Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!
|
 crakkattak
Would you testthis pill?
 
Registered: 02/19/06
Posts: 471
Last seen: 3 years, 9 months
|
 Heres my personal outline of the PF-Tek method. Any mistakes or pointers you can add is much needed 
#5434924 - 03/23/06 05:19 PM (7 years, 2 months ago) |
|
|
Here?s an outline of my PF TEK method I?m about to approach. If I have any mistakes written down, please point out so I can fix it. Help is much appreciated
I recently purchased a spore syringe (Dutch Mex King to be exact) from ralphstersspores.com, and hope to receive it next week (I sent the mail order 9 days ago).
So here?s my personal outline of what I?m about to do. If I have any incorrect procedures that I?m about to do, please point out so I can learn the easy way, and not waste as much time
I?m first off going to poke 4 holes in my half pint canning jars. The holes are going to be about the size of a ball-point bic pen, because it is slightly bigger then the needle of the spore syringe. After I?ve poked these holes in the jars, I?m going to put a small square cut piece of electrical tape over it.
To mix the substrate together, I?m going to use just a regular mixing bowl from my cabinet. I?m going to use 2 parts vermiculite, 1 part Brown Rice Flour, and 1 part water, then I?m going to mix it together well with a mixing spoon. Once it is all mixed well together, I?m going to fill up my half pint jars till it reaches about 1-2cm from the top. To fill the 1-2cm, I?m going to add dry vermiculite, so it will add a layer to prevent contaminates from reaching the cake, and to give the jars to more breathing area, right?
Once these jars are filled, I?m going to close the lids tightly on the jars. Next, I?m going to clean the inside of my pressure cooker, then stick the jars into it, without cramming it. I set the jars on the rack, and fill the pressure cooker with water. Since I haven?t bought my pressure cooker yet, here?s how much distilled water (estimated) I?ll put into it. If I get a 17-quart pressure cooker, I?ll add about 3 quarts water into it. I just have to add water, but not so much that the jars start to float and tip over (which should be approximately 1inch (2.5cm) of water)
So once I got my water situation figured out, I will close the pressure cooker with the water and jars in it. I will set the pressure cooker to run at 15 psi=1atm for 45mins to an hour. Once it?s done sterilizing, I will wait until the pressure cooker and the jars are cooled off, which should be about 6 hours. Once the jars are cooled off, I will take them out of the pressure cooker, and place in a sterile area. I will then flame the spore syringe needle to eliminate contams, and quickly peel off the tape and inject the needle through the hole into the cake about 2.5cm into the jar, and eject 1-1.5 ml/cc?s into each jar. So about 0.4 ml/cc?s into each hole. Once it has been injected, quickly pull the needle out, fold tape back over the hole, and flame sterilize the needle again and put the cap back on the syringe.
Once the jars have all been inoculated, I will set these jars in my homemade incubator, which looks like this
http://img458.imageshack.us/my.php?image=incubator7iq.png
I will preheat this incubator, and measure the temperature, so it will be around 27 degrees Celsius. Once I have this temperature going, I will set my jars in the upper bucket, and put a towel or blanket over it, so heat cannot escape and the jars will be in a dark area. I will check the temperature and water level every couple days, and if the water level goes down any bit, I will add water to fill back to normal.
After about 2 weeks of the jars being in the incubator, I will check the jars for contams and see how far the mycelium has grown. Now I don?t know whether or not to remove the tape from the jar lids and place a coffee filter over it to let CO2 escape. Is this a good idea? And if I do place a coffee filter on the lid, do I place the jar back in the incubator to fully colonize? Or should I just forget about this coffee filter method and let the jar fully colonize in the incubator, without releasing the CO2 built up in the jar
Once the mycelium has spread to cover all of the substrate and is starting to pin, I will take the jars out of the incubator and place in my fruiting chamber
By doing this I will take the jar, screw off the ring and turn the jar upside down and let it the colonized cake slowly come out of the jar, and into the fruiting chamber, with the cake on top of the lid, which will be on the perlite.
My fruiting chamber isn?t that advanced, but here?s what it looks like, and I think it should work. Its an aquarium by the way
http://img458.imageshack.us/my.php?image=fruitingchamber5oa.png
I made a homemade lid for my aquarium, so it was air tight. The homemade piece you see there is there because I did not want to buy an air pump and I figured this would work. Basically no air contams can fall into the aquarium, but air contams can rise up into it, which I figured would be a low chance of happening. As I believe, once the seal is removed, CO2 should be able to flow through and down this piece, and oxygen should be able to rise up through this piece and into the aquarium.
I will leave my cakes in the fruiting chamber at a temperature of 24.5-26.5 degrees Celsius. I will remove the seal from my homemade air exchange piece for 2-3 hours every day. I will try and keep my humidity around 93% to 99%.
After a week or 2 of my cakes being in the fruiting chamber, they should be ready to harvest. Once harvested, I will try the dunking method for my cakes. I will do this by grabbing the cake with my clean hands and placing it in a sterile container with cold sterile water, being fully submerged. I will leave the container in the fridge for 6-12 hours, then remove the cake from the container place it back in my fruiting chamber, to let another flush hopefully come.
I think this method of mine will work, but I figure you guys will know more than me, and can help point out some pointers I might consider
Thanks
--------------------
   
J-to tha R-O-C Knomsayn!
|
 Citric
Registered: 03/19/04
Posts: 4,073
|
 Re: Heres my personal outline of the PF-Tek method. Any mistakes or pointers you can add is much needed [Re: crakkattak]
#5434964 - 03/23/06 05:36 PM (7 years, 2 months ago) |
|
|
Quote:
Once these jars are filled, I?m going to close the lids tightly on the jars.
Tighten them tight, then loosen them up. When your using tape if you make it tight, once you take the tape off it will be a vacuum inside there. You dont have to use tape. Foil is more then enough.
Quote:
Once it has been injected, quickly pull the needle out, fold tape back over the hole, and flame sterilize the needle again and put the cap back on the syringe.
You only need to flame it once every jar or so. Make sure you whipe with alc after every innoc.
Quote:
Now I don?t know whether or not to remove the tape from the jar lids and place a coffee filter over it to let CO2 escape.
Once the jars germinate, take the tape off. The tape is just to prevent water from entering during PC'ing. You do not need to add a coffee filter or anything. The dry verm is the contam barrier. You can add a filter though, as it sure as hell wouldnt hurt. Mist it down with some alc first.
Quote:
Once the mycelium has spread to cover all of the substrate and is starting to pin, I will take the jars out of the incubator and place in my fruiting chamber
Dont wait for them to pin. Once they are 100% colonized, dunk them, roll them in verm, and double end case them. Dont forget to get rid of that dry verm layer first.
Quote:
which will be on the perlite.
Perlite is so messy, do yourself a favor and get geo-lite/hydroton from a local hydroponic store. Also, check out the poormans pod. Really good terrarium!
Quote:
I will do this by grabbing the cake with my clean hands and placing it in a sterile container with cold sterile water, being fully submerged. I will leave the container in the fridge for 6-12 hours, then remove the cake from the container place it back in my fruiting chamber, to let another flush hopefully come.
I use the same 1/2 pint jars. Use 1/10 h2o2 solution.
Goodluck.
--------------------
Self Healing lid tek
Fast, easy, fool proof inoculation
Mini casing pictures: Pins to harvest
Cup O' Shrooms
Magash: I noticed my contams were in the shape of fingers 
Hyphae: Yes "Loss of moisture from the substrate" is not a casing trigger. 
My final Grow!
|
crakkattak
Would you testthis pill?
Registered: 02/19/06
Posts: 471
Last seen: 3 years, 9 months
|
Re: Heres my personal outline of the PF-Tek method. Any mistakes or pointers you can add is much needed [Re: Citric]
#5435050 - 03/23/06 06:16 PM (7 years, 2 months ago) |
|
|
When you said "Dont wait for them to pin. Once they are 100% colonized, dunk them, roll them in verm, and double end case them. Dont forget to get rid of that dry verm layer first." why would I roll my colonized cake in verm?
And what does double end case them mean?
And I thought your suppose to keep dry verm layer on your cake. How would get rid of the dry verm layer? Just scrape off with something sterile?
--------------------
J-to tha R-O-C Knomsayn!
|
Citric
Registered: 03/19/04
Posts: 4,073
|
Re: Heres my personal outline of the PF-Tek method. Any mistakes or pointers you can add is much needed [Re: crakkattak]
#5435120 - 03/23/06 06:39 PM (7 years, 2 months ago) |
|
|
Just rub it off with a clean glove or something.
Double end case means to put wet verm under, and ontop the cake. Almost acts like a casing.
--------------------
Self Healing lid tek
Fast, easy, fool proof inoculation
Mini casing pictures: Pins to harvest
Cup O' Shrooms
Magash: I noticed my contams were in the shape of fingers
Hyphae: Yes "Loss of moisture from the substrate" is not a casing trigger.
My final Grow!
|
FooMan
Registered: 02/02/05
Posts: 8,279
Loc: Earth 
|
Re: Heres my personal outline of the PF-Tek method. Any mistakes or pointers you can add is much needed [Re: crakkattak]
#5435128 - 03/23/06 06:41 PM (7 years, 2 months ago) |
|
|
Quick question- What's wrong with the current PF tek outlined on this and other websites?
--------------------
Everything you ever wanted to know about growing mushrooms- RIGHT HERE!
New to the Shroomery? GETTING STARTED
If you have time to post a question and wait for an answer, you have time to look up your answer. Lazy questions produce lazy answers. We aren't here to walk you from germination to sporulation. Research, get the basics down, THEN ask questions if you need clarification.
|
Citric
Registered: 03/19/04
Posts: 4,073
|
Re: Heres my personal outline of the PF-Tek method. Any mistakes or pointers you can add is much needed [Re: FooMan]
#5435208 - 03/23/06 07:07 PM (7 years, 2 months ago) |
|
|
Quote:
FooManShroom said:
Quick question- What's wrong with the current PF tek outlined on this and other websites?
Nothing =)
--------------------
Self Healing lid tek
Fast, easy, fool proof inoculation
Mini casing pictures: Pins to harvest
Cup O' Shrooms
Magash: I noticed my contams were in the shape of fingers
Hyphae: Yes "Loss of moisture from the substrate" is not a casing trigger.
My final Grow!
|
MLBjammer
Invitro Freak
Registered: 02/24/06
Posts: 550
Loc: Southeastern USA
Last seen: 4 days, 10 hours
|
Re: Heres my personal outline of the PF-Tek method. Any mistakes or pointers you can add is much needed [Re: FooMan]
#5435231 - 03/23/06 07:14 PM (7 years, 2 months ago) |
|
|
Dude, you need to print out the PF Tek, or the MMGG, or whatever, and follow it exactly. You will make mistakes when you try to innovate without ever having succeeded. If nothing else, read some posts here to familiarize yourself with common terms. Best of luck.
|
FooMan
Registered: 02/02/05
Posts: 8,279
Loc: Earth
|
Re: Heres my personal outline of the PF-Tek method. Any mistakes or pointers you can add is much needed [Re: MLBjammer]
#5435871 - 03/23/06 10:32 PM (7 years, 2 months ago) |
|
|
Quote:
MLBjammer said:
Dude, you need to print out the PF Tek, or the MMGG, or whatever, and follow it exactly. You will make mistakes when you try to innovate without ever having succeeded. If nothing else, read some posts here to familiarize yourself with common terms. Best of luck.
Not sure if you meant to reply to me, but I'll keep that in mind!  
--------------------
Everything you ever wanted to know about growing mushrooms- RIGHT HERE!
New to the Shroomery? GETTING STARTED
If you have time to post a question and wait for an answer, you have time to look up your answer. Lazy questions produce lazy answers. We aren't here to walk you from germination to sporulation. Research, get the basics down, THEN ask questions if you need clarification.
|
krill
absolute gonzo
Registered: 10/25/05
Posts: 521
Loc: mars hotel
Last seen: 10 months, 18 days
|
Re: Heres my personal outline of the PF-Tek method. Any mistakes or pointers you can add is much needed [Re: FooMan]
#5436041 - 03/23/06 11:27 PM (7 years, 2 months ago) |
|
|
i didnt read your post cuz its long and i'm eating dinner and drinking  but my advice to ANYONE making PF / BRF&Verm cakes is this:
ADD FLAXSEED MEAL TO YOUR CAKES!!!!!
i CANNOT stress this ENOUGH!!!
add 1 tsp. of flaxseed meal to each 1/2 pint jar.
this adds a SHITLOAD (seriously) of exxtra nutrients to each substrate and it WILL mos def give you a better output and growing experience. trust me. never fails.
--------------------
"DO NOT ADJUST YOUR MIND. IT IS REALITY THAT IS MALFUNCTIONING." - Robert Anton Wilson
NO LEFT TURN UN-STONED
| |
|
|
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Magash, Az0th, george castanza, Prisoner#1, RogerRabbit, Citric, total, FooMan, stonesun, EvilMushroom666, Javadog
1,306 topic views. 16 members, 74 guests and 0 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic ]
| | |
|
|
|
Images only 
Threaded
Previous
Index
Next
Edit 
Reply 
