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 old mushmellow
mushmaker
 
Registered: 03/15/08
Posts: 317
Loc: the dome
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 nutrient addition for incubation
#8210289 - 03/29/08 01:20 PM (8 months, 2 days ago) |
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I thought Id try an experiment to see if I could improve mycelium development. I considered the composition of psilocybin in my decision on what to try. So anyway I added a teaspoon of yeast energizer to enough standard pf substrate to make 6 half pint cakes and sterilized like usual.
Results: 1 week of growth looks like 2, an incredible difference! Has anyone else tried such a thing?
Yeast energizer contains DAP, biotin, MgSO3, and vitamin B complex. I'll update as things progress. 
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 coulterIV
Technopagan
Registered: 11/07/07
Posts: 236
Loc: marlton, new jersey
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i have been considering adding maca root to the mix.
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abbr
Stranger
Registered: 01/21/08
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 Re: nutrient addition for incubation [Re: coulterIV]
#8219465 - 03/31/08 01:35 PM (8 months, 20 hours ago) |
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I've never tried it.
But was wondering if the Yeast Energizer
is the same as Yeast Nutrient?
I mistakenly bought Fermax Yeast Nutrient when
looking for Nutritional Yeast.
I was later told by a Brew Shop it was
only nutrients for yeast.
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Yrat
Hello
Registered: 11/08/07
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Re: nutrient addition for incubation [Re: abbr]
#8219812 - 03/31/08 02:48 PM (8 months, 19 hours ago) |
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Are you running a negative control alongside your experimental jars? Next time try making six of each simultaneously so you are absolutely 100% sure of the cause for differences in growth that can't be due to some random environmental variable.
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old mushmellow
mushmaker
Registered: 03/15/08
Posts: 317
Loc: the dome
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Re: nutrient addition for incubation [Re: Yrat]
#8221293 - 03/31/08 07:34 PM (8 months, 14 hours ago) |
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yeast energizer has a little more in it than yeast nutrient. I will do a control batch next time, sorry I didn't this time . I will do it for science sake. a very good point. I am going by experience.
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Knifeplay
HAIR POLICE
Registered: 03/21/08
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Re: nutrient addition for incubation [Re: old mushmellow]
#8221681 - 03/31/08 08:54 PM (8 months, 12 hours ago) |
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This seems like it could go places. I've read good things about spirulina.
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old mushmellow
mushmaker
Registered: 03/15/08
Posts: 317
Loc: the dome
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Re: nutrient addition for incubation [Re: Knifeplay]
#8223198 - 04/01/08 06:38 AM (8 months, 3 hours ago) |
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Actually I was hoping to improve potency. My initial results were a surprise.
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shoeareyou
Stranger
Registered: 01/07/07
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Quote:
old mushmellow said:
I thought Id try an experiment to see if I could improve mycelium development. I considered the composition of psilocybin in my decision on what to try. So anyway I added a teaspoon of yeast energizer to enough standard pf substrate to make 6 half pint cakes and sterilized like usual.
Results: 1 week of growth looks like 2, an incredible difference! Has anyone else tried such a thing?
Yeast energizer contains DAP, biotin, MgSO3, and vitamin B complex. I'll update as things progress.
Ive never tried Yeast energiser, but i am very fond of adding yeast extract (marmite) to my experiments. If you consider that yeast itself is a fungus, the cells must have every component necissary for formation of more fungal cells. I like the idea of the accumulated micronutrients too, going directly into the mycellium. I have a sneaky suspicion that the media we are using BRF/Straw/Rye Grain are too pure and refined for optimal growth. what about all those essential B vitamins, amino acids etc? surely they can't all be present in one foodstuff.
This especially goes for karo LC's btw, I am almost 100% Sure that karo doesn't have everything necissary for good mycellial growth, but it seems to work despite this...
Will get back to you with the results of my experiment in 2 weeks time.
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old mushmellow
mushmaker
Registered: 03/15/08
Posts: 317
Loc: the dome
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Re: nutrient addition for incubation [Re: shoeareyou]
#8223762 - 04/01/08 10:30 AM (7 months, 30 days ago) |
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I hadn't considered an amino acid supplement. any suggestions?
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creekfreek
Just being me
Registered: 01/26/08
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Re: nutrient addition for incubation [Re: old mushmellow]
#8223833 - 04/01/08 11:07 AM (7 months, 30 days ago) |
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If I can find the yeast energizer, I will try it out. My jars are almost 100% now so as soon as I get the cakes out of them I will do half with the yeast energizer and half just regular PF jars.
I will add my results here also.
Peace.
And thanks for the idea.
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"Recite it all back to me at the end of the story and we'll see if we know what we mean" - Scott Murawski.
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coulterIV
Technopagan
Registered: 11/07/07
Posts: 236
Loc: marlton, new jersey
Last seen: 5 days, 7 hours
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Re: nutrient addition for incubation [Re: old mushmellow]
#8224017 - 04/01/08 12:00 PM (7 months, 30 days ago) |
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Quote:
old mushmellow said:
I hadn't considered an amino acid supplement. any suggestions?:)
thats why i suggested maca. it has vitamins minerals and a complete amino acid profile. its a powdered root from the andes mountains. i will try it out soon
Nutritional Profile of Dried Maca Root
(Average 10 gram serving)
Component per 10 g Amino Acids per 10 g Minerals per 10 g
Protein 1–1.4 g Alanine 63.1 mg Calcium 25 mg
Carbohydrates 6–7.5 g Arginine 99.4 mg Copper 0.6 mg
Fats (lipids) 220 mg Aspartic acid 91.7 mg Iron 1.5 mg
Fiber 850 mg Glutamic acid 156.5 mg Iodine 52 mcg
Ash 490 mg Glycine 68.3 mg Manganese 80 mcg
Sterols 5–10 mg Histidine 41.9 mg Potassium 205 mg
Calories 32.5 HO-Proline 26.0 mg Sodium 1.9 mg
Isoleucine 47.4 mg Zinc 380 mcg
Leucine 91.0 mg
Vitamins per 10 g Lysine 54.5 mg Fats/Lipids per 10 g
B2 39 mcg Methionine 28.0 mg Linoleic 72 mcg
B6 114 mcg Phenylalanine 55.3 mg Palmitic 52 mcg
C 28.6 mg Proline 0.5 mg Oleic 24.5 mcg
Niacin 565 mcg Sarcosine 0.7 mg
Serine 50.4 mg
Threonine 33.1 mg
Tryptophan 4.9 mg
Tyrosine 30.6 mg
Valine 79.3 mg
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old mushmellow
mushmaker
Registered: 03/15/08
Posts: 317
Loc: the dome
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Re: nutrient addition for incubation [Re: coulterIV]
#8224112 - 04/01/08 12:24 PM (7 months, 30 days ago) |
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Where do you get maca root powder? A health food store?
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coulterIV
Technopagan
Registered: 11/07/07
Posts: 236
Loc: marlton, new jersey
Last seen: 5 days, 7 hours
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Re: nutrient addition for incubation [Re: old mushmellow]
#8229448 - 04/02/08 04:22 PM (7 months, 29 days ago) |
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Quote:
old mushmellow said:
Where do you get maca root powder? A health food store?
whole foods, pm me if you want some though.
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BREATHE IN LOVE
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old mushmellow
mushmaker
Registered: 03/15/08
Posts: 317
Loc: the dome
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Re: nutrient addition for incubation [Re: old mushmellow]
#8259058 - 04/09/08 09:36 AM (7 months, 23 days ago) |
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birthed at day 14. looking good so far. Fast results. no serious contam probs but I did lose two jars to green pen..
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danknugz81
spiralingdownward
Registered: 02/22/08
Posts: 773
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Re: nutrient addition for incubation [Re: old mushmellow]
#8260946 - 04/09/08 06:19 PM (7 months, 22 days ago) |
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would something like this work for rye grain as well or just brf substrate?
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old mushmellow
mushmaker
Registered: 03/15/08
Posts: 317
Loc: the dome
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Re: nutrient addition for incubation [Re: danknugz81]
#8268282 - 04/11/08 05:47 AM (7 months, 21 days ago) |
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I think it can be used with any substrate even agar.
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old mushmellow
mushmaker
Registered: 03/15/08
Posts: 317
Loc: the dome
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Re: nutrient addition for incubation [Re: old mushmellow]
#8357199 - 05/03/08 10:35 AM (6 months, 29 days ago) |
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Well, I've had two flushes so far with extremely healthy and vigorous mushrooms. No cake contamination once in the terrarium. I did notice an improved potency and I actually tripped mildly on one dry gram. I thought that was pretty good.
My result: Yeast energizer actually was beneficial both in strength, vigor and potency.
I invite anyone with good experience to try my experiment again with a negative control and give your results. 
Here's a pic:
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 x7x_x7x
urban farmer
Registered: 05/05/07
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Re: nutrient addition for incubation [Re: old mushmellow]
#8360857 - 05/04/08 10:48 AM (6 months, 28 days ago) |
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plain, commercial grade yeast, the used in foods, is a component o f Sabouraud culture medium
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amanitavirosa
absurdlyoptimistic
Registered: 04/07/08
Posts: 336
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Re: nutrient addition for incubation [Re: x7x_x7x]
#8360909 - 05/04/08 11:05 AM (6 months, 28 days ago) |
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I wouldn't waste my money on health food store additives - sure you might see some effect (growth rate, and maybe increased potency) but is it actually worth blowing lots of $$ on?
Go with yeast extract if anything since its cheap - but I would shy away from any other additives such as amino acid powders, etc.
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x7x_x7x
urban farmer
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Re: nutrient addition for incubation [Re: amanitavirosa]
#8361005 - 05/04/08 11:35 AM (6 months, 28 days ago) |
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nobody can warrant an increase in potency; yeast add nutrient content.
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FooMan
Registered: 02/02/05
Posts: 6,262
Loc: Amsterdam
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Re: nutrient addition for incubation [Re: old mushmellow]
#8361423 - 05/04/08 01:57 PM (6 months, 28 days ago) |
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Coffee tends to speed mycelium growth. I've never experimented with yeast. As far as potency goes, it's in the genetics.
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psilociety
free to be
Registered: 02/01/08
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Re: nutrient addition for incubation [Re: FooMan]
#8382610 - 05/09/08 05:57 PM (6 months, 23 days ago) |
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i use chaft(spelling?), which is the soft shell of green coffee beans. i work at a roastery so i can take as much of it as i want. it is very fluffy and absorbent.
i would love to try maca in a substrate. i brought back a few kilos from peru. it is what the inkan warriors ate before battle. they werent supposed to eat it after battle because it is a sexual potentiator and they are more likely to rape their victims.
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quickpick
Stranger
Registered: 08/15/08
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Re: nutrient addition for incubation [Re: old mushmellow]
#8785121 - 08/17/08 01:52 AM (3 months, 17 days ago) |
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Hey old mushmellow,
That's a real good idea and I have some right here. I'm gonna add some to my bulk sub and casing and maybe rye jars too.
I do a lot of fermenting of microbes (EM, BIM, IMO, etc) and I use "Pure Pitchable Yeast" which is live yeast cells. Anyway, inside the package is a "food package" for the yeast (brewers "ale" yeast);this may or may not be the same as "yeast nutrient". I will look into the contents and that maybe an option too. It might be good to try the food packs or at least see what it's made of (simple sugar I assume)
Also of note is the organic substance "inulin". It's most prevent in roots of some plants and grasses and it's a base carbon source. It's my position that AM (arbuscular mycrrihzeas) consume inulin once they penetrate the roots of the host in trade for giving the roots water, protection and solubleize P. I have desinged a DIY method to extact the inulin from roots with using heat and pressure in the kitchen. I can post more if your interested. Many microbes will 'eat' inulin...
Edited by quickpick (08/17/08 02:00 AM)
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old mushmellow
mushmaker
Registered: 03/15/08
Posts: 317
Loc: the dome
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Re: nutrient addition for incubation [Re: quickpick]
#8843101 - 08/28/08 05:41 PM (3 months, 5 days ago) |
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Thanks! It seems to help, especially with ZZZZ strain. wooohooo!
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Steve
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Re: nutrient addition for incubation [Re: x7x_x7x]
#8846096 - 08/29/08 07:03 AM (3 months, 5 days ago) |
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Sorry to bring up an old topic but i had a question.
Shoeareyou - Do you just dissolve the Marmite into the water before adding it to the substrate?? Im not experienced but this would seem logical to me....
How did the tests go??
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