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 Bloc
Psychonaut
 
Registered: 01/06/06
Posts: 208
Last seen: 9 days, 8 hours
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 Possible experiment for Ozone Substrate sterilization. 
#6153515 - 10/10/06 08:37 AM (5 years, 7 months ago) |
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Ok, I'm know there are many posts on this, but I wanted to put my 2 cents.
Building an Ozone Generator is not difficult. Just a handful of components.
I've been thinking how one would sterilize a substrate with it. Here is my plan.
In the case of BSF and verm, one could use a FoodSaver device and vacuum seal the jars. Then you can transfer the jars to a glovebox that has a ozone generator inside, saturating the air inside with ozone. Once the jars are in and the air is filled with ozone, you can then lift the lids, flooding the jars with ozone.
Since microbes are much much larger then atoms (duh :P) the air would reach every nook and cranny inside the substrate. My thinking is that if air cant reach it, there cant be anything alive there. So the Ozone would get everything.
One can argue that the water might sheild some of the contaminates, but I don't think this is the case. A flim of water around a microbe will also oxidize, and it is known that pouring ozonated water over a surface will kill everything on it. 
I am going to try this, but i wanted some input. Anyone have anything to add or suggest? 
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 BIGSWANG
oakridge gang, beotch
Registered: 02/18/02
Posts: 18,678
Loc: Iwishanigga woods
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 Re: Possible experiment for Ozone Substrate sterilization. [Re: Bloc]
#6153530 - 10/10/06 08:41 AM (5 years, 7 months ago) |
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correct me if im wrong but ozone is just o3 ...and to my knowledge ....it is used to get smells out of the air...
i dont think o3 is a sterilizer..or would work in your experiment....if you really want to test it...get some mold going on some agar..pop it in the glove box filled with o3..and im pretty sure the mold will survive...
let me know what happens
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Admin Edit: Your signiture is inappropriate and has been removed. Do not harass other members in your signiture. Also refrain from posting links to scat pornography. If I see anything like that here again, you will be banned.
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Bloc
Psychonaut
Registered: 01/06/06
Posts: 208
Last seen: 9 days, 8 hours
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Re: Possible experiment for Ozone Substrate sterilization. [Re: BIGSWANG]
#6153576 - 10/10/06 08:59 AM (5 years, 7 months ago) |
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Well, Ozone IS used as a purifyer because it kills all single cell microbes
Quote from wiki
Industrially, ozone or ozonated water is used to
disinfect water before it is bottled;
deodorize air and objects, such as after a fire;
kill bacteria on food or on contact surfaces;
scrub yeast and mold spores from the air in food processing plants;
wash fresh fruits and vegetables to kill yeast, mold and bacteria;
So it Can be used for this. The trick is the saturation.
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BIGSWANG
oakridge gang, beotch
Registered: 02/18/02
Posts: 18,678
Loc: Iwishanigga woods
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Re: Possible experiment for Ozone Substrate sterilization. [Re: Bloc]
#6153661 - 10/10/06 09:27 AM (5 years, 7 months ago) |
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hmmm...never heard of this...post some updates when you try it out....
putting an ozone generator in a sealed tub for a couple of hours will def saturate the air with o3 ...try it out with an agar slab like i suggested..tell us what happens
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Admin Edit: Your signiture is inappropriate and has been removed. Do not harass other members in your signiture. Also refrain from posting links to scat pornography. If I see anything like that here again, you will be banned.
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