Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!
|
 Cyber
Ash
  
Registered: 06/14/04
Posts: 1,422
Loc: Dearborn Michigan
Last seen: 8 days, 12 hours
|
 Agar Questions 
#7453502 - 09/25/07 08:19 PM (4 years, 7 months ago) |
|
|
Ok, a few more agar questions.
First off I am really getting a kick out of the agar work and As you can tell, I have it working 
I have about 20 strains going and over 100 plates. I have isolated for rhizomorphic and fast growth and was wondering a few things.
First, when do you start to see sectioning? I have a few plates that are on there 4th transfer and I am still not seeing sectioning.
Example (Numbers indicate lineage 2-1-1-1 would be second streak plate first transfer, etc.)
The other question is, what is the percentage of sub-strains that will not fruit? I am only making 4 to 6 plates from my initial streak and trying to work them down to sectioning. Will I have enough to find a good strain?
Thanks
|
RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 36,974
Loc: USA Mountain Northwest
Last seen: 14 hours, 3 minutes
|
Re: Agar Questions [Re: Cyber]
#7454560 - 09/26/07 05:14 AM (4 years, 7 months ago) |
|
|
The bottom picture looks like a monoculture. Are these clones? If so, you won't see sectoring. I'd also suggest turning the dishes right side up.
RR
--------------------
www.mushroomvideos.com
semper in excretia sumus solim profundum variat
|
 orchidfanatic
retiree
Registered: 08/12/07
Posts: 832
Loc: where the wild things are
|
|
wow thats nice rhizomorphic growth on that second plate
|
Cyber
Ash
Registered: 06/14/04
Posts: 1,422
Loc: Dearborn Michigan
Last seen: 8 days, 12 hours
|
|
Quote:
RogerRabbit said:
The bottom picture looks like a monoculture. Are these clones? If so, you won't see sectoring. I'd also suggest turning the dishes right side up.
RR
RR, These are from spore not clones. On the second transfer or maybe it was the third, the dish was split in half. 1/2 was fuzzy growth the other was ryzomorphic growth. I only took from the ryzomorphic side as I was looking for ryzomorphic and fast growth.
By the bottom picture I must assume you are talking about 2-1-1-2. 2-1-1-1 is the same texture but is growing faster. What the heck, I may just do both and see how they do
|
RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 36,974
Loc: USA Mountain Northwest
Last seen: 14 hours, 3 minutes
|
Re: Agar Questions [Re: Cyber]
#7456574 - 09/26/07 04:10 PM (4 years, 7 months ago) |
|
|
Yes, you want to fruit them all. Don't just pick one. Save in the refrigerator a part of each dish you inoculate grains with, so when you determine the best performer(s), you'll have a fresh isolate ready to expand.
RR
--------------------
www.mushroomvideos.com
semper in excretia sumus solim profundum variat
|
fastfred
Old Hand
Registered: 05/17/04
Posts: 6,242
Loc: Dark side of the moon
|
|
Quote:
RogerRabbit said:
The bottom picture looks like a monoculture. Are these clones? If so, you won't see sectoring. I'd also suggest turning the dishes right side up.
RR
Kind of pointless to take pictures of the dishes with the agar side up. It is proper to write on the agar side though. It won't move around on you as the cover is rotated, so it's better to mark sections, etc..
Anyways I was curious to know your response to his question about what percentage of substrains are non-fruiters RR.
-FF
|
RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 36,974
Loc: USA Mountain Northwest
Last seen: 14 hours, 3 minutes
|
Re: Agar Questions [Re: fastfred]
#7457038 - 09/26/07 05:54 PM (4 years, 7 months ago) |
|
|
Very few rhizomorphic strains will be non-fruiting. I always ignore the cottony sectors anyway, so that ups the percentage. I won't even venture a guess as to percentage if you tried to fruit each sub-strain that differentiates.
We're looking for more than fruiting strains though. Average number of fruits per flush, weight of fruits, quality of fruits, resistance to aborting, etc., are all factors to consider when choosing a good strain to keep in master slants.
RR
--------------------
www.mushroomvideos.com
semper in excretia sumus solim profundum variat
| |
|
|
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Prisoner#1, RogerRabbit, EvilMushroom666
844 topic views. 2 members, 5 guests and 2 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic ]
| | |
|
|
|
Previous
Index
Next
Threaded
Shop for: 
Edit 
Reply 
