re: $40 glove box - a must [Re: virus1824]
    #8322558 - 04/24/08 10:01 AM (15 years, 11 months ago)

the easiest way to cut holes in a glove box is to get a metal coffee can and leave it on your stove until hot. then place the open end on your glove box were you want your holes, dont inhale the black smoke.
wallah two perfect holes.


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Back the tape up.  I need it again!
Let it roll!  Just as high as the
fucker can go!  And when it comes
to that fantastic note where the
rabbit bites its own head off, I
want you to  THROW THAT FUCKING
RADIO INTO THE TUB WITH ME!
    Not me.  It would blast you through
the wall stone dead in ten
seconds and they'd make me explain
VVVVV Free Myco Thread

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InvisibleNariusFractal
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Registered: 12/19/02
Posts: 804
Loc: USA
Re: $40 glove box - a must [Re: grod31]
    #8322661 - 04/24/08 10:42 AM (15 years, 11 months ago)

Thanks for that nice tip! Very creative and effective solution.


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You are the microcosm of the macrocosm.

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Invisiblevirus1824
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Registered: 09/25/05
Posts: 1,751
Loc: Europe
Re: $40 glove box - a must [Re: grod31]
    #8322693 - 04/24/08 10:56 AM (15 years, 11 months ago)

Perfect! thank you for that very nice tip indeed.

I tried it with a drill and alot of holes. You can already guess that was quite time consuming.


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A weekend wasted is never a wasted weekend

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OfflinedeXtrous
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Registered: 04/24/06
Posts: 1,743
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Re: $40 glove box - a must [Re: virus1824]
    #8322711 - 04/24/08 11:05 AM (15 years, 11 months ago)

here's my glovebox... simple, no air filtration.







yours is way better though :laugh: mines kinda hard to reach and stuff..

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OfflineAkira
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Registered: 12/30/05
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Re: $40 glove box - a must [Re: deXtrous]
    #8322763 - 04/24/08 11:20 AM (15 years, 11 months ago)

I always find that with attatching gloves to the GB it is always hard to reach the far corners of the GB. But if you make the gloves too long (like with using insimination gloves)then you just have a bunch of excess glove material laying all over your work area.

I usually do 12 jar G2G's at a time, and so I need plenty of space. I need to build a flowhood i think...

Anyways nice job. But why the filter and fan? From whaty I understand a GB is supposed to be still air. Fans defeat the purpose. Not to mention the $20 HEPA filters dont really filter 99% airborn bacteria like the other ones, so it may bring in contams into your clean area..


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Orissa India Bulk Grow (Tub Tek)
Bulk Steamer Pasteurizer Tek

"Our intention is our eternal fingerprint in the universe."

We know that God is good, and so are hamburgers and hot dogs. We know that hamburgers and hot dogs definitely do exist, so then by deduction of logic God too must also exist. Hamburgers + Hot dogs = God.... Duh

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Offlineoriginal1986
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Registered: 03/12/08
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Re: $40 glove box - a must [Re: deXtrous]
    #8322792 - 04/24/08 11:27 AM (15 years, 11 months ago)

yeah its a good set up but the gloves are a Lil to much i just cut holes about 1.5 inches bigger than my arms and used arm guards to fill the gaps and wahlah no air gets through and i can reach all around i just spray my arms with Lysol before i reach in an i dont flame sterilize i wipe real good with alcohol so i dont have to pull my arms out but your set up is nice though.


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I would like to thank all the people who contribute there knowledge on this site which helps the average person like my self take up this hobbie.I see no harm in growing for personal use ill never grow enough to get a serious charge and i have a pretty good lawyer.

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OfflinedeXtrous
complete tool
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Registered: 04/24/06
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Re: $40 glove box - a must [Re: Akira]
    #8322818 - 04/24/08 11:32 AM (15 years, 11 months ago)

Where the hepa filter is attached to the inside of the container you should cover with tyvek if you wanted an extra precaution agaisnt bacteria (and have a totally badass setup)

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Offlinemephist00
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Registered: 04/15/08
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Re: $40 glove box - a must [Re: deXtrous]
    #8322852 - 04/24/08 11:43 AM (15 years, 11 months ago)

yea nice looking box

i just used a rubbermaid container and put 2 holes for my arms, im using disposable horse butt gloves lol with a ring at the end to when my arms get far enough in, it creates a seal.

i like the hole covering idea u have.. i think i might implement that into my design :>


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"feed your head!!"

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Offlineamanitavirosa
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Registered: 04/07/08
Posts: 430
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Re: $40 glove box - a must [Re: deXtrous]
    #8322887 - 04/24/08 11:54 AM (15 years, 11 months ago)

Guys, HEPA is pretty much the best filter you can use. No bacteria will get through - and if they do, they are < 0.3 microns in size.

Tyvek will not do any better.


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-AV-

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InvisibleBrainiac
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Registered: 04/29/06
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Re: $40 glove box - a must [Re: amanitavirosa]
    #8322909 - 04/24/08 12:02 PM (15 years, 11 months ago)

Quote:

amanitavirosa said:
Guys, HEPA is pretty much the best filter you can use. No bacteria will get through - and if they do, they are < 0.3 microns in size.

Tyvek will not do any better.




If its a true hepa..Not like the ones, you buy at Wal-mart...


--------------------
:Awesketch:

:cool: Fair is Fair :devil:

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InvisibleFooMan
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Re: $40 glove box - a must [Re: Brainiac]
    #8322920 - 04/24/08 12:04 PM (15 years, 11 months ago)

Yep, usually $20 HEPA's don't filter down to .3 microns.


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Quick WBS Prep

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Offlineamanitavirosa
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Registered: 04/07/08
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Re: $40 glove box - a must [Re: FooMan]
    #8322934 - 04/24/08 12:11 PM (15 years, 11 months ago)

No - its a true HEPA brand filter .. the reason it is only $20 is because of its small size. On the box, It even described the particle size that it filters to.

For those who are wondering, the HEPA filter and fan combo is to provide sterile air to create positive pressure. This allows me to douse the chamber with disinfectant and then thoroughly air it out so that I can flame scalpels, etc.

The positive pressure provides a truly clean environment.


--------------------
-AV-

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OfflineOphanim
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Registered: 10/01/07
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Re: $40 glove box - a must [Re: amanitavirosa]
    #8322990 - 04/24/08 12:29 PM (15 years, 11 months ago)

Wicked box. What do you wire the computer fans to in order to power them?

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InvisibleBrainiac
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Re: $40 glove box - a must [Re: amanitavirosa]
    #8323109 - 04/24/08 01:16 PM (15 years, 11 months ago)

Quote:

amanitavirosa said:
No - its a true HEPA brand filter .. the reason it is only $20 is because of its small size. On the box, It even described the particle size that it filters to.

For those who are wondering, the HEPA filter and fan combo is to provide sterile air to create positive pressure. This allows me to douse the chamber with disinfectant and then thoroughly air it out so that I can flame scalpels, etc.

The positive pressure provides a truly clean environment.




Like they never lie.... take a laser particle counter to it and see what it is...But for must stuff it wont matter..


--------------------
:Awesketch:

:cool: Fair is Fair :devil:

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Offlineamanitavirosa
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Registered: 04/07/08
Posts: 430
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Re: $40 glove box - a must [Re: Brainiac]
    #8323351 - 04/24/08 02:45 PM (15 years, 11 months ago)

Quote:

Ophanim said:
Wicked box. What do you wire the computer fans to in order to power them?




I am using a 110v transformer that outputs 12V at 400mw (a cordless drill charger plug actually). It spins the fans just nicely. Decent positive pressure to the point where its not blowing things around in the box and exchanges the air once every 3-4 mins.

Quote:

Brainiac said:Like they never lie.... take a laser particle counter to it and see what it is...But for must stuff it wont matter..




I'll get right on that...

When I made the glovebox, I did a spore capture-experiement that consisted of exposing a number of petri dishes with various nutrient agar formulations to the filtered air for an hour. The formulae used were: PDA (potato dextrose agar), MEA (malt extract agar), LB (Lennox Broth [bacterial]), and sheep blood (pathogen capture anybody?).

To really test the box, I disturbed the surrounding air as much as possible by pointing a fan directly at the inlet port and vacuuming the apartment (ancient vacuum with crappy filter element).

0.0% contamination on first exposure, 0.0% contamination on second over-night exposure when incubated for 72h.

Its a HEPA.

Besides, if the HEPA brand wanted to rip people off by selling sub-standard products, they are liable to many lawsuits. I sincerely doubt that they would risk such a thing. Besides, even if they did .. I bet the filter is still far better than most of your aseptic techniques :smirk:.


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-AV-

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