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 Mobius_Strip
Distant Relative
Registered: 03/11/05
Posts: 322
Loc: Spangladesh
Last seen: 5 years, 10 months
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 Need help isolating strains 
#5414255 - 03/17/06 05:48 PM (6 years, 2 months ago) |
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I have two agar wedges of pan goliath and I'm not quite sure which strains to isolate from here. I know the fuzzy ones arent ideal but the small blotchy areas don't seem ideal either. I'm unsure about this. Can anyone point me in the right direction?
I know the pictures arent great but it's the best I can do without a tripod.
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The smart way to keep people passive and obedient is to strictly limit the spectrum of acceptable opinion, but allow very lively debate within that spectrum - even encourage the more critical and dissident views. That gives people the sense that there's free thinking going on, while all the time the presuppositions of the system are being reinforced by the limits put on the range of the debate
-Noam Chomsky
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 bluemeanie
Cow boy
Registered: 06/18/01
Posts: 5,716
Loc: orbit
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Re: Need help isolating strains [Re: Mobius_Strip]
#5414294 - 03/17/06 06:04 PM (6 years, 2 months ago) |
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of areas and just see how they grow out.
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Holidaying in San Diego...
Edited by bluemeanie (02/02/07 02:46 AM)
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Mobius_Strip
Distant Relative
Registered: 03/11/05
Posts: 322
Loc: Spangladesh
Last seen: 5 years, 10 months
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Re: Need help isolating strains [Re: bluemeanie]
#5414379 - 03/17/06 07:03 PM (6 years, 2 months ago) |
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When you say "healthy" do you mean the areas with the most opaque but spotty coverage or the most fluffy but less opaque areas...or areas that are thick, fluffy AND opaque... Well, now that I think of it maybe I'll just isolate one of each and fruit them out.
Still, which areas are considered healthy, the ones with the most coverage?
Thanks for your input.
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The smart way to keep people passive and obedient is to strictly limit the spectrum of acceptable opinion, but allow very lively debate within that spectrum - even encourage the more critical and dissident views. That gives people the sense that there's free thinking going on, while all the time the presuppositions of the system are being reinforced by the limits put on the range of the debate
-Noam Chomsky
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 36,974
Loc: USA Mountain Northwest
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Re: Need help isolating strains [Re: Mobius_Strip]
#5414555 - 03/17/06 08:02 PM (6 years, 2 months ago) |
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Most pan growers select the fuzzy mycelium. Personally, with pans it's easier imo to simply spawn that whole plate into a few grain jars, then later on, clone from your best cluster for a good fruiting strain.
RR
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www.mushroomvideos.com
semper in excretia sumus solim profundum variat
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ohmatic
searcher
Registered: 02/28/04
Posts: 6,276
Loc: europe
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Re: Need help isolating strains [Re: Mobius_Strip]
#5414557 - 03/17/06 08:04 PM (6 years, 2 months ago) |
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all those yellow looking dots on the lower plate do not look promising,
you may be raising some contam in there.
conerning the mycelium, didnt grow pans so far so i cant tell.
peace ohm 
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 MONOTUB tek  HEATBOMB tek
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stay a minimium of 10 minutes after asking a question if you
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Mobius_Strip
Distant Relative
Registered: 03/11/05
Posts: 322
Loc: Spangladesh
Last seen: 5 years, 10 months
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Re: Need help isolating strains [Re: ohmatic]
#5414678 - 03/17/06 09:38 PM (6 years, 2 months ago) |
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Didn't grow pans...huh, are you serious? The print was received from a well known Shroomery vendor and marked as pan goliath. I hope I didn't get stiffed... again.
I received the goliath as a replacement for a dud pan vietnam that I originally purchased and was not only sparse and pencil eraser sized but apparently sterile. I scraped the piss poor pan vietnam paper and tried to make an LC with it. 8 weeks of incubating and nothing happened. Everything else I made into an LC bagan colonizing 3 days after starting.
Those yellow dots aren't really yellow. I was worried about them at first but they haven't proliferated at all. I think they happened as a result of condensation in the dish that dripped down. After replacing the lid with a clean sterile one I noticed them in there. At first glance I thought they might be pins. They're actually clear protrusions kind of like little agar stalagmites or something...They haven't been growing or proliferating but the mycelium doesn't seem to like them either. I don't know really. I figured I'd just keep an eye on them for a while.
Not pans...that's still biting me pretty hard. WTF. I guess I'll just stop using that vendor for good; I don't intentionally give anyone a chance to rip me off a third time. Not cyans...What a pisser!
Edited by Mobius_Strip (03/17/06 09:52 PM)
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ohmatic
searcher
Registered: 02/28/04
Posts: 6,276
Loc: europe
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Re: Need help isolating strains [Re: Mobius_Strip]
#5414711 - 03/17/06 09:51 PM (6 years, 2 months ago) |
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no you got me wrong there !
it looks very much like pan but what i wanted to say was that I havent cultivated paneolus myself yet
and therefor cannot tell you exactly how things are.
sorry for the confusion, im sure the vendor is legit (if one of the boards vendors) 
hope i clarified that
peace ohm
--------------------
MONOTUB tek HEATBOMB tek
#cultivation on irc.shroomery.org for real time cultivation chat
stay a minimium of 10 minutes after asking a question if you
actually expect someone to read and answer it !!!
Edited by ohmatic (03/17/06 09:52 PM)
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Mobius_Strip
Distant Relative
Registered: 03/11/05
Posts: 322
Loc: Spangladesh
Last seen: 5 years, 10 months
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Re: Need help isolating strains [Re: ohmatic]
#5414715 - 03/17/06 09:54 PM (6 years, 2 months ago) |
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Oh. Whew! I was getting mad thinking I got stiffed again. Thanks for clarifying!
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The smart way to keep people passive and obedient is to strictly limit the spectrum of acceptable opinion, but allow very lively debate within that spectrum - even encourage the more critical and dissident views. That gives people the sense that there's free thinking going on, while all the time the presuppositions of the system are being reinforced by the limits put on the range of the debate
-Noam Chomsky
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Workman
Psilocybe Microscopist
Registered: 03/01/01
Posts: 3,094
Loc: Washington, USA
Last seen: 19 hours, 17 minutes
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Re: Need help isolating strains [Re: Mobius_Strip]
#5414720 - 03/17/06 09:57 PM (6 years, 2 months ago) |
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There isn't really a Panaeolus goliath or Panaeolus vietnam, they are both properly named Panaeolus cyanescens and Goliath or Vietnam are only the strain names.
This really doesn't help your problem but I thought it might help clear up some confusion if you happen to acquire a different species of Panaeolus.
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Research funded by the patrons of
The Spore Works
Exotic Spore Supply
Reinvesting 25% of Sales Towards Basic Research and Species Identification  
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Corporal Kielbasa
aka shiek
Registered: 05/29/04
Posts: 14,870
Loc: urmomsroom
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Re: Need help isolating strains [Re: Mobius_Strip]
#5414748 - 03/17/06 10:09 PM (6 years, 2 months ago) |
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With pans i beleave you want light whispy mycelia i could be wrong though.
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Wanted:
Pleurotus eryngii
Pleurotus cystidiosus
Tricholoma conglobatum
Agrocybe aegerita
Flammulina velutipes
Volvariella volvacea
Sparassis crispa
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Mobius_Strip
Distant Relative
Registered: 03/11/05
Posts: 322
Loc: Spangladesh
Last seen: 5 years, 10 months
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Re: Need help isolating strains [Re: Workman]
#5414817 - 03/17/06 10:43 PM (6 years, 2 months ago) |
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Thanks workman,
I always appreciate when others go out of their way to help educate folks.
I was just being lazy really. It's just easier to type "pan goliath" than "Panaeolus cyanescens goliath strain".
I have seen a few reports that the goliath strain is a very poor producer and the vietnamese is a very good producer. Because I'm reletively inexperienced I prefered to have the pan vietnam because of this.
I started the isolations for goliath because I'm wanting to isolate a good producing strain and I wanted to get my feet wet in the world of agar and isolations. I'm having a lot of fun with it so far. I'm particularly proud of my shitake clone on agar, it looks beautiful.
Anyway, I guess if it's really that hard to isolate/distinguish prolific pan strains on agar then I'll just let the dish colonize completely and spawn it to grain, fruit, and clone the best ones. Wish me luck.
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The smart way to keep people passive and obedient is to strictly limit the spectrum of acceptable opinion, but allow very lively debate within that spectrum - even encourage the more critical and dissident views. That gives people the sense that there's free thinking going on, while all the time the presuppositions of the system are being reinforced by the limits put on the range of the debate
-Noam Chomsky
Edited by Mobius_Strip (03/17/06 10:46 PM)
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shroomydan
exshroomerite
Registered: 07/04/04
Posts: 4,126
Loc: In the woods
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Re: Need help isolating strains [Re: Mobius_Strip]
#5415208 - 03/18/06 04:53 AM (6 years, 2 months ago) |
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Quote:
ohmatic said:
didnt grow pans so far so i cant tell.
peace ohm
Ohmatic doesn't mean your culture is not Panaeolus; he means that he has not yet ventured into growing pans, so he can't help you with what the myc should look like.
I think. 
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ohmatic
searcher
Registered: 02/28/04
Posts: 6,276
Loc: europe
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Re: Need help isolating strains [Re: shroomydan]
#5415428 - 03/18/06 08:36 AM (6 years, 2 months ago) |
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Quote:
shroomydan said:
Quote:
ohmatic said:
didnt grow pans so far so i cant tell.
peace ohm
Ohmatic doesn't mean your culture is not Panaeolus; he means that he has not yet ventured into growing pans, so he can't help you with what the myc should look like.
I think.
eeeeeeeeeeeexactamundo
peace ohm
--------------------
MONOTUB tek HEATBOMB tek
#cultivation on irc.shroomery.org for real time cultivation chat
stay a minimium of 10 minutes after asking a question if you
actually expect someone to read and answer it !!!
|
Mobius_Strip
Distant Relative
Registered: 03/11/05
Posts: 322
Loc: Spangladesh
Last seen: 5 years, 10 months
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Re: Need help isolating strains [Re: ohmatic]
#5417311 - 03/18/06 08:30 PM (6 years, 2 months ago) |
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hehehe, yea, I got what ohmatic meant a few posts back  , thanks for the heads up anyway. I can be a bit exciteable at times. 
I'll do what you recommend and just isolate a bunch of different types and fruit them all out. Thanks for the advice.
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The smart way to keep people passive and obedient is to strictly limit the spectrum of acceptable opinion, but allow very lively debate within that spectrum - even encourage the more critical and dissident views. That gives people the sense that there's free thinking going on, while all the time the presuppositions of the system are being reinforced by the limits put on the range of the debate
-Noam Chomsky
Edited by Mobius_Strip (03/18/06 08:31 PM)
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EonTan
bird
Registered: 08/18/04
Posts: 468
Loc: very south
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Re: Need help isolating strains [Re: Mobius_Strip]
#5427043 - 03/21/06 06:35 PM (6 years, 2 months ago) |
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The top half of the top plate looks pretty uniform to me. That is where I would isolate from.
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Mobius_Strip
Distant Relative
Registered: 03/11/05
Posts: 322
Loc: Spangladesh
Last seen: 5 years, 10 months
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Isolations complete. Results pending.
Thanks for your help!
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The smart way to keep people passive and obedient is to strictly limit the spectrum of acceptable opinion, but allow very lively debate within that spectrum - even encourage the more critical and dissident views. That gives people the sense that there's free thinking going on, while all the time the presuppositions of the system are being reinforced by the limits put on the range of the debate
-Noam Chomsky
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