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 Captain Cubensis
Bleeding HeartLiberal
Registered: 09/18/07
Posts: 648
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 Strain isolation from a single colonized rye grain? 
#7843805 - 01/07/08 04:32 PM (5 years, 4 months ago) |
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Would a single grain of colonized rye, used for a G2G constitute a strain isolation?
Could there be more than one strain on a single berry?
Some people claim that at the time of 100% colonization the substrate brick is one homogeneous genetic entity, is this true?
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 Fraggin
Multi-Faceted
Registered: 01/05/05
Posts: 8,707
Last seen: 1 year, 8 months
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 Re: Isolation from a single colonized rye grain? [Re: Captain Cubensis]
#7843813 - 01/07/08 04:34 PM (5 years, 4 months ago) |
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I had a similar question between my ears today. (I was thinking a single colonized wbs grain to agar) I hope to see some good answers.
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Captain Cubensis
Bleeding HeartLiberal
Registered: 09/18/07
Posts: 648
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Re: Isolation from a single colonized rye grain? [Re: Fraggin]
#7843838 - 01/07/08 04:42 PM (5 years, 4 months ago) |
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By the time a jar colonizes, it is usually one strain, down from hundreds at germination.
It's best to fruit them separately, but if you're going to use three multispore jars in the same substrate, then adding one more won't matter one whit.
It doesn't matter the name on the syringe. What you actually have is four 'strains' at this point. Mix them all you want. The one jar that had a different name on the syringe is little different from the other three. Look at strains similar to 'races' in humans or 'breeds' in dogs.
A German Shepard can easily mate with a rottweiler, and a human from Siberia can easily mate with a human from south America.
RR
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 39,269
Loc: USA Mountain Northwest
Last seen: 5 days, 22 hours
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Re: Isolation from a single colonized rye grain? [Re: Captain Cubensis]
#7843856 - 01/07/08 04:46 PM (5 years, 4 months ago) |
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However, not all strains are compatible, so there's always the chance that a grain of rye will have more than one strain, but not likely. You'll want to start with spores on agar in order to isolate lots of strains. By isolating many strains, you'll be able to fruit each one individually, thus finding the stellar performer(s).
RR
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Captain Cubensis
Bleeding HeartLiberal
Registered: 09/18/07
Posts: 648
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Re: Isolation from a single colonized rye grain? [Re: Captain Cubensis]
#7843857 - 01/07/08 04:46 PM (5 years, 4 months ago) |
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So could you mate strains by adding 50/50 spawn to the bulk sub?
Sounds like you could to me, maybe the chance of hybrid is minuscule.
If you continuously, over time, years maybe, always mixed your fastest B+ with your fastest Penis Envy, 50/50, wouldn't eventually you get a fruit that was a beautiful hybrid of the two, from which you could clone a line.
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