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 agar
old hand
Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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 TURBINADO LC
#6378527 - 12/17/06 02:24 AM (1 year, 8 months ago) |
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PC'ed 12 minutes.
Very clear & just slightly gold tinted.
(no sediment)
Will inoculate after it's cooled.
And, see how it works.
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 etam
it's a hobby...
Registered: 04/24/06
Posts: 1,123
Loc: East Coast
Last seen: 1 month, 14 days
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Re: TURBINADO LC [Re: agar]
#6378598 - 12/17/06 03:26 AM (1 year, 8 months ago) |
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This will be interesting.
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JonnyDeformed
ॐ
Registered: 03/20/06
Posts: 1,296
Loc: Directlyundertheearthssun...
Last seen: 11 hours, 38 minutes
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Re: TURBINADO LC [Re: etam]
#6378612 - 12/17/06 03:41 AM (1 year, 8 months ago) |
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If this works then there would be no reason why my experiment idea won't. I've been thinking about spawning to sugar cane mulch since i have a pile. What do you think?
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Me & Morph
Australian Ethnobotany ॐ
Triple J UnEarthed Music Competition
´¯`ˇ.¸¸.ˇ´¯Life itself is only a vision, a dream. nothing exists save empty space and you, and you are but a thought..¸¸.ˇ´¯`ˇ.¸¸
Edited by JonnyDeformed (12/17/06 03:42 AM)
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ohmatic
searcher
Registered: 02/28/04
Posts: 4,884
Loc: europe
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hey agar, just cause i spot this lc post.
about the longterm storage of liquid myc, iirc you mentioned (once the lc is ready to use)
to use sterile needles to get out as much LIQUID (not myc) as possible.
then squirt in sterile water (and maybe wait some days till the myc digested
any still remaning nutes)
draw a syringe with myc and squirt into lil jar filled with sterile water -> refrig.
it was about that, wasit ?
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 MONOTUB tek  HEATBOMB tek
#cultivation on irc.shroomery.org for real time cultivation chat
stay a minimium of 10 minutes after asking a question if you
actually expect someone to read and answer it !!!
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agar
old hand
Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: TURBINADO LC [Re: ohmatic]
#6378639 - 12/17/06 04:30 AM (1 year, 8 months ago) |
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For long term storage of mycelium from an LC.
Best bet is to inject into a test tube, w/port or rubber stopper.
Or, a vacutainer.
Then stand the tube upright.
So the mycelium will settle to the bottom.
Then, aspirate out as much liquid as possible.
Then, inject sterile distilled water.
Object is to remove as much nutrient as possible.
Then, refrigerate the tubes.
Lacking air, nutrients & refrigerated.
Mycelium in liquid suspension will store for a considerable time.
Another method RR brought to light.
Is to colonize a pint or quart jar of wbs/grains.
But, have jar only 1/4 to 1/3rd full of spawn.
Then, inject sterile distilled water.
Shake the jar violently.
(to dislodge mycelium from wbs/grains).
Then, aspirate out mycelium into syringes.
For long term refrigerated storage.
That way, there is no nutrients in the syringe.
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Blutjager
Inhuman
Registered: 06/11/06
Posts: 8,240
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Re: TURBINATE LC [Re: agar]
#6378753 - 12/17/06 07:46 AM (1 year, 8 months ago) |
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I always see those little packets of "Raw" sugar,I look forward to see how this pans out
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Cake makers please read this before you waste more time/effort >>
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/6236058/an/0/page/0
My LC tek >> http://www.shroomery.org/forums/showflat.php/Cat/0/Number/7320516/an/0/page/0
""THEY CAN NOT KILL ME,I STILL WALK THE EARTH,WEAKENED BUT ALIVE.THEY HAVE TAKEN ALL FROM ME BUT THE BEATING OF MY COLD DEAD HEART.
THEY WILL NOW LEARN OF FEAR,THESE MEWING BABIES WHO THOUGHT THEY COULD DESTROY ME.
I HAVE REGAINED MY POWERS AND SHALL COME TO THEM IN THE NIGHT........
MY VISITS SHALL NOT BE KIND !! ""
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Blutjager
Inhuman
Registered: 06/11/06
Posts: 8,240
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How many packets did you put into how much water ??
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Cake makers please read this before you waste more time/effort >>
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/6236058/an/0/page/0
My LC tek >> http://www.shroomery.org/forums/showflat.php/Cat/0/Number/7320516/an/0/page/0
""THEY CAN NOT KILL ME,I STILL WALK THE EARTH,WEAKENED BUT ALIVE.THEY HAVE TAKEN ALL FROM ME BUT THE BEATING OF MY COLD DEAD HEART.
THEY WILL NOW LEARN OF FEAR,THESE MEWING BABIES WHO THOUGHT THEY COULD DESTROY ME.
I HAVE REGAINED MY POWERS AND SHALL COME TO THEM IN THE NIGHT........
MY VISITS SHALL NOT BE KIND !! ""
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TurdFerguson
Folk Villain
Registered: 07/02/05
Posts: 1,311
Loc: 123 Fake St.
Last seen: 5 months, 28 days
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Re: TURBINADO LC [Re: agar]
#6378869 - 12/17/06 09:28 AM (1 year, 8 months ago) |
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Quote:
agar said:
Lacking air, nutrients & refrigerated.
Mycelium in liquid suspension will store for a considerable time.
What would a considerable amount of time be, relatively? Is there a ballpark window of time you have discovered before they would expire?
Thanks,
DD
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Hey, baby...wanna kill all humans?
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total
Dr Shroom
Registered: 10/03/04
Posts: 1,061
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well dickdiver...id say at least a yr...iv heard of longer when stored on slants though...
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I Need Glass Petri's
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Vibrate
with me
Registered: 11/18/06
Posts: 252
Last seen: 3 hours, 47 minutes
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Convenient premeasured sugar packets. If it works out, I always like convenience. Good luck.
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I love you
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agar
old hand
Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: TURBINATE LC [Re: Vibrate]
#6380056 - 12/17/06 05:28 PM (1 year, 7 months ago) |
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I used a 5 gram packet, into about 220 ml of distilled water.
Heated the jar of water in the microwave.
Dumped in the packet content & stirred it.
To dissolve the little crystals.
Then, added a lid & PC'ed.
Cooled overnight, inoculated with about 2 ml spore solution.
No stir bar.
Incubated around 75F a few days.
 
Small myc cloud is developing.
(hard to photograph)
Object was to just see if it would germinate/colonize.
Will test more & adjust for optimal solution.
$0.69 cent box contains 25 packets @ 5 grams each.
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total
Dr Shroom
Registered: 10/03/04
Posts: 1,061
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Re: TURBINATE LC [Re: agar]
#6380096 - 12/17/06 05:38 PM (1 year, 7 months ago) |
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good job agar...sugar in the raw...would of never thought of that...reminds me of the time i was a n00b and tried to use table sugar as an additive in my agar!! 
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I Need Glass Petri's
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Hypercube
PWsteal.Ldpinch.D
Registered: 12/18/05
Posts: 634
Loc: Crab Nebula
Last seen: 9 hours, 41 minutes
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Re: TURBINATE LC [Re: total]
#6380993 - 12/17/06 08:24 PM (1 year, 7 months ago) |
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Good to see this works!!
I'll try this instead of honey next time, have loads of raw sugar hanging around.
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See you there.
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kjb1891
Shroom Dreamer
Registered: 07/17/02
Posts: 171
Last seen: 1 month, 8 days
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Re: TURBINADO LC [Re: agar]
#6383926 - 12/18/06 05:13 PM (1 year, 7 months ago) |
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What about adding something like gelatin in with the sugar for some amino acids?
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agar
old hand
Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: TURBINADO LC [Re: kjb1891]
#6383959 - 12/18/06 05:20 PM (1 year, 7 months ago) |
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Quote:
kjb1891 said:
What about adding something like gelatin in with the sugar for some amino acids?
Nope, not a good idea.
Think about it.
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agar
old hand
Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: TURBINADO LC [Re: kjb1891]
#6385309 - 12/19/06 03:23 AM (1 year, 7 months ago) |
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Primate
former addict
Registered: 08/12/99
Posts: 855
Last seen: 3 months, 19 days
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Re: TURBINADO LC [Re: agar]
#6385394 - 12/19/06 05:10 AM (1 year, 7 months ago) |
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Quote:
agar said:
  
VERY NICE! and bookmarked.
you are truly an innovator Agar
Much love.
-Prim
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"Power to the peaceful"
-Micheal Franti and Spearhead
Edited by Primate (12/19/06 05:12 AM)
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agar
old hand
Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: TURBINADO LC [Re: Primate]
#6385401 - 12/19/06 05:13 AM (1 year, 7 months ago) |
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LOL, this was the first go.
The one I started with.
5 gram packet to about 220 ml distilled water.
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VampireSlayer
killing ghosts,zombies andvampires forlife
Registered: 08/26/06
Posts: 2,529
Last seen: 10 months, 20 days
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Re: TURBINADO LC [Re: agar]
#6385414 - 12/19/06 05:22 AM (1 year, 7 months ago) |
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nice! turbinado sugar ftw! now we don't have to mess with sticky syrup anymore!
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I Don't come to fight flesh and blood but spiritual wickedness in high and low places
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tahoe
Noob Slayer
Registered: 11/26/03
Posts: 4,506
Loc: N38.93829W119.98108
Last seen: 30 days, 5 hours
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awesome work agar, ths will be my new lc additive once this bag of tan malt turns into a rock
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Alder is not the BEST type of to use
Heat drying cubes is okay and recommended
Sunflower seeds do not cause contams
You should directly mist your cakes
The subjects that I cover are not species specific and can be used in All Aspects of Mycology
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Oatman2000
-=Outa Space=-
Registered: 05/10/05
Posts: 2,662
Loc: Planetary Nebula
Last seen: 20 hours, 58 minutes
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Re: TURBINADO LC [Re: tahoe]
#6387851 - 12/19/06 07:38 PM (1 year, 7 months ago) |
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wow this is great and cheap! i have bookmarked this page also.
will be adding a link in my journal also. Truly TRULY! a master of your hobby!
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 Spawning to COIR
My Chocolate Recipe
WBS QUART SPAWN JAR PREPERATION
----------------------------
4-PO-DMT; 4-phosphoryloxy-N,N-dimethltryptamine
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tahoe
Noob Slayer
Registered: 11/26/03
Posts: 4,506
Loc: N38.93829W119.98108
Last seen: 30 days, 5 hours
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The lc's i made with this stuff is looking great!! Nice and clear liquid. I like it better then malt. Pics to come
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Alder is not the BEST type of to use
Heat drying cubes is okay and recommended
Sunflower seeds do not cause contams
You should directly mist your cakes
The subjects that I cover are not species specific and can be used in All Aspects of Mycology
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Sillicybin
Registered: 02/14/05
Posts: 1,431
Loc: TX (but don't stereotype!...
Last seen: 7 hours, 15 minutes
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Re: TURBINADO LC [Re: tahoe]
#6427869 - 01/05/07 12:35 PM (1 year, 7 months ago) |
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Quote:
tahoe said:Pics to come
.....?
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RogerRabbit said:
"Rush Wayne's peroxide tek is crap. There's no other words for it, and I've been saying it for years. It's right up there with 'fractional sterilization' and the 'oven tek'."
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FGL
Stranger
Registered: 06/25/05
Posts: 555
Last seen: 3 hours, 28 minutes
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Where I live I cannot find "sugar in the raw" what kind of sugar should I look for?
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Make your own Magnetic Stirrer for L.C ( Several designes Links)
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mattymonkey
Feel Like aStranger...
Registered: 11/07/04
Posts: 973
Last seen: 9 days, 1 hour
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Re: TURBINADO LC [Re: FGL]
#6598711 - 02/22/07 06:10 PM (1 year, 5 months ago) |
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i assume any raw cane sugar should work fine
is there anything special about "turbinado"? other then it comes in packets perhaps..
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"listening for the secret.. searching for the sound.."
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RoachMan
LOOK! I FingeredIt Out!
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