|
 jmg5
deadicated
Registered: 11/23/05
Posts: 635
Loc: miles above you
|
 bacteria and healthy mycelium? 
#7236779 - 07/29/07 05:18 PM (5 years, 9 months ago) |
|
|
has anyone here successfuly spawned jars contaminated with bacteria? I ask because in my experiments with liquid culture i've come upon a batch of jars with that characteristic sweet smell of bacteria, yet they still shake just fine and recolonize nearly all the way (some of the grains look wet).
I posted a couple days ago about bulk straw trays contaminating with cobweb while colonizing, have since determined the straw was not properly pasteurized for the amount of mold spores/dirt/who knows what else in the bale of straw. I've since spawned eight trays of straw - four with healthy jars and four with the contaminated jars. This time around the straw was completely pasteurized but was not washed or rinsed off prior to pasteurization.
the four healthy jars have produced four healthy trays now near 100% colonization
the other trays grew what looked like healthy mycelium throughout the straw, the bacterial odor was slight but still obviously present. The only visible contaminant was cobweb mold.
doing a quick search I found rr quoted as saying:
"Proper pasteurization will give you a two week window of opportunity to get the straw colonized to the point where the mushroom mycelium can hold its own against the contaminants"
I know it is possible to fruit mushrooms from a substrate contaminated with bacteria. Is it possible that this mycelium, already being contaminated (but not in a way that would prevent it from colonizing a substrate) was unable to defend itself against the mold spores already present in the straw? And is it possible that by washing and rinsing the straw before pasteurizing it I could get this bacterial myc to colonize all the way before I get cobwebs?
I'm just experimenting with these jars while i've got them, if nothing can come of them it doesn't really matter but I do plan on trying to fruit them in a seperate fruiting chamber if I could
thanks for reading
peace
|
ryanwgrad
MycoFanatic
Registered: 05/29/07
Posts: 215
Loc: New York
|
 Re: bacteria and healthy mycelium? [Re: jmg5]
#7236802 - 07/29/07 05:26 PM (5 years, 9 months ago) |
|
|
Yeah... the myc will spread even if it has a bac contam. I've had jars with sour rot where the myc spread just fine... the rot was still there though. What color is the contam? Pics? Or did you just smell it?
|
jmg5
deadicated
Registered: 11/23/05
Posts: 635
Loc: miles above you
|
Re: bacteria and healthy mycelium? [Re: ryanwgrad]
#7236963 - 07/29/07 06:32 PM (5 years, 9 months ago) |
|
|
no color in the jars, but like I said some of the grains appear slick the jars aren't so nasty that the grains clump together or stick to the jar when shaken, but they're far from being completely healthy
the pungent odor was the main indicator these jars were contaminated
Edited by jmg5 (07/29/07 06:35 PM)
|
ThePyschonaut52
Stranger
Registered: 04/24/07
Posts: 982
|
Re: bacteria and healthy mycelium? [Re: jmg5]
#7237049 - 07/29/07 07:09 PM (5 years, 9 months ago) |
|
|
yea. ive actually got 3 jars right now in my closet that are contaminated with "wet spot" and black mold. they still fully colonized, even with the contams.
--------------------
"In god we trust..."
-I guess we're screwed.
|
 RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 39,269
Loc: USA Mountain Northwest
Last seen: 2 days, 12 hours
|
|
But, it's highly unlikely you can fruit them indoors. Often, you can spawn them into some manure or straw in the ground and get a flush. RR
--------------------
Download Let's Grow Mushrooms
semper in excretia sumus solim profundum variat
|
ThePyschonaut52
Stranger
Registered: 04/24/07
Posts: 982
|
Re: bacteria and healthy mycelium? [Re: RogerRabbit]
#7237092 - 07/29/07 07:27 PM (5 years, 9 months ago) |
|
|
i usually destroy contaminated substrate, but this time im keeping them to observe what contams do.
--------------------
"In god we trust..."
-I guess we're screwed.
|
jmg5
deadicated
Registered: 11/23/05
Posts: 635
Loc: miles above you
|
Re: bacteria and healthy mycelium? [Re: RogerRabbit]
#7238196 - 07/29/07 11:46 PM (5 years, 9 months ago) |
|
|
i thought that might be a problem, I definitely wouldn't case them the layer would surely contaminate
|
|
Images only 
Threaded
Previous
Index
Next
Shop for: 
Edit 
Reply 
Anonymous
