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 lipa
Registered: 07/24/07
Posts: 415
Last seen: 3 hours, 59 minutes
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 Shiitake and trametes versicolor updates.
#8259168 - 04/09/08 10:22 AM (4 months, 25 days ago) |
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I had a couple of isolated cultures selected from multispore plates that spontaneously erupted into hyphal aggregates. The plates are not very old and look very promising. These are among many I am working with right now. There are about 6 plates out of estimate of 40-50 that have shown this. Most are very uniform and are growing quite fast for shiitake but have not demonstrated this so far.
I inoculated liquid culture with these strains for inoculation into sawdust,bran,newspaper substrates.
Also used double wedge tech to inoculate 50/50 rye/wheat spawn jars.
The trametes is doing very well. Almost too well. It colonizes quart jars of rye in 4 days. It's incredible. Fastest species I have cultivated so far.
It literally tries to push itself out of the dishes and under the agar similar to what happens with oysters.
I used some spawn to inoculate 2 gallon bags of sawdust like this. I just shook it! It was almost completely white after a few days. I will post more pics after it recovers. Oh! And recovery happens over night. Yeah crazy huh! i wake up the next morning to find dime size growths.
I'm being taken over by my hobby
I need to eat!! lol
Edited by lipa (04/09/08 10:30 AM)
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vdlsar
Stranger
Registered: 11/10/07
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Last seen: 3 days, 22 hours
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Re: Shiitake and trametes versicolor updates. [Re: lipa]
#8261711 - 04/09/08 08:39 PM (4 months, 24 days ago) |
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Quote:
lipa said:
I'm being taken over by my hobby
I need to eat!! lol
"I collect spores, mold, and fungus." -- Egon Spengler, Ghostbusters. And you thought it was a joke? 
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lipa
Registered: 07/24/07
Posts: 415
Last seen: 3 hours, 59 minutes
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Re: Shiitake and trametes versicolor updates. [Re: lipa]
#8263766 - 04/10/08 09:52 AM (4 months, 24 days ago) |
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16 hours later there is thick growth. Cool Huh!
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Andrew47
Servant of allLife
Registered: 04/06/06
Posts: 390
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Re: Shiitake and trametes versicolor updates. [Re: lipa]
#8268908 - 04/11/08 11:15 AM (4 months, 23 days ago) |
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Yep, I've found T.versicolor to be a graet colonizor. The culture I'm working with now colonizes a 70mm petri from tissue scrapes in 7-8 days
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It's easy! Send your clean prints to www.fsrcanada.com
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lipa
Registered: 07/24/07
Posts: 415
Last seen: 3 hours, 59 minutes
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Re: Shiitake and trametes versicolor updates. [Re: Andrew47]
#8268941 - 04/11/08 11:24 AM (4 months, 23 days ago) |
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Could you please post a picture of it. And where it was found. I'd like to see if there is any characteristics in region. I'll trade you for one of mine. Mine has a lot of aggregates all over it after the first week. Bumpy as hell. I'll post a pic tonight of mine after work.
Here it is at 36 hours
Edited by lipa (04/11/08 11:36 AM)
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Andrew47
Servant of allLife
Registered: 04/06/06
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Re: Shiitake and trametes versicolor updates. [Re: Andrew47]
#8269021 - 04/11/08 11:41 AM (4 months, 23 days ago) |
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Hey Lipa,
I left my camera at home, but I have 10 plates colonized here in the fridge and they all are 'bumpy.' There is a ring from 35mm to 45mm (inside and outside diameter) that is distinctly 'bumpy' while the rest is flat like the Reishi. I have a G. Lucidum culture that colonizes 70mm petris in 6 days 
I'd be happy to trade. I don't think I need any cultures, but if you send me a package with the postage and your address on it I'll fill it up  PM me for the address
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It's easy! Send your clean prints to www.fsrcanada.com
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lipa
Registered: 07/24/07
Posts: 415
Last seen: 3 hours, 59 minutes
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Re: Shiitake and trametes versicolor updates. [Re: Andrew47]
#8269122 - 04/11/08 12:04 PM (4 months, 23 days ago) |
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Very similar to mine but the aggregates are all over, not on just the outer parts. How interesting!
Thanks! I'll take a pic tonight of it!
Edited by lipa (04/11/08 12:05 PM)
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Andrew47
Servant of allLife
Registered: 04/06/06
Posts: 390
Last seen: 1 day, 8 hours
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Re: Shiitake and trametes versicolor updates. [Re: lipa]
#8279345 - 04/13/08 08:08 PM (4 months, 20 days ago) |
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Quote:
lipa said:
Very similar to mine but the aggregates are all over, not on just the outer parts. How interesting!
Yep, but interestingly it *isn't* the leading edge but rather halfway between the point of inoculation and the edge
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It's easy! Send your clean prints to www.fsrcanada.com
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lipa
Registered: 07/24/07
Posts: 415
Last seen: 3 hours, 59 minutes
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Re: Shiitake and trametes versicolor updates. [Re: Andrew47]
#8280348 - 04/13/08 11:45 PM (4 months, 20 days ago) |
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Oh! I didn't know how much the culture of yours was grown out. I ussumed the 45mm was the leading edge of your culture. My bad. I don't use petris! I never pay attention to the size of the ones you guys are using nowadays. I prefere the pp deli cups. They are a little larger I think.
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 zee_werp
a fractalcreature
Registered: 03/24/03
Posts: 1,023
Loc: Aotearoa
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Re: Shiitake and trametes versicolor updates. [Re: lipa]
#8586641 - 07/02/08 02:25 AM (2 months, 3 days ago) |
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Hi, nice work. What are the plastic tubs seen in the 3rd and 4th pics down? Are they claveable?
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lipa
Registered: 07/24/07
Posts: 415
Last seen: 3 hours, 59 minutes
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Re: Shiitake and trametes versicolor updates. [Re: zee_werp]
#8587021 - 07/02/08 08:35 AM (2 months, 3 days ago) |
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Yes they are polypropylene deli cups. They are autoclavable. I love them and their cheap, and reusable.
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dstark
Yes you can but you may Not!
Registered: 02/27/08
Posts: 1,033
Loc: -CamP!ng iN mY Ro.Om-
Last seen: 15 hours, 1 minute
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Re: Shiitake and trametes versicolor updates. [Re: lipa]
#8587295 - 07/02/08 10:41 AM (2 months, 3 days ago) |
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Very nice work
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.:Darkie:.
Sucks my Sig must be only 65kb ;(
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lipa
Registered: 07/24/07
Posts: 415
Last seen: 3 hours, 59 minutes
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Re: Shiitake and trametes versicolor updates. [Re: dstark]
#8587325 - 07/02/08 10:49 AM (2 months, 3 days ago) |
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Thanks guys. Although I brought this thread up accidentally when looking for my other shiitake thread. This was the start of the experiment. When I first started having good luck with the cultures. The Trametes was placed outside and fruited, but got attacked by bugs. I will work on a second attempt.
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