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 MotorCityMadman
Rubberfan andFunketeer
Registered: 10/02/06
Posts: 202
Loc: Motown (State of Mind)
Last seen: 3 years, 6 months
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 peroxide dunk before tissue transfer? 
#7171172 - 07/13/07 03:15 PM (5 years, 10 months ago) |
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OK, apologies if this is old hat. I did search but couldn't reduce the clutter from searching on peroxide. on a slow connection and tor and that makes searching exceed the time-wasted-to-probability-of-annoying-other-shroomerites ratio unacceptably high.
Suppose you had a healthy bit of cloned tissue colonizing a moist piece of cardboard in a half pint jar. The jar started out sterile, but since cubes are so much happier on wood pulp than whatever might be floating by in the air, the only precaution has been an occasional quick mist of peroxide solution to keep the culture moist.
Continuing the hypothetical mental exercise, suppose you wished to transfer a piece of the (wholly imaginary) tissue to a clean jar of rye. Would you advise dunking the tissue sample in a peroxide solution inside a hypothetical glove box prior to snipping a bit of the tissue into said rye growing medium?
Also, one question about RogerRabbit (meant in a humorous tone): Did a bottle of Peroxide beat up your grandmother once or something? You seem to really hate the stuff! 
Thanks, comrades.
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 fastfred
Old Hand
Registered: 05/17/04
Posts: 6,585
Loc: Dark side of the moon
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Re: peroxide dunk before tissue transfer? [Re: MotorCityMadman]
#7171332 - 07/13/07 03:54 PM (5 years, 10 months ago) |
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Peroxide isn't effective against a lot of fungi and bacteria. It's usefulness is limited and it stunts the growth of healthy tissue.
There's only one way to do sterile culture and that is to practice sterile technique. Shortcuts only hurt you in the long run if you try to use them as your method.
To answer your question, I wouldn't use contaminated cultures at all. It's easy enough to do it again the right way. If you really want to use that culture then isolate it on agar first.
-FF
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CaptainLinger
A Fungus Amongus
Registered: 05/25/07
Posts: 1,756
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Re: peroxide dunk before tissue transfer? [Re: MotorCityMadman]
#7171676 - 07/13/07 05:14 PM (5 years, 10 months ago) |
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Yeah, he hates it because 1) EVERYONE gets worried about cobweb, and asks questions. Best way to treat it is with H202, and 2) It's not widely needed. The impression is somehow it's a magic sterilizer that doesn't impact mycelium and kills all contaminants; the opposite is more accurate.
It has very little use. Marginal help if there are very weak contaminants and strong mycelium.
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Terillius
Renaissance Man
Registered: 07/21/06
Posts: 1,190
Loc: UT
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Re: peroxide dunk before tissue transfer? [Re: MotorCityMadman]
#7172947 - 07/14/07 12:05 AM (5 years, 10 months ago) |
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That's nonsense. I always dunk my transfers to combat the bacteria that may escape my ghetto flowhood. It doesn't hurt the tissue one bit. It doesn't even slow it down.
I can't say with any weight that it helps significantly, but it doesn't hurt.
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Tamadragon
Stranger
Registered: 02/29/04
Posts: 382
Loc: CAN
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Re: peroxide dunk before tissue transfer? *DELETED* [Re: Terillius]
#7172956 - 07/14/07 12:07 AM (5 years, 10 months ago) |
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Post deleted by TamadragonReason for deletion: ===
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~Tama
Peace
I get real lonely
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 39,269
Loc: USA Mountain Northwest
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Re: peroxide dunk before tissue transfer? [Re: Tamadragon]
#7173070 - 07/14/07 12:34 AM (5 years, 10 months ago) |
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Dipping a culture into peroxide is a horrible idea before transfers. It does harm the tissue and sets it back right at the exact time you're wanting maximum rate of growth to outrun contaminants. Mold mycelium will recover from the peroxide dunk faster than the mushroom mycelium will, so you defeat your purpose.
You should transfer some of the culture to agar, so you can isolate the mycelium away from contaminants. I wouldn't even attempt to transfer non-sterile cardboard to grains. Simply work clean and sterile when you make the transfers. As soon as the mycelium starts to grow from the wedge, immediately transfer some of the new growth to new plates. Repeat(if necessary) until you have a clean culture. I've used peroxide when cloning heavily contaminated outdoor mushrooms that have been in my backpack for three days after picking, but that's a different story. It does set the tissue back significantly however.
RR
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Download Let's Grow Mushrooms
semper in excretia sumus solim profundum variat
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MotorCityMadman
Rubberfan andFunketeer
Registered: 10/02/06
Posts: 202
Loc: Motown (State of Mind)
Last seen: 3 years, 6 months
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Re: peroxide dunk before tissue transfer? [Re: RogerRabbit]
#7182681 - 07/16/07 12:04 PM (5 years, 9 months ago) |
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thanks everybody!
I think I have a better understanding of this issue now.
I've been avoiding agar since it's just another set of supplies that have to be surreptitiously obtained and stored.
is there a stealth agar "tek"? 
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 39,269
Loc: USA Mountain Northwest
Last seen: 2 days, 16 hours
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Re: peroxide dunk before tissue transfer? [Re: MotorCityMadman]
#7184997 - 07/16/07 10:29 PM (5 years, 9 months ago) |
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No real need to be stealth with agar. Just grow some edibles such as lion's mane or oyster that grow well on pf tek. As long as it's just a hobby and you can give anyone who finds out something to cook in the kitchen, it's all good.
RR
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Download Let's Grow Mushrooms
semper in excretia sumus solim profundum variat
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jeanpignon
frenshchampignon
Registered: 08/07/07
Posts: 115
Loc: my mind,
Last seen: 1 year, 1 month
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Re: peroxide dunk before tissue transfer? [Re: RogerRabbit]
#7276792 - 08/09/07 04:00 AM (5 years, 9 months ago) |
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in fact, peroxide do kill any kind of spore.
also our loved mush spore.
but it doesn't kill existing fungy culture whatever they are.
you can use peroxide to protect clean tissue... i meen CLEAN tissue,
with a concentration of 0.15% pure peroxide(10% of the 3%pero.mix).
for straw or hey you can also pasteurise it without heating with the
same concentration like rush wayne says in his manual.
peroxide in the right concentration is an helpfuul tool, believe me.
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bonjour les shroom!!!
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