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 TM
The Mind, The Many, The Music.
 
Registered: 06/11/02
Posts: 8,233
Loc: Under The Influence
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 Cloning Question
#714263 - 06/30/02 06:57 PM (9 years, 10 months ago) |
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I am attempting to clone PC (Cambodian). Using malt agar in 3 petri dishes innoculated with clean inner stem flesh and being kept at 88 degrees, the question is; How long before I should notice mycelial growth?
If the answer to this is in a FAQ, I couldn't find it.
If the answer to this is not finite, please give an estimate.
Finally, if this question does not belong here, I apologize in advance.
Thank you.
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"Have some congratulatory drugs." - C. Montgomery Burns
I'll probably always do drugs, so that just contributes to the addiction to The Shroomery... It's a vicious circle of bliss. 
TM™ 
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Champion des Champignons
long standing member;)
Registered: 07/26/00
Posts: 2,437
Loc: Alba
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 Re: Cloning Question [Re: TM]
#714764 - 06/30/02 10:59 PM (9 years, 10 months ago) |
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a week at the most, probably less..
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hmmm........
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 Xhulan
Stranger
Registered: 06/29/02
Posts: 13
Last seen: 9 years, 10 months
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Usually its very fast, taking around 72hours, but it can go up to a week depending on a lot of factors.
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A wise man once said : "Tune In, Turn on, Drop Out"
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zeronio
Stranger
Registered: 10/16/01
Posts: 2,349
Loc: Slovenia
Last seen: 7 months, 11 days
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Re: Cloning Question [Re: Xhulan]
#714878 - 07/01/02 01:28 AM (9 years, 10 months ago) |
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One factor can be peroxide. It takes longer if you use it.
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Champion des Champignons
long standing member;)
Registered: 07/26/00
Posts: 2,437
Loc: Alba
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 Re: Cloning Question [Re: zeronio]
#715013 - 07/01/02 03:44 AM (9 years, 10 months ago) |
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oops
true, true, I always use peroxidated agar, so don't know any better
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hmmm........
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tchyted
miestro
Registered: 09/03/01
Posts: 497
Loc: WA near seattle
Last seen: 7 years, 6 months
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Re: Cloning Question [Re: TM]
#715096 - 07/01/02 04:35 AM (9 years, 10 months ago) |
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i don't know about your particular strain, and i clone in licuid form, or directly onto substrate, not agar. in licuid, five days is typical, direct method, you will see hyphal fuzz within 24 to 48 hours, which should become obviously rizomorphic within five days typical. i always use peroxide.
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TM
The Mind, The Many, The Music.
Registered: 06/11/02
Posts: 8,233
Loc: Under The Influence
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No peroxide in my malt agar, so I should see some fuzz any minute now I guess, unless there's something wrong...
Thanks guys! You're the best!
--------------------
================================================
"Have some congratulatory drugs." - C. Montgomery Burns
I'll probably always do drugs, so that just contributes to the addiction to The Shroomery... It's a vicious circle of bliss.
TM™
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TM
The Mind, The Many, The Music.
Registered: 06/11/02
Posts: 8,233
Loc: Under The Influence
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All 3 dishes are showing nice healthy growth!! 5 days! 
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"Have some congratulatory drugs." - C. Montgomery Burns
I'll probably always do drugs, so that just contributes to the addiction to The Shroomery... It's a vicious circle of bliss.
TM™
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meatman
Goner
Registered: 04/22/01
Posts: 439
Loc: Underwater Lake
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Re: YeeHaa! [Re: TM]
#720288 - 07/03/02 10:44 AM (9 years, 10 months ago) |
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congrats! i hope everything turns out well for you. i'd like to see some pics if you've got a camera. if not, i just wanna know how it goes. g'luck
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"I don't do drugs. I am drugs." -Dali
Everything I post is a complete fabrication made for your entertainment.
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