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 shroomaker
Stranger
Registered: 04/13/05
Posts: 191
Last seen: 2 years, 7 months
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 Short cut for producing sawdust spawn? 
#5072176 - 12/16/05 06:04 PM (7 years, 6 months ago) |
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I basically want to grow some shiitake, oyster, lion's mane, reishi, and stropharia mushrooms for cultivating outdoor chip beds and also innoculating logs.
Therefore, my question is whether I can use any short cuts to producing sawdust spawn for this purpose. Would it matter if the sawdust spawn got a little contaminated with mold since it would be going outdoors anyhow?
I was wondering how it would work to just take some cleaned shrooms, blend them up and mix them in some sawdust filled bags (or grain spawn) that has been pastuerized. Would this likely produce a semi-successful spawn run? I am trying to avoid the agar -->grain spawn-->sawdust process being I am not setup for this.
If not, I would be curious to learn of any short cut approaches for outdoor purposes.
Thanks!
Edited by shroomaker (12/16/05 06:13 PM)
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 micololo2
Stranger
Registered: 11/05/05
Posts: 388
Loc: Québec, Canada
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Re: Short cut for producing sawdust spawn? [Re: shroomaker]
#5077622 - 12/18/05 11:44 AM (7 years, 5 months ago) |
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>Would it matter if the sawdust spawn got a little contaminated with mold since it would be going outdoors anyhow?
To me a sawdust spawn is use to transfer to a bulk substrat, in that case, yes it would matter. If the question is for a bulk substrat, my answer will be: depend the kind of mush you grow and you'll have to treat it.
>Would this likely produce a semi-successful spawn run? Looking for contam.
>If not, I would be curious to learn of any short cut approaches for outdoor purposes. If u make all the transfers without short cuts I'll give u better chances to get beauties.
For the Lion's Mane I skip the grain transfer. I use liquid spawn that I make, made from agar on plate (I don't use a blender I have another method)direcly on sawdust + 8% wheat bran in 500ml. Mason jars. at 70% full sawdust-wheat bran.
10 cc. liquid spawn on top of sawdust., please no shaking.
Each jar is for inoculate a 5pds. bag for spawn (10% wheat bran)or bulk substrat (20-30% wheat bran).
I would imagine that will be great for stropharia to. I started a patch a few years ago so now I don't have to work inside with them anymore saving time.
You don't say for what kind of oyster.
Reishi, I don't know that culture.
Shiitake, I've never tried to skip.
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mattymonkey
Feel Like aStranger...
Registered: 11/07/04
Posts: 973
Last seen: 5 months, 8 days
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Re: Short cut for producing sawdust spawn? [Re: micololo2]
#5079364 - 12/18/05 08:53 PM (7 years, 5 months ago) |
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interesting post micolo, you skip grain for the lions mane eh? and dont shake the sawdust jars? how long does it take to grow threwout the jar?
--------------------
"listening for the secret.. searching for the sound.."
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micololo2
Stranger
Registered: 11/05/05
Posts: 388
Loc: Québec, Canada
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Re: Short cut for producing sawdust spawn? [Re: mattymonkey]
#5079677 - 12/18/05 10:15 PM (7 years, 5 months ago) |
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Around 2 weeks.
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socialnorm77
forager
Registered: 05/20/05
Posts: 205
Loc: north east
Last seen: 22 days, 16 hours
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Re: Short cut for producing sawdust spawn? [Re: shroomaker]
#5079823 - 12/18/05 10:46 PM (7 years, 5 months ago) |
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Quote:
shroomaker said:
I basically want to grow some shiitake, oyster, lion's mane, reishi, and stropharia mushrooms for cultivating outdoor chip beds and also innoculating logs.
I was wondering how it would work to just take some cleaned shrooms, blend them up and mix them in some sawdust filled bags (or grain spawn) that has been pastuerized. Would this likely produce a semi-successful spawn run?
Your fastest shortcut would be to just order grain spawn. Unless of course you already have specific isolates that you want to use. There are vendors out there that are very reliable and much, much cheaper than other better advertised companies. For example, You can purchase a six pound bag of rye spawn for about $16 US. plus shipping. Not sure where you are located. For this price it will basically pay for itself when you consider the amount of labor to create all that spawn, especially without an adequate lab.
PM me if you would like to know the vendor. (i'd prefer not to solicit)
cheers-norm
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shroomydan
exshroomerite
Registered: 07/04/04
Posts: 4,126
Loc: In the woods
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Re: Short cut for producing sawdust spawn? [Re: shroomaker]
#5095746 - 12/22/05 10:48 PM (7 years, 5 months ago) |
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A while back, I purchased a copy of the h2o2 tech from the mycologist who invented it. He reported successfully creating oyster spawn by by chopping up immature mushrooms and mixing them with h2o2 treated straw.
It might be worth a try.
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shroomaker
Stranger
Registered: 04/13/05
Posts: 191
Last seen: 2 years, 7 months
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Re: Short cut for producing sawdust spawn? [Re: shroomydan]
#5098834 - 12/23/05 07:32 PM (7 years, 5 months ago) |
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I just inititated a couple of experiments today along these lines. One was a immature shiitake I blended and put in H202 treated grain. The other was a shiitake spore mass slurry I treated grain spawn with. I am not real optimistic but it should be fun to see how it turns out. I will report in as to results.
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micololo2
Stranger
Registered: 11/05/05
Posts: 388
Loc: Québec, Canada
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Re: Short cut for producing sawdust spawn? [Re: shroomaker]
#5099385 - 12/23/05 10:10 PM (7 years, 5 months ago) |
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1. Good sucess with H2O2 treated substrat is harder with Shiitake (but not impossible) = To slow growing species. The effect of the H2O2 could be finish before the substrat will be colonize. Make a high rate inoculation. You need a fast growing strain to get better chances.
2. Your young mushroom could inhibate the effect of H2O2.
3. The spores have a good chance to be damage by the H2O2.
4. Starting on treated agar with a healthy mycelium will give u a lot more chances to get what u want.
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shroomaker
Stranger
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Posts: 191
Last seen: 2 years, 7 months
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Re: Short cut for producing sawdust spawn? [Re: micololo2]
#5100982 - 12/24/05 10:03 AM (7 years, 5 months ago) |
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Actually, I didn't use any H202 for the spore mass slurry. I sterilized some grain spawn and used the technique in GMGM to produce the spores. For the small shroom, I just washed it and soaked in some H202 to help sterilize it before blending. The grain spawn was sterilized first by conventional method before adding the blended shroom. It will be interesting to see what happens (if anything).
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