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 Tippinthru
contented
Registered: 04/07/05
Posts: 1,131
Loc: "The Garden"...
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 Re: To Magnetic Stirrer Users'  [Re: HippieChick]
#5540608 - 04/21/06 09:35 AM (6 years, 1 month ago) |
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http://www.shroomery.org/forums/showflat...rue#Post4244978
Check out Agar's stir bar method...works for me! Just watch the Garbage Disposal Problem! LOL!
Nice find HC and at a good price too! I  working with LC!
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Perfection is attained by slow degrees; it requires the hand of time...
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 IGnosticAbhorI
Stranger-er
Registered: 11/06/04
Posts: 4,898
Last seen: 2 years, 6 months
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 Re: To Magnetic Stirrer Users' [Re: HippieChick]
#5540816 - 04/21/06 10:52 AM (6 years, 1 month ago) |
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Quote:
 
That's what i'm use to seeing 
-Gnostic
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Blue Helix
old hand
Registered: 02/02/03
Posts: 1,095
Last seen: 1 day, 14 hours
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Fast speeds that make a visible vortex are neither required nor will speed up the colonization. In Paul Stamet's book Growing Gourmet & Medicinal Mushrooms (page 149) he recommends only 100 to 200 RPM, and that would not be fast enough to induce a vortex except maybe a small dimple in the surface. It certainly wouldn't look anything like the pictures above.
I like to run mine slow at first, especially with spores, so that the liquid just turns and shows no vortex at all. Once the mycelium globs start to form, I turn it up a little so that the magnet can shear the large blobs. At those speeds, I only see a little dip on the surface, not a vortex. Here is a picture showing what it looks like (note the dimple on the surface):
I only turn the speed high enough for a vortex if the mycelium is sticking to the walls, magnet, or surfaces to dislodge them, then I turn it back down after a minute or two.
Having said all of that, I have run a LC with a vortex nearly half way down the liquid the entire time and was just fine. If you do run fast enough to make a big vortex like in those pictures above, just keep in mind that the more motion you invoke, the more heat will be generated by the movement. And potentially even worse, some units run hotter on the plate itself when the RPMs are increased which will heat up the liquid even more, so always keep the temperature of the LC in check when you run fast. The rule is that no matter what speed, you don't want the LC over the mid 80s for cubensis because as the temperature rises, the mycelium slows down and the bacteria, if any, will have the upper hand.
Edited by Blue Helix (04/26/06 12:08 AM)
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Hippie3
mycotopiate
Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
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Re: To Magnetic Stirrer Users' [Re: Blue Helix]
#5557636 - 04/26/06 05:00 AM (6 years, 1 month ago) |
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for the do-it-yourself crowd-
build a magnetic stirrer
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Admin @ mycotopia.net
Mycotopia
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FooMan
Libertarian
Registered: 02/02/05
Posts: 7,534
Loc: Earth 
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Re: To Magnetic Stirrer Users' [Re: HippieChick]
#5557655 - 04/26/06 05:10 AM (6 years, 1 month ago) |
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Nice new toy there HC! 
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Oatman2000
-=Outa Space=-
Registered: 05/10/05
Posts: 2,860
Loc: Planetary Nebula
Last seen: 6 days, 18 hours
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Re: To Magnetic Stirrer Users' [Re: FooMan]
#5557771 - 04/26/06 06:46 AM (6 years, 1 month ago) |
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Hc - I was told not to substitute Malto Dextrin for Dextrose??!?
Dextrose, commonly called glucose, d-glucose, or blood sugar, occurs naturally in food, and is moderately sweet. It is a monosaccharide (basic unit of carbohydrates, C6H1206) and has a high glycemic index (digested carbohydrates ability to raise blood glucose levels, also called Gl) ranking at 100.
Maltodextrin is a sweat, easily digested carbohydrate made from cornstarch. The starch is cooked, and then acid and/or enzymes (a process similar to that used by the body to digest carbohydrates) are used to break the starch into smaller chains (3-20 chains in maltodextrin). These chains are composed of several dextrose molecules held together by very weak hydrogen bonds.
To clarify, carbohydrates are molecules of carbon, hydrogen, and oxygen produced by plants through photosynthesis. The term saccharide is a synonym for carbohydrate; a monosaccharide (mono=1) is the fundamental unit of carbohydrates. Disaccharides (Di=2) are molecules containing 2 monosaccharide units. Di and monosaccharides are also known as sugars, simple sugars, or simple carbohydrates. Next are oligosaccharides, and polysaccharides. Oligosaccharides are made of 3-9 monosaccharide links. Polysaccharides consist of 10 to thousands of monosaccharide links. A complex carbohydrate refers to many monosaccharide units linked together. In addition, you will often hear the terms ?long?, and ?short? carbohydrate chains. Short carbohydrate chains are those under 10 sugar molecules. And long chains are those over 10 sugar molecules. Which fits in conjunction with the above terms, Oligosaccharides and Polysaccharides.
Dextrose is labeled a simple carbohydrate and Maltodextrin complex. And now this should make perfect sense. But don?t be fooled by the word, ?complex.? The bonds that compose maltodextrin are very weak, and readily broken apart in your stomach; moreover, the chain is extremely minimal in composition. The weak bonds, and fragile composition of maltodextrin cause it to be digested a fraction slower than dextrose. Why this is so and what exactly hydrogen bonds are will be assessed subsequently.
have you sucessfully made a LC with it?
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 Spawning to COIR
My Chocolate Recipe
WBS QUART SPAWN JAR PREPERATION
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4-PO-DMT; 4-phosphoryloxy-N,N-dimethltryptamine
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,064
Loc: my room
Last seen: 2 days, 1 hour
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Re: To Magnetic Stirrer Users' [Re: Blue Helix]
#5558069 - 04/26/06 09:27 AM (6 years, 1 month ago) |
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>he recommends only 100 to 200 RPM, and that would not be fast enough to induce a
>vortex except maybe a small dimple in the surface.
Indeed.
This video shows my stirrer at 120 1/min.
The jar on the right is placed where it is supposed to be placed, the other 2 jars are placed offsite, but still stirring. This makes it possible to place 9 jars on it!
http://www.youtube.com/watch?v=DFwXqkYpML0
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Cubenisseur
Mad Props
Registered: 12/04/05
Posts: 1,392
Loc: Indian Land
Last seen: 2 years, 1 month
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Re: To Magnetic Stirrer Users' [Re: Anno]
#5558157 - 04/26/06 09:55 AM (6 years, 1 month ago) |
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Nice. Smart thinking on that one.
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HippieChick
Chicks can do it too!
Registered: 02/20/05
Posts: 5,821
Loc: Midwest
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Re: To Magnetic Stirrer Users' [Re: Oatman2000]
#5558186 - 04/26/06 10:02 AM (6 years, 1 month ago) |
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I've made 50-60 with it
That bag is almost gone
Peace,Love,Happiness and Harmony
Hippie Chick 
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Freedom's just another word for nothing left to lose...
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Oatman2000
-=Outa Space=-
Registered: 05/10/05
Posts: 2,860
Loc: Planetary Nebula
Last seen: 6 days, 18 hours
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Re: To Magnetic Stirrer Users' [Re: HippieChick]
#5558220 - 04/26/06 10:09 AM (6 years, 1 month ago) |
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sweet... but a little too late.
Just ordered dextrose from sporeworks, along with some new strains.
but i know what i'm usin from now on...
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Spawning to COIR
My Chocolate Recipe
WBS QUART SPAWN JAR PREPERATION
----------------------------
4-PO-DMT; 4-phosphoryloxy-N,N-dimethltryptamine
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