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 old mushmellow
mushmaker
 
Registered: 03/15/08
Posts: 253
Loc: the dome
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 nutrient addition for incubation
#8210289 - 03/29/08 01:20 PM (4 months, 28 days ago) |
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I thought Id try an experiment to see if I could improve mycelium development. I considered the composition of psilocybin in my decision on what to try. So anyway I added a teaspoon of yeast energizer to enough standard pf substrate to make 6 half pint cakes and sterilized like usual.
Results: 1 week of growth looks like 2, an incredible difference! Has anyone else tried such a thing?
Yeast energizer contains DAP, biotin, MgSO3, and vitamin B complex. I'll update as things progress. 
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It is tempting to say that one gets from the LSD encounter what one deserves, "Quidquid recipietur secundum modum recipient is recipietur" our nature determines what we receive.
Dr. sidney Cohen
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 coulterIV
Technopagan
Registered: 11/07/07
Posts: 227
Loc: marlton, new jersey
Last seen: 13 days, 3 hours
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 Re: nutrient addition for incubation [Re: old mushmellow]
#8210753 - 03/29/08 03:18 PM (4 months, 28 days ago) |
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i have been considering adding maca root to the mix.
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BREATHE IN LOVE
BREATHE OUT FORGIVENESS
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abbr
Stranger
Registered: 01/21/08
Posts: 1
Last seen: 4 months, 15 days
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Re: nutrient addition for incubation [Re: coulterIV]
#8219465 - 03/31/08 01:35 PM (4 months, 26 days ago) |
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I've never tried it.
But was wondering if the Yeast Energizer
is the same as Yeast Nutrient?
I mistakenly bought Fermax Yeast Nutrient when
looking for Nutritional Yeast.
I was later told by a Brew Shop it was
only nutrients for yeast.
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Yrat
Hello
Registered: 11/08/07
Posts: 221
Last seen: 5 hours, 24 minutes
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Re: nutrient addition for incubation [Re: abbr]
#8219812 - 03/31/08 02:48 PM (4 months, 26 days ago) |
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Are you running a negative control alongside your experimental jars? Next time try making six of each simultaneously so you are absolutely 100% sure of the cause for differences in growth that can't be due to some random environmental variable.
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old mushmellow
mushmaker
Registered: 03/15/08
Posts: 253
Loc: the dome
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Re: nutrient addition for incubation [Re: Yrat]
#8221293 - 03/31/08 07:34 PM (4 months, 26 days ago) |
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yeast energizer has a little more in it than yeast nutrient. I will do a control batch next time, sorry I didn't this time . I will do it for science sake. a very good point. I am going by experience.
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It is tempting to say that one gets from the LSD encounter what one deserves, "Quidquid recipietur secundum modum recipient is recipietur" our nature determines what we receive.
Dr. sidney Cohen
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Knifeplay
HAIR POLICE
Registered: 03/21/08
Posts: 163
Last seen: 22 days, 17 hours
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Re: nutrient addition for incubation [Re: old mushmellow]
#8221681 - 03/31/08 08:54 PM (4 months, 26 days ago) |
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This seems like it could go places. I've read good things about spirulina.
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bmiles: shrooms are 90% cum.
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old mushmellow
mushmaker
Registered: 03/15/08
Posts: 253
Loc: the dome
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Re: nutrient addition for incubation [Re: Knifeplay]
#8223198 - 04/01/08 06:38 AM (4 months, 25 days ago) |
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Actually I was hoping to improve potency. My initial results were a surprise.
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It is tempting to say that one gets from the LSD encounter what one deserves, "Quidquid recipietur secundum modum recipient is recipietur" our nature determines what we receive.
Dr. sidney Cohen
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shoeareyou
Stranger
Registered: 01/07/07
Posts: 195
Last seen: 3 months, 12 days
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Re: nutrient addition for incubation [Re: old mushmellow]
#8223380 - 04/01/08 08:19 AM (4 months, 25 days ago) |
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Quote:
old mushmellow said:
I thought Id try an experiment to see if I could improve mycelium development. I considered the composition of psilocybin in my decision on what to try. So anyway I added a teaspoon of yeast energizer to enough standard pf substrate to make 6 half pint cakes and sterilized like usual.
Results: 1 week of growth looks like 2, an incredible difference! Has anyone else tried such a thing?
Yeast energizer contains DAP, biotin, MgSO3, and vitamin B complex. I'll update as things progress.
Ive never tried Yeast energiser, but i am very fond of adding yeast extract (marmite) to my experiments. If you consider that yeast itself is a fungus, the cells must have every component necissary for formation of more fungal cells. I like the idea of the accumulated micronutrients too, going directly into the mycellium. I have a sneaky suspicion that the media we are using BRF/Straw/Rye Grain are too pure and refined for optimal growth. what about all those essential B vitamins, amino acids etc? surely they can't all be present in one foodstuff.
This especially goes for karo LC's btw, I am almost 100% Sure that karo doesn't have everything necissary for good mycellial growth, but it seems to work despite this...
Will get back to you with the results of my experiment in 2 weeks time.
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p. cubensis blue meanies/cambodian
p. azurescens & p.semilancaeta:
http://www.shroomery.org/forums/showflat.php/Number/8191028#8191028
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old mushmellow
mushmaker
Registered: 03/15/08
Posts: 253
Loc: the dome
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Re: nutrient addition for incubation [Re: shoeareyou]
#8223762 - 04/01/08 10:30 AM (4 months, 25 days ago) |
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I hadn't considered an amino acid supplement. any suggestions?
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It is tempting to say that one gets from the LSD encounter what one deserves, "Quidquid recipietur secundum modum recipient is recipietur" our nature determines what we receive.
Dr. sidney Cohen
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creekfreek
Just being me
Registered: 01/26/08
Posts: 1,977
Loc: Right about here
Last seen: 1 hour, 41 minutes
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Re: nutrient addition for incubation [Re: old mushmellow]
#8223833 - 04/01/08 11:07 AM (4 months, 25 days ago) |
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If I can find the yeast energizer, I will try it out. My jars are almost 100% now so as soon as I get the cakes out of them I will do half with the yeast energizer and half just regular PF jars.
I will add my results here also.
Peace.
And thanks for the idea.
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"Recite it all back to me at the end of the story and we'll see if we know what we mean" - Scott Murawski.
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coulterIV
Technopagan
Registered: 11/07/07
Posts: 227
Loc: marlton, new jersey
Last seen: 13 days, 3 hours
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Re: nutrient addition for incubation [Re: old mushmellow]
#8224017 - 04/01/08 12:00 PM (4 months, 25 days ago) |
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Quote:
old mushmellow said:
I hadn't considered an amino acid supplement. any suggestions?:)
thats why i suggested maca. it has vitamins minerals and a complete amino acid profile. its a powdered root from the andes mountains. i will try it out soon
Nutritional Profile of Dried Maca Root
(Average 10 gram serving)
Component per 10 g Amino Acids per 10 g Minerals per 10 g
Protein 1–1.4 g Alanine 63.1 mg Calcium 25 mg
Carbohydrates 6–7.5 g Arginine 99.4 mg Copper 0.6 mg
Fats (lipids) 220 mg Aspartic acid 91.7 mg Iron 1.5 mg
Fiber 850 mg Glutamic acid 156.5 mg Iodine 52 mcg
Ash 490 mg Glycine 68.3 mg Manganese 80 mcg
Sterols 5–10 mg Histidine 41.9 mg Potassium 205 mg
Calories 32.5 HO-Proline 26.0 mg Sodium 1.9 mg
Isoleucine 47.4 mg Zinc 380 mcg
Leucine 91.0 mg
Vitamins per 10 g Lysine 54.5 mg Fats/Lipids per 10 g
B2 39 mcg Methionine 28.0 mg Linoleic 72 mcg
B6 114 mcg Phenylalanine 55.3 mg Palmitic 52 mcg
C 28.6 mg Proline 0.5 mg Oleic 24.5 mcg
Niacin 565 mcg Sarcosine 0.7 mg
Serine 50.4 mg
Threonine 33.1 mg
Tryptophan 4.9 mg
Tyrosine 30.6 mg
Valine 79.3 mg
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BREATHE IN LOVE
BREATHE OUT FORGIVENESS
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old mushmellow
mushmaker
Registered: 03/15/08
Posts: 253
Loc: the dome
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Re: nutrient addition for incubation [Re: coulterIV]
#8224112 - 04/01/08 12:24 PM (4 months, 25 days ago) |
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Where do you get maca root powder? A health food store?
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It is tempting to say that one gets from the LSD encounter what one deserves, "Quidquid recipietur secundum modum recipient is recipietur" our nature determines what we receive.
Dr. sidney Cohen
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coulterIV
Technopagan
Registered: 11/07/07
Posts: 227
Loc: marlton, new jersey
Last seen: 13 days, 3 hours
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Re: nutrient addition for incubation [Re: old mushmellow]
#8229448 - 04/02/08 04:22 PM (4 months, 24 days ago) |
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Quote:
old mushmellow said:
Where do you get maca root powder? A health food store?
whole foods, pm me if you want some though.
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BREATHE IN LOVE
BREATHE OUT FORGIVENESS
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old mushmellow
mushmaker
Registered: 03/15/08
Posts: 253
Loc: the dome
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Re: nutrient addition for incubation [Re: old mushmellow]
#8259058 - 04/09/08 09:36 AM (4 months, 17 days ago) |
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birthed at day 14. looking good so far. Fast results. no serious contam probs but I did lose two jars to green pen..
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It is tempting to say that one gets from the LSD encounter what one deserves, "Quidquid recipietur secundum modum recipient is recipietur" our nature determines what we receive.
Dr. sidney Cohen
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danknugz81
spiralingdownward
Registered: 02/22/08
Posts: 769
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Re: nutrient addition for incubation [Re: old mushmellow]
#8260946 - 04/09/08 06:19 PM (4 months, 17 days ago) |
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would something like this work for rye grain as well or just brf substrate?
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old mushmellow
mushmaker
Registered: 03/15/08
Posts: 253
Loc: the dome
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Re: nutrient addition for incubation [Re: danknugz81]
#8268282 - 04/11/08 05:47 AM (4 months, 15 days ago) |
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I think it can be used with any substrate even agar.
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It is tempting to say that one gets from the LSD encounter what one deserves, "Quidquid recipietur secundum modum recipient is recipietur" our nature determines what we receive.
Dr. sidney Cohen
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old mushmellow
mushmaker
Registered: 03/15/08
Posts: 253
Loc: the dome
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Re: nutrient addition for incubation [Re: old mushmellow]
#8357199 - 05/03/08 10:35 AM (3 months, 24 days ago) |
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Well, I've had two flushes so far with extremely healthy and vigorous mushrooms. No cake contamination once in the terrarium. I did notice an improved potency and I actually tripped mildly on one dry gram. I thought that was pretty good.
My result: Yeast energizer actually was beneficial both in strength, vigor and potency.
I invite anyone with good experience to try my experiment again with a negative control and give your results. 
Here's a pic:
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It is tempting to say that one gets from the LSD encounter what one deserves, "Quidquid recipietur secundum modum recipient is recipietur" our nature determines what we receive.
Dr. sidney Cohen
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x7x_x7x
another one
Registered: 05/05/07
Posts: 610
Last seen: 1 hour, 37 minutes
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Re: nutrient addition for incubation [Re: old mushmellow]
#8360857 - 05/04/08 10:48 AM (3 months, 23 days ago) |
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plain, commercial grade yeast, the used in foods, is a component o f Sabouraud culture medium
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transhumanism + primitivism=magic mushrooms
SuctoSpore®
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amanitavirosa
absurdlyoptimistic
Registered: 04/07/08
Posts: 331
Loc: Cold places
Last seen: 7 hours, 43 minutes
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Re: nutrient addition for incubation [Re: x7x_x7x]
#8360909 - 05/04/08 11:05 AM (3 months, 23 days ago) |
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I wouldn't waste my money on health food store additives - sure you might see some effect (growth rate, and maybe increased potency) but is it actually worth blowing lots of $$ on?
Go with yeast extract if anything since its cheap - but I would shy away from any other additives such as amino acid powders, etc.
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x7x_x7x
another one
Registered: 05/05/07
Posts: 610
Last seen: 1 hour, 37 minutes
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Re: nutrient addition for incubation [Re: amanitavirosa]
#8361005 - 05/04/08 11:35 AM (3 months, 23 days ago) |
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nobody can warrant an increase in potency; yeast add nutrient content.
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transhumanism + primitivism=magic mushrooms
SuctoSpore®
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