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 LiquidMartyr
Newbie
Registered: 05/13/05
Posts: 5
Last seen: 4 days, 8 hours
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Whatsup everyone. This is my first post on the shroomery. Just want to say that I love everything about this site. It has been extremely helpful.
Let me tell you about this dream I had in regards to the Karo TEK..... I used the Ziplock round, medium "Twist,n lock" containers. I filled them 750 ML of water and about 5 TSP of karo. I used about a square foot of saran wrap when I boiled them (no top). I microwaved them for 6 minutes with no melting problems. When they stopped boiling i put a rubberband around the base of the threads to secure the saran wrap. When they cooled I inoculated one of eatch (they come 2 in a pack... $3.75)with Treasure Coast and Ecuador spores, and then put the tops on. I did it about an hour ago. I guess I will find out in about a week. Hopefully, this will help someone out. Thanks everyone.
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Yeah, I did that.
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down44
Johnson
Registered: 12/02/04
Posts: 177
Last seen: 3 months, 1 day
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awesome tek thanks!
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 shymanta
Mad Scientist
Registered: 01/27/05
Posts: 904
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Quote:
my friend followed these procedures only he put in slightly more syrup, and after nuking the liquid turned a light tint of yellow... is that to be expected?
This may be the sugars caramelizing. Reduce your heat time. In a pressure cooker it should only be about 30 minute. I don't know about a microwave. Maybe heat more times for a shorter period with a rest in between?
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FooMan
Registered: 02/02/05
Posts: 8,278
Loc: Earth 
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 Re: Super easy karo tek  [Re: shymanta]
#5125380 - 12/31/05 09:33 AM (7 years, 4 months ago) |
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Quote:
shymanta said:
Quote:
my friend followed these procedures only he put in slightly more syrup, and after nuking the liquid turned a light tint of yellow... is that to be expected?
This may be the sugars caramelizing. Reduce your heat time. In a pressure cooker it should only be about 30 minute. I don't know about a microwave. Maybe heat more times for a shorter period with a rest in between?
LOL, Check out the date on that post Shymanta. I would hope after nearly 2 years that they figured the answer out by now!
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ShroomArtist84
Stranger
Registered: 08/09/05
Posts: 2,414
Last seen: 7 years, 3 months
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Re: Super easy karo tek [Re: FooMan]
#5125432 - 12/31/05 10:01 AM (7 years, 4 months ago) |
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great post man!!!!
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No matter what I say and no matter what I write here.
I'm sick of always looking at this page with a blank stare.
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shymanta
Mad Scientist
Registered: 01/27/05
Posts: 904
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Damn, that's funny. Didn't even see the date. I sure hope they figured it out. Maybe someone else will get something from it.
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Snaggletooth
Stranger in a Strange Land
Registered: 10/24/05
Posts: 6,109
Loc: blinks stupidly
Last seen: 3 years, 4 months
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Re: Super easy karo tek [Re: shymanta]
#5126018 - 12/31/05 03:03 PM (7 years, 4 months ago) |
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shymanta: gets the necromancy award of the day 
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Atheist Chat
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Spacedone
Stranger
Registered: 08/18/06
Posts: 29
Last seen: 6 years, 6 months
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Re: Super easy karo tek [Re: P2K1]
#6263668 - 11/09/06 12:28 AM (6 years, 6 months ago) |
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a quick question
do you have to suck up the white mycellum itself or will the liquid itself be able to innoc with
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oso
man
Registered: 09/27/06
Posts: 158
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Re: Super easy karo tek [Re: FooMan]
#6263696 - 11/09/06 12:42 AM (6 years, 6 months ago) |
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haha.
Quote:
FooManShroom said:
LOL, Check out the date on that post
but yea, you are aiming for the myc, not the sugar water.
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"When tyranny and oppression come to this land, it will be in the guise of fighting a foreign foe."
James Madison
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 jack_straw2208
Doctor
Registered: 02/12/07
Posts: 870
Loc: yo mamma's house
Last seen: 1 minute, 43 seconds
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Re: Super easy karo tek [Re: Spacedone]
#6643798 - 03/07/07 02:06 AM (6 years, 2 months ago) |
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Quote:
Spacedone said:
a quick question
do you have to suck up the white mycellum itself or will the liquid itself be able to innoc with
bump
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I'm pullin' steel wires out of my eyes
they're 20 miles long tangled up with my all insides
DieCommie said:
cut off her nose to spider face
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djnoktirnal
Ponderer
Registered: 04/26/06
Posts: 195
Loc: the psychabyss
Last seen: 1 year, 10 months
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Quote:
jack_straw2208 said:
Quote:
Spacedone said:
a quick question
do you have to suck up the white mycellum itself or will the liquid itself be able to innoc with
bump
i would try to suck up myc, although it's probably throughout the water even though you can't see it
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WWJDWWMD's?
What Would Jesus Do With Weapons of Mass Destruction?
  
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Blutjager
Inhuman
Registered: 06/12/06
Posts: 9,220
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YES you need to suck up the mycelium
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Cake makers please read this before you waste more time/effort >>
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/6236058/an/0/page/0
My LC tek >> http://www.shroomery.org/forums/showflat.php/Cat/0/Number/7320516/an/0/page/0
""THEY CAN NOT KILL ME,I STILL WALK THE EARTH,WEAKENED BUT ALIVE.THEY HAVE TAKEN ALL FROM ME BUT THE BEATING OF MY COLD DEAD HEART.
THEY WILL NOW LEARN OF FEAR,THESE MEWING BABIES WHO THOUGHT THEY COULD DESTROY ME.
I HAVE REGAINED MY POWERS AND SHALL COME TO THEM IN THE NIGHT........
MY VISITS SHALL NOT BE KIND !! ""
 BlutJager
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figgusfiddus
Arrogant Worm
Registered: 02/02/07
Posts: 2,126
Loc: Figgus, Fiddia
Last seen: 4 years, 7 months
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Re: Super easy karo tek [Re: Blutjager]
#6647137 - 03/08/07 12:35 AM (6 years, 2 months ago) |
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Sucking up mycelium isn't hard, if that's what the previous poster was wondering. This is really fluffy stuff, it's only going to even be visible because it's so bloated with water.
I've done simple, successful tissue cultures by just tossing a hunk of stalk in the processor with sterile water, sucking whatever you can up and inoculating. Doing it THAT way will jam up your blunt needle sometimes, but you can just shoot it back out and try again until it works. That's your worst-case scenario with this myc... but I don't think you'll run into that at all.
That said, even the karo water, as long as you let it colonize and then shake it up nicely, is full of tissue and cells. It's just that you get way more tissue if you suck up the actual mycelium. I did experiment a little, by sucking up the really scummy, thick tissue for one jar and just the clearish water for the next, and though the scummy jar got off to a faster start, in the end colonization times don't seem to differ more than one two days (didn't do enough jars in that batch to give a nice scientific average). I figure that has to do with the fact that the watery culture will disperse the tissue over a larger area, making up for some of the lost time, but that's a straight-from-the-ass hypothesis.
Edit: On second thought it should be said that whether you absolutely have to suck up visible mycelium probably has a lot to do with how much it's colonized by the time you start extracting. Like the original poster said though, if you shake it up, you've got 200mL of inoculant (or however much you make), not just the 20mL or so that has visible mycelium in it.
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FGSFDSFGSFDSFGSFDSFGSFDSFGSFDS
FGSFDSFGSFDSFGSFDSFGSFDSFGSFDS
FGSFDSFGSFDSFGSFDSFGSFDSFGSFDS
Edited by figgusfiddus (03/08/07 12:43 AM)
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losfreddy
Composter
Registered: 02/16/04
Posts: 4,027
Last seen: 16 minutes, 59 seconds
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wow that a very good one. I was uding the half pint ziplock containers with a burnt hole and electirc tape. seems like a bet.
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Quit reading my mind!
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figgusfiddus
Arrogant Worm
Registered: 02/02/07
Posts: 2,126
Loc: Figgus, Fiddia
Last seen: 4 years, 7 months
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Re: Super easy karo tek [Re: losfreddy]
#6652456 - 03/09/07 12:52 PM (6 years, 2 months ago) |
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Just a question about applying this karo tek (or any karo tek) to inoculation with a questionable spore solution:
If I'm not sure my spore solution is very clean, can I apply this tek, then add a little h2o2 before using the karo solution as an inoculant?
As I understand h2o2, it won't hurt live mycelium in small amounts, but it will kill mold spores and bacteria left and right. Thus, by growing live mycelium in a karo solution, it would seem to me you could "clean up" your spores by allowing them to germinate before killing off their competition. I'm pretty sure none of the mold spores can produce advanced enough structures in this time to survive the peroxide holocaust.
Thoughts? I've seen people use h2o2 to clean up messy clones, but I haven't read about anyone using it to effectively clean up bad spores by allowing them to germinate.
Edit: Never mind, I just read this exact same thing in the other karo tek. So uh, I guess I don't get genius credit.
Edited by figgusfiddus (03/11/07 09:31 PM)
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