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 tomaquinas539
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Registered: 10/10/07
Posts: 56
Last seen: 3 years, 9 months
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 my rhizomorphic myc - question on isolated strains 
#7988662 - 02/06/08 09:46 PM (5 years, 3 months ago) |
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I was just noobing around to find some less variable controls for my conditions. Here is the deal: 1/4 pint jar; 4 small nailed holes covered with 2 layers or real medical micropore tape; poured agar from a tall jar (agar: common malt agar mixed myself); inoculated with 1/2 cc maztapec spores (from vendor).
Two weeks small cotton-like myc. Now almost another two weeks later I see about 140 degrees of obvious rhizomorphic growth expanded off that cotton button. The growth is slow (could be due to baddly measured agar mix or whatever) but 10 sections could easily be taken to new agar.
I don't understand the isolation thing biologically. So the cottony part is the hyphae of di(don't remember term) that was created by two spore's hyphae coming together. Then they create the rhyzomorphic growth. Is that cottony button in the middle just that di(? please give term). And if that rhyzomorphic growth that is somewhat slow always going to be slow colonizer if cloned from this tissue assuming the genetics of this is the lowest common denominator?
I am going to isolate it later anyway, but another question is, is that all di(?) or when is isolate am I taking small pieces so the strongest di() will flourish over other?
I am some what confused by this, I want to isolate this but I could just as easily place a colonized rye grain that has some aggresive myc on it then isolate that instead. I don't have any petri dishes at the moment so for the next month or so i am bound by 12 1/4 pint jars.
Just for more info on my conditions (if it helps). I did this to 3 jars. one contamed with the mean green. and the other of the three had myc and I transfered to quart rye, that is getting some really slow growth. Say about maybe 5% in a week since transfer.
Thanks for everything people 
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 tahoe
Noob Slayer
Registered: 11/27/03
Posts: 5,420
Loc: N38.93829W119.98108
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Re: my rhizomorphic myc - question on isolated strains [Re: tomaquinas539]
#7988979 - 02/06/08 10:31 PM (5 years, 3 months ago) |
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you can get rhizomorphic growth out of the cottony sectors. You want to start worrying about rhizos after you transfer it away from the spore plate.
Pick a nice section and put it to another plate. Watch it, and than transfer the fastest/best looking section from that one.
This was transfered from the pertri that had spores on it.
it is showing at least two sectors maybe 3,4
It was actually transfered from this one.
notice all the differnt types of growth.
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The subjects that I cover are not species specific and can be used in All Aspects of Mycology
I really need a good flow hood and a scope
Teh=The
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