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 lorbitherize
mush gushing
Registered: 11/24/05
Posts: 320
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 Culturing /w Pectin
#7738926 - 12/10/07 12:35 AM (11 months, 20 days ago) |
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In a short while I will be in a favorable position
to be working with culture plates, as recently I have
been developing my very first glovebox system - nearly
complete, and with anticipation I was shopping at
the local grocery store in their jam/jelly isle while
noticing that the pre-packaged boxes of pectin they
carry are laced with ascorbic acid. Not really wanting
to spend actual money for what may turn out in the end
not to even work, I thought I'd contribute my findings
and ask if anyone might be familiar with using this
same type of store-bought fruit pectin in their own
culturing endeavors... {?}
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 FreeSporePrints
stronzo chilegge
Registered: 03/06/05
Posts: 910
Loc: Rome, ITALY
Last seen: 2 days, 4 hours
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hello lorbitherize,
unlucky don't have the thread appeared on FreeSporePrints.org, but a Lithuan or Australian user said that pectin can't be used for culturing, because the enzymes of the mycelium break it and into the plate you'll have not a solid culture but a slimish culture 
The mycelium is really a buctcher 
Fabio
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 18,223
Loc: USA Mountain Northwest
Last seen: 9 hours, 55 minutes
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Correct. It will turn to slush. Search jello on this board. You want to use agar.
RR
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www.mushroomvideos.com
"I wouldn't want to belong to any club that would have me as a member".
Mark Twain, Woody Allen, Groucho Marx, and anyone else who wishes to claim credit for the quote.
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lorbitherize
mush gushing
Registered: 11/24/05
Posts: 320
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 Re: Culturing /w Pectin [Re: RogerRabbit]
#7741082 - 12/10/07 02:58 PM (11 months, 19 days ago) |
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Slush... darn. Well, I may have to use agar then
after all, but that doesn't mean I have to like it.
What would happen if the world's supply of agar
somehow diminished to the point where it became
prohibitively expensive? What would we all use
then? Would fungal tissue culturing become a
thing of the past?
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 18,223
Loc: USA Mountain Northwest
Last seen: 9 hours, 55 minutes
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If something happens that kills off all the seaweed in the oceans, we'll have far greater worries than the price of agar.
RR
--------------------
www.mushroomvideos.com
"I wouldn't want to belong to any club that would have me as a member".
Mark Twain, Woody Allen, Groucho Marx, and anyone else who wishes to claim credit for the quote.
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lorbitherize
mush gushing
Registered: 11/24/05
Posts: 320
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Re: Culturing /w Pectin [Re: RogerRabbit]
#7743310 - 12/10/07 11:31 PM (11 months, 19 days ago) |
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What about cooked lasagna noodles? By taking
a petri dish, inverting it vertically so as to
align the rim flush with the surface of a
noodle and applying pressure, it would be left
with a circular noodle-cookie on the inside.
Alas, what with the extinction of agar-producing
lifeforms, it would also be an unlikely cooincidence
to find semolina-containing products as well, I
must concede.
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BlimeyGrimey
Pyon!
Registered: 08/24/05
Posts: 1,189
Loc: Seattle, Wa
Last seen: 2 hours, 43 minutes
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Confirmed:
Psilocybe stuntzii
Psilocybe cyanescens
Psilocybe guilartensis
Panaeolus olivaceus
Unknown:
Washington Psilocybe species
Washington Panaeolus species
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lorbitherize
mush gushing
Registered: 11/24/05
Posts: 320
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somebody041
bud tester
Registered: 06/04/07
Posts: 449
Loc: California
Last seen: 8 hours, 13 minutes
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Why don't you just buy some agar? It's not that expensive and alot easier than trying to fit a noodle in a petri dish.
edit: and it works
Edited by somebody041 (12/12/07 08:37 PM)
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lorbitherize
mush gushing
Registered: 11/24/05
Posts: 320
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Re: Culturing /w Pectin [Re: somebody041]
#7754193 - 12/13/07 03:14 PM (11 months, 16 days ago) |
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Quote:
somebody041 said:
Why don't you just buy some agar? It's not that expensive and alot easier than trying to fit a noodle in a petri dish.
edit: and it works
I probably will... and I will also probably experiment
with some other ways of doing it without agar.
I don't much care what it costs, I'm just trying to
develop an alternate way of culturing.
Edit: It doesn't make sense to me that there is no
other method of culturing that would work just as
well as using agar.
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Edited by lorbitherize (12/13/07 03:18 PM)
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