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So, there has been a little trouble with an agar/ liquid culture solution. First a medium was prepared of agar with one of the teks, something like 1 part agar to two parts malt extract. How ever do to some mystery reason(perhaps not enough agar was used in combo with the water) it never solidified into a gel, just a thick goop. On a side note some of the sugars had seemed to have colonized... Playing renegade, some spores were added to the gel(alot of spores, they were knocked off a shroom so easily enough to make a whole syringe) Within a few days a thick white growth, that kind of looked like spooge had taken over the entire jar. From this culture, the growth was transferred to several other jars of liquidy agar, all of which massively colonized and are displaying rhizomorphic growth.
Here is the problem, after getting a bunch of these jars the LC was injected into several PF / Rye flower style cakes, while at the same time spore solution was injected into several others of the same kind. This has been 5 days ago, the spore jars are showing massive colonizaton... Some might be finished within another few days, but those injected with the LC are showing no signs of life. What could have gone wrong?
3 days ago several jars of rye were also knocked up with the LC, those are showing no signs of life either... Something doesn't feel right here. Thank you, -An Anonymous phallus
grow mycelium out on agar long enough and it will suffer premature senility and eventually refuse to colonize... what kind of time are we talking about in terms of mycelium colonizing agar... or in your case, the cheap cooking-grade (from the sounds of it) agar medium. ;-p
Look into bacteriological grade agar for medium that remains solid up to 95(C)...
-------------------- Don't mistake my tone for a "matter-of-fact" attitude. I'm just presenting what I believe to be correct, until I'm corrected...