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 Lutioush
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 Black whisker mold
#8265630 - 04/10/08 06:17 PM (4 months, 16 days ago) |
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Quick question about this. Is it introduced through the or is it like an endospore that survives the heating process. I've been having problems on my last run with this one and Bacillus but I was only PCing my jars for 45 minutes. I've started going for 90 min instead. Will this help or is it a sanitation issue after the jars are PCed?
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Lutioush
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Re: Black whisker mold [Re: Lutioush]
#8286758 - 04/15/08 12:42 PM (4 months, 11 days ago) |
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bump. I'm still trying to figure out if this is is introduced from the rye berries not being pced long enough for if it is introduced more often. Any ideas?
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 ScavengerType
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Re: Black whisker mold [Re: Lutioush]
#8298555 - 04/17/08 09:55 PM (4 months, 9 days ago) |
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usually people PC for a minimum of 60 min
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"Who said every wish would be heard and answered when wished on a morning star? Some asshole thought of it, some sucker believed it and look what it's done so far." -the great LoC
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Lutioush
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I know what typical PC times are but i can't seem to shake this stuff. I've had jars that are just about entire colonized and then this stuff kicks in and spreads and i have to toss the whole thing. I just can't figure out where it's coming from.
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Re: Black whisker mold [Re: Lutioush]
#8302086 - 04/18/08 06:37 PM (4 months, 8 days ago) |
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90 min is a safe and recommended PCing time 60 is considered the minimum. If you were PCing for only 45 then obviously this could be the problem. How's the 90min jars working for ya?
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"Who said every wish would be heard and answered when wished on a morning star? Some asshole thought of it, some sucker believed it and look what it's done so far." -the great LoC
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poof
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I always shoot for 90-120 minutes, where 60 has always contam'd.
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Lutioush
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Re: Black whisker mold [Re: poof]
#8307647 - 04/20/08 11:44 AM (4 months, 6 days ago) |
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Out of all the 90 min jars I had 2 that got black whisker and one that got Bacillus out of 7. I just tried two hours and we'll see how that goes. It always seems to develop near the end of colonization. I'll update when these jars get farther along
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Lutioush
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Re: Black whisker mold [Re: Lutioush]
#8313290 - 04/22/08 03:41 AM (4 months, 4 days ago) |
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Well I've already had 1 jar go to black whisker and 2 too Bacillus. I think I've pretty much isolated that it isn't my PC time because 2 hours should kill pretty much anything. I suppose the next step will be to wipe down every inch of my glove box with alcohol. I've been using lysol up until now so we'll see if this helps any.
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Re: Black whisker mold [Re: Lutioush]
#8314681 - 04/22/08 02:44 PM (4 months, 4 days ago) |
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Yea Make sure your sterile techniques are up to par. Though a high moisture content can also invite contaminants. I just lost 18 jars to having too high of moisture content.
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"Who said every wish would be heard and answered when wished on a morning star? Some asshole thought of it, some sucker believed it and look what it's done so far." -the great LoC
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Lutioush
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Alright I just did 7 jars with the following parameters.
The original jar was fully colonized about a week ago to ensure that nothing suddenly appeared (Black Whisker, Bacillus)
Grain jars were PCed at 15 psi for one hour
Glove box was wiped down with rag soaked in 70 % alcohol
Jars were also wiped down with rag of alcohol before being placed in glove box
Vinyl gloves were used and sanitized with 62% alcohol hand sanitizer.
I can't think of any better way to sanitize it then this with my current resources (I.E. lack of flow hood) One other thing I changed was that I added 1 tablespoon of Gypsum for the first time. As far as I can tell it really doesn't have any effect on contams but is simply to help break up the grains.
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Re: Black whisker mold [Re: Lutioush]
#8319023 - 04/23/08 02:22 PM (4 months, 3 days ago) |
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Are you washing that rag between uses?
otherwise I'd ask is your soundings heavily moldy?
And you did not listen to well Grain jars are supposed to be PCed 60min-90min. It shouldn't be much of a problem (especially with G2G) but I assumed you'd likely do 90 given your circumstances.
Out of curiosity what method are you using to prepare the grains and get the right moisture content?
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"Who said every wish would be heard and answered when wished on a morning star? Some asshole thought of it, some sucker believed it and look what it's done so far." -the great LoC
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Lutioush
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The rag was washed between uses and it is probably true that I should have done the max of 90 min but since 2 hours didn't seem to solve the problem I'm assuming my issues aren't in how long I PC but elsewhere.
As for the grain prep method I've been simmering it for 45 min and then letting it steam dry for about 15 in a strainer. It comes out with a relatively dry surface area.
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 preschooler
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Re: Black whisker mold [Re: Lutioush]
#8319661 - 04/23/08 04:52 PM (4 months, 3 days ago) |
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if your using a 7 quart jar pc...... if you do four jars 60 min is fine....any more and you need atleast 90 min.... this is not in any book or anything just my experiences.... what kind of lid are you using? Tyvek or polyfill?
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Lutioush
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double layered tyvek envelopes from the post office.
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Lutioush
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Re: Black whisker mold [Re: Lutioush]
#8325635 - 04/25/08 01:07 AM (4 months, 1 day ago) |
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So far I've lost 2 jars to trich out of the new batch. I think I might move by glove box to a different room and hope that the air there is a bit more sanitary. I don't have a hepa filter and I was previously doing it in the basement but I figured that since it was a alcohol sanitized glove box it would be alright. I guess not though. Anyone ever tried that oust air sanitizer stuff? I might try that.
Another option would be to go OCD on the room a few times with the alcohol. I figure if I wipe it all down tonight and then give the majority of the stuff in the air to settle and then rewipe it down tomorrow night that might help. I've never really had this many problems with grains but lately it seems to be kicking my ass.
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preschooler
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Re: Black whisker mold [Re: Lutioush]
#8326257 - 04/25/08 08:38 AM (4 months, 1 day ago) |
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i think you should look more into ur procedure ..... here's a some questions for ya
are you sure your spores are viable?
what are you innoculating with?
how are you preparing your grain?
if i was having grain problems like this i would simmer or steap my wbs for 45 min and drain then rinse and drain for 20 min. then load....and pc for 90 min.... also i would look into using some poly fill in between two filters... are you cutting circles or anything with the tyvek lids.... or just strapin it to the top?
i also would look into doing a g2g of a good jar... if you got one.... find the brightest white.. fastest colonizer of the bunch
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Just want to give my thanks to the shroomery. You guys have made it possible for me to see the world in a different light so to speak. I do believe it is getting better every year that i am here and i believe what you are doing is good for the new and older members. Its also very cool to see an orginazation that gives drugs and psychotropic plants the respect they deserve. keep it up!!!!!
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Lutioush
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I just started three new jars with spores so we'll see how those go. I think my problem is going to end up being i'm not drying my grains to enough.
I added a tablespoon of gypsum to each jar. Is that to much? Am I even supposed to add it to the jars or should i just add it to the soaking water when i'm soaking for endospores. I've read both so I was wondering. I'm going to go buy a few strainers and let it sit for 20 minutes after washing off the sticky stuff from simmering.
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Re: Black whisker mold [Re: Lutioush]
#8328001 - 04/25/08 06:24 PM (4 months, 1 day ago) |
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there are two ways to do spawn jars, either in the jars or with the soak method. If your adding moisture to the jars after soaking and boiling then this could be the source of your problems. As for the gypsum I just add during soak and that works fine.
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"Who said every wish would be heard and answered when wished on a morning star? Some asshole thought of it, some sucker believed it and look what it's done so far." -the great LoC
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Lutioush
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I think i've figured it out. It's gotta be something in my air. I left one jar completely unshaken and about two days later had a nice layer of trich across the top. I'm going to spend the next two days or so bleaching surfaces and ousting the air in my room and glove box.
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Lutioush
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Re: Black whisker mold [Re: Lutioush]
#8331855 - 04/26/08 07:35 PM (4 months, 3 hours ago) |
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I am also going to use a liquid culture instead of a grain to grain. I made three from apple juice containers a while back and I lost 2 to cobweb and Black whisker but the third isn't showing any signs of contams after about 3 months so I'm pretty sure it's safe to use.
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