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 Cyber
Ash
  
Registered: 06/14/04
Posts: 1,422
Loc: Dearborn Michigan
Last seen: 8 days, 12 hours
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 Cloning questions 
#7421668 - 09/17/07 09:10 PM (4 years, 8 months ago) |
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I have had an issue with one strain. First off, it tends to produce sterile and mutant fruit. Not that I am complaining, the mutation tends to have some neat traits. Such as stalks that are 6" in diameter, 7" long, solid, with a cap that is about 6.5" in diameter. The also are STRONG! They blue with in moments of the slightest touch. So to start, if I clone from the mutant, will all the next batch be mutants, and if I clone from a good looking clump of fruit that produce good sporealation (is that a word?) and I clone from the fruit body, will the fruit from the next batch not have the mutant issue?
I guess I am asking can I select traits via cloning for propagation?
I remember reading some where that continued cloning of the fruit will produce mushrooms that no longer drop spores. Is this the case or is that an old wife's tale?
Is there a right and wrong way to clone?
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 36,974
Loc: USA Mountain Northwest
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Re: Cloning questions [Re: Cyber]
#7422553 - 09/18/07 05:50 AM (4 years, 8 months ago) |
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Normally, the traits in a fruit will carry over if you clone it. If you clone a mutant, you'll get harvests of mutants, provided the mutation was genetic in nature. If you clone from a cluster of large fruits, your future crops will develop clusters of large fruits.
Senescence is always a problem if you try to keep a fungal cell line going for too long. It isn't necessary though. Instead of constantly cloning the clones, clone the first one and then make a few master culture slants in test tubes to store the mycelium. You can then take it out of the refrigerator and use it for years to come without degradation.
RR
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www.mushroomvideos.com
semper in excretia sumus solim profundum variat
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Cyber
Ash
Registered: 06/14/04
Posts: 1,422
Loc: Dearborn Michigan
Last seen: 8 days, 12 hours
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Quote:
provided the mutation was genetic in nature.
What environmental factors could or would cause mutations in the fruit?
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 36,974
Loc: USA Mountain Northwest
Last seen: 14 hours, 9 minutes
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Re: Cloning questions [Re: Cyber]
#7441405 - 09/22/07 05:24 PM (4 years, 8 months ago) |
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Damage as the pins are forming, or for another example, damage from chemicals containing petroleum can be mistaken for mutations. I doubt there's an environmental condition you can easily create that would cause mutations.
RR
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www.mushroomvideos.com
semper in excretia sumus solim profundum variat
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 Captain Cubensis
Bleeding HeartLiberal
Registered: 09/18/07
Posts: 648
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I found that using too strong a peroxide mix causes mutations like upside down caps with gills on the top.
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www.groworganic.com
Get 350lbs shipped to your door for only $100!
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The Cyclestat 4P, a great repeat cycle timer made in the USA.
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Funkatron9000
The Teal Ninja
Registered: 10/03/07
Posts: 479
Loc: Under the stairs
Last seen: 1 year, 2 months
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lol, i'd like to see a upside down cap sometime. mabye i'll try a small batch and see if I can produce that sometime
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First Mono tub grow -R44- (w/pics)
  
I am the stone that the builder refused, I am the visual. The inspiration, that made ladys sing the blues.
I'm the spark that makes your idea bright. The same spark that lights the dark so that you can know your left from your right.
I am the ballot in your box, The bullet in the gun.
That inner glow, That lets you know to call your brother son.
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Captain Cubensis
Bleeding HeartLiberal
Registered: 09/18/07
Posts: 648
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Well, they were more like gills top and bottom, not really upside down, but with no smooth surface, all gills!
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www.groworganic.com
Get 350lbs shipped to your door for only $100!
www.mycosupply.com
Online Organic Rye Berries
www.hydroponics.net/i/200002
The Cyclestat 4P, a great repeat cycle timer made in the USA.
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rodfarva
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Registered: 07/31/07
Posts: 4,696
Last seen: 2 hours, 48 minutes
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Cyber, im in the D.. keep that in mind if u ever need to compair notes. (need suplies or trade possibly?)
At any rate im about to do my first attempt at cloning within a day or two. I already have two good LC's that have worked amazaing from a BRF cake biopsy (pixs) Im sure there is something i haven't thought of, still i just want to clone to have an easy reproductive source beyone the PITA of spore growth.
with that said, what i did was pop a needle into a BRF cake and inject the biopsy into my LC media. Is this technically a clone? I dont know. I have a master and a 2nd generation Lc (clone?) however they both are in baby bottles. Is there a reason you recoment test tubes rodger?
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