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 lorbitherize
Registered: 11/24/05
Posts: 247
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 Concerning Blenders & Tissue Culturing
#7875426 - 01/13/08 08:12 PM (7 months, 18 days ago) |
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I've done a bit of searching through posts on
this, and have found that 'Oster' seems to be
well-renound for their blender design as it
benefits the production of liquid inoculant(s).
The ultimate goal is, of course to assemble the
parts necessary to aseptically 'blend' mycellial
tissue in pursuit of obtaining mass quantities
of said liquid inoculant, which simple as it may
sound requires parts of specific physical dimen-
sions.
Secondary to the ultimate goal would naturally
be to avoid spending prohibitive amounts of cash.
This raises the question of which model of
blender one is to choose from amongst the whole
confusing gigantic selection of Oster-made all-
metal-blade-assembly blenders...
?
...due to the necessity of finding one capable
of integrating with the standard size of Ball
and Kerr mason jars, naturally.
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lorbitherize
Registered: 11/24/05
Posts: 247
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 Re: Concerning Blenders & Tissue Culturing [Re: lorbitherize]
#7879687 - 01/14/08 06:31 PM (7 months, 17 days ago) |
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Well, after looking at replacement Osterizer blender parts
I guess I've answered my own question... all of their blade
assemblies are the same standard size.
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 ranonar
Stranger
Registered: 07/27/06
Posts: 26
Last seen: 1 month, 6 days
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Re: Concerning Blenders & Tissue Culturing [Re: lorbitherize]
#7887417 - 01/16/08 07:04 AM (7 months, 15 days ago) |
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If you really want to avoid making costs then don't buy a blender. You really don't need one. A good low-cost alternative is to make agar cultures in whiskey bottles (it works also in other bottles but the flat sides of whiskey bottles provide a large surface while you do not need much agar). When the agar surface is colonized you can fill the bottle for 1/4 with water, shake like hell for a minute, fill the bottle completely and you are ready to inoculate.
I love this method because you do not need to take the culture out to make a slurry and of course because you can do without a blender.
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d4a2n0k
The Dude
Registered: 07/23/03
Posts: 521
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Re: Concerning Blenders & Tissue Culturing [Re: lorbitherize]
#7887811 - 01/16/08 10:40 AM (7 months, 15 days ago) |
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If cost is an issue, look at thrift stores/second hand stores. I got a perfect blender for this purpose for $2.
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Row, row, row your boat,
Gently down the stream.
Merrily, merrily, merrily, merrily,
Life is but a dream.
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lorbitherize
Registered: 11/24/05
Posts: 247
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Re: Concerning Blenders & Tissue Culturing [Re: ranonar]
#7893959 - 01/17/08 05:01 PM (7 months, 14 days ago) |
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 blenderTek_TVGuide.pdf (3,359,995 bytes)
38 downloads [Copyrighted?]
The thing that spurred me to go the
'blender route' was a thread over at
Mycotopia... it looks really awesome!
I've attached it to this message in
.pdf form - hopefully thats alright
with its author...
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The shroomy 1
Let me be YOUR"guide"
Registered: 03/27/07
Posts: 1,868
Loc: Texas
Last seen: 4 hours, 34 minutes
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Re: Concerning Blenders & Tissue Culturing [Re: lorbitherize]
#7894334 - 01/17/08 06:40 PM (7 months, 14 days ago) |
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Why don't you make a magnetic stirrer? Very simple and relatively inexpensive to make.
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fastfred
Registered: 05/17/04
Posts: 4,326
Loc: Dark side of the moon
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Re: Concerning Blenders & Tissue Culturing [Re: The shroomy 1]
#7898357 - 01/18/08 05:27 PM (7 months, 13 days ago) |
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> Why don't you make a magnetic stirrer?
Mag stirrers don't exactly blend now do they?
I've thought of trying to add a razor blade to a stir bar, but I have never tried it.
-FF
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ranonar
Stranger
Registered: 07/27/06
Posts: 26
Last seen: 1 month, 6 days
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Re: Concerning Blenders & Tissue Culturing [Re: lorbitherize]
#7900496 - 01/19/08 04:44 AM (7 months, 12 days ago) |
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Ok I have read the attached blender tek. Here is a simplefied version. Cut a wedge from a colonized cake (use a flame-sterilized scalpel) and put it in a clean tall jar (or bottle). I commonly use 100ml shoulderless jars for that purpose but shoulderlessness is not a main concern here. In the jar is a concentration of 0.15 percent hydrogen peroxide (i.e. one part standard 3 percent hydrogen peroxide diluted with 19 volume parts of tapwater) and the jar is for about 20 percent full. Throw the wedge in, close the jar and shake like hell for a minute, then fill the jar completely with tapwater. Wait five minutes (this allows the peroxide to kill unicellular contaminants), fill your syringe(s) and inject new substrate.
Be aware that this mixture kills the mycelium over time, especially the part which is submerged, best is to use the syringes within 24 hours.
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petridish
gutted mycophile
Registered: 02/23/07
Posts: 19
Last seen: 6 months, 24 days
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Re: Concerning Blenders & Tissue Culturing [Re: lorbitherize]
#7923789 - 01/24/08 11:04 AM (7 months, 7 days ago) |
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A small mouth mason jar fits inside most standard $20 blender bases. Fill 1/3 to 2/3 full of distilled water and screw on the seal, base, and ring. Just remember not to completely tighten the lid when autoclaving. It will melt down if steam cannot escape, been there done that. Place the cooker/autoclave in front of your hood to cool and tighten jar when you open the autoclave. That's how the sterile technique works with household goods. I enjoy using this method with spores, clones, and cakes. You could even replace the water with a liquid culture and drop a chunk of shroom in for shredding and cloning. I feel like I get a lot of bang for my buck using my hillbilly eberbach. As a word of caution, if you contaminate any part of this, you will pass it to everything you inoculate. I've not had consistent success with peroxide, so I don't fool with it.
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