|
 EonTan
bird
Registered: 08/18/04
Posts: 468
Loc: very south
|
 Re: The Return of the Red Spore Cubensis [Re: Hotnuts]
#4566350 - 08/22/05 11:59 AM (3 years, 4 months ago) |
|
|
I am not arguing against the people, I am arguing against the statement made, its wording, and the lack of scientific technology to do what was implied.
Now you can call me negative, and tell me I belong in the Cult forum, not the advanced forum. But you are just showing your lack of knowledge of me, the site, the mushroom itself, and the science surrounding this hobby.
If it is a vegetative hybrid, then yes you could remove the PR nuceli, and replace it with a Redspore nuceli. But if you had multiple compatable nuceli from the redspore you would not need the PR to mate with.
|
OldSpice
Geritol Breath...
Registered: 08/25/03
Posts: 58,389
Loc: Northern Hemisphere..
|
 Re: The Return of the Red Spore Cubensis [Re: Roadkill]
#4566361 - 08/22/05 12:03 PM (3 years, 4 months ago) |
|
|
Im still laughing about Babys 11th level post 
--------------------
So hard to be ....WDWGFH?
Texas is humongus compared to France
Our Gair, who art in Texas,
Paw Paw be thy Name....
My friends are thirsty
Start trotting Eddie
You never see a motorcycle parked outside a Psychiatrist office
|
 RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 19,047
Loc: USA Mountain Northwest
Last seen: 2 hours, 28 minutes
|
Re: The Return of the Red Spore Cubensis [Re: EonTan]
#4566457 - 08/22/05 12:46 PM (3 years, 4 months ago) |
|
|
Quote:
EonTan said:
But if you had multiple compatable nuceli from the redspore you would not need the PR to mate with.
Bingo.
RR
--------------------
www.mushroomvideos.com
Ignorance is contagious. Boycott Walmart and watch your IQ go up.
RR
|
Shdwstr
Aging Reprobate
Registered: 02/17/01
Posts: 2,121
Loc: Ontario, Canada
Last seen: 2 days, 9 hours
|
Re: The Return of the Red Spore Cubensis [Re: EonTan]
#4566768 - 08/22/05 02:42 PM (3 years, 4 months ago) |
|
|
Quote:
EonTan said:
... if you had multiple compatable nuceli from the redspore you would not need the PR to mate with.
That's what my wife keeps telling me... But I tell her we're still getting lucky 
|
Anno
Experimenter
Registered: 06/17/99
Posts: 24,013
Loc: my room
Last seen: 4 days, 14 hours
|
Re: The Return of the Red Spore Cubensis [Re: RogerRabbit]
#4566846 - 08/22/05 03:10 PM (3 years, 4 months ago) |
|
|
Quote:
RogerRabbit said:
Those spores are burgundy. The color of red wine.
Hmmm....but isn't this the color all P. cubensis have? At least the strains I grew do.....
--------------------
The Rich Jerk
Grow Mushrooms from Spores - PF-Tek for Simple Minds
|
CLUTCH
Registered: 04/11/05
Posts: 875
Last seen: 6 days, 38 minutes
|
Re: The Return of the Red Spore Cubensis [Re: RogerRabbit]
#4568236 - 08/22/05 09:02 PM (3 years, 4 months ago) |
|
|
I see red, well actually a maroon.
Cool
|
dr0mni
My Own Messiah
Registered: 08/21/04
Posts: 2,921
Loc: USF Tampa, Fl
Last seen: 1 year, 6 months
|
Re: The Return of the Red Spore Cubensis [Re: CLUTCH]
#4568529 - 08/22/05 09:55 PM (3 years, 4 months ago) |
|
|
yeah, it's really hard to tell. The darkest part of the stipe definitely looks like normal black/purple cube spores, but if you look right below the cap you can catch a hint of the red...
But those pics are of the crossed strains right? So those aren't the final product....
--------------------
 
http://www.myspace.com/sarasotafnb
http://www.wcwtampa.com
|
Innominate
Registered: 06/12/05
Posts: 2,086
|
Re: The Return of the Red Spore Cubensis [Re: dr0mni]
#4571741 - 08/23/05 05:18 PM (3 years, 4 months ago) |
|
|
I think it's a beauty.
|
Dragonaut
SLEEP
Registered: 06/24/04
Posts: 1,812
|
Re: The Return of the Red Spore Cubensis [Re: RogerRabbit]
#4611326 - 09/02/05 01:29 AM (3 years, 4 months ago) |
|
|
any progress? updates?
--------------------
|
blackout
Microwaver
Registered: 07/16/00
Posts: 3,052
Loc: Ireland
Last seen: 5 months, 18 days
|
Re: The Return of the Red Spore Cubensis [Re: Dragonaut]
#4611581 - 09/02/05 03:57 AM (3 years, 4 months ago) |
|
|
Update is that the new issue of the magazine with the prints is now out
EDIT: I was mistaken, the prints are not out yet
--------------------
Visit & Support Free Spore Ring Europe
Microwave Growlog
How to search this site, most of your questions are already answered
Sclerotia, the easiest thing for beginners to grow, high yielding, no fanning/misting/terrarium/light/humidity needed
Edited by blackout (09/02/05 12:12 PM)
|
RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 19,047
Loc: USA Mountain Northwest
Last seen: 2 hours, 28 minutes
|
Re: The Return of the Red Spore Cubensis [Re: blackout]
#4611915 - 09/02/05 10:30 AM (3 years, 4 months ago) |
|
|
Work is ongoing. For those of you who want a redboy/PR cross, I believe free spores will be sent out in a future issue of Teo. Work is underway to duplicate the experiment with the offspring to produce a 75% Redboy/25% PR. If that proves to be successful, a third cross will be made to produce an 87 1/2% Redboy/12 1/2% PR. Such things take time.
RR
--------------------
www.mushroomvideos.com
Ignorance is contagious. Boycott Walmart and watch your IQ go up.
RR
|
MikeOLogical
Doctor ofShroomology
Registered: 01/30/04
Posts: 4,088
Loc: www.pfjars.com
|
Re: The Return of the Red Spore Cubensis [Re: blackout]
#4611959 - 09/02/05 10:43 AM (3 years, 4 months ago) |
|
|
The August issue doesn't have a print of the red spore cube... there was a black and white photocopy of the red spore print, and a real print sample of another species...
was i mistaken? did Peele not announce that there would be a print sample of the red spore in the august issue? i was disappointed when i saw there wasn't...
--------------------
We got Nothing!
we're no longer selling jars. Look for us in December '05, with a new line of tasty gourmet mushroom treats 
|
EonTan
bird
Registered: 08/18/04
Posts: 468
Loc: very south
|
Re: The Return of the Red Spore Cubensis [Re: RogerRabbit]
#4612041 - 09/02/05 11:22 AM (3 years, 4 months ago) |
|
|
IS the methodology of the experiment in the issue?
Repeating the experiment will select towards PR not Redspore.
50:50 hybrid breed to 100% PR will yield 75% PR not Redspore.
75:25 hybrid breed to 100% PR will yield 87.5% PR not redspore.
|
EonTan
bird
Registered: 08/18/04
Posts: 468
Loc: very south
|
Re: The Return of the Red Spore Cubensis [Re: EonTan]
#4612083 - 09/02/05 11:30 AM (3 years, 4 months ago) |
|
|
you have to breed a monokaryon of the hybrid spores back against the protoplasts of the Redspore.
50:50 hybrid mated to 100% redspore yields 75:25 in favor of redspore.
EDIT:
Curious if the trait was passed to all the offspring of the hybrid spores, or just a percentage. How are you going to determine which monokaryon to breed back against the original protoplasts, IE, how do you determine which monokaryon has Redspore trait?
Edited by EonTan (09/02/05 11:42 AM)
|
blackout
Microwaver
Registered: 07/16/00
Posts: 3,052
Loc: Ireland
Last seen: 5 months, 18 days
|
Re: The Return of the Red Spore Cubensis [Re: EonTan]
#4612312 - 09/02/05 12:16 PM (3 years, 4 months ago) |
|
|
Quote:
EonTan said:
IS the methodology of the experiment in the issue?
I don't think so. There are some clues as to how it was done on mycotopia. all zooming over my head, my cats name is mittens
--------------------
Visit & Support Free Spore Ring Europe
Microwave Growlog
How to search this site, most of your questions are already answered
Sclerotia, the easiest thing for beginners to grow, high yielding, no fanning/misting/terrarium/light/humidity needed
|
RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 19,047
Loc: USA Mountain Northwest
Last seen: 2 hours, 28 minutes
|
Re: The Return of the Red Spore Cubensis [Re: blackout]
#4614868 - 09/02/05 11:32 PM (3 years, 4 months ago) |
|
|
quote:
"Curious if the trait was passed to all the offspring of the hybrid spores, or just a percentage"
Just a percentage.
quote:
"How are you going to determine which monokaryon to breed back against the original"
By duplicating the experiment with dozens of monokaryons then seeing which ones work best by the results obtained. In other words, trial and error. Those that fail to pass on the trait are to be discarded.
The methodology is not in the August issue. Look for a paper on it in a future issue, most likely the one with the free spore print.
RR
--------------------
www.mushroomvideos.com
Ignorance is contagious. Boycott Walmart and watch your IQ go up.
RR
|
Workman
Psilocybe Microscopist
Registered: 03/01/01
Posts: 2,438
Loc: Washington, USA
Last seen: 10 hours, 7 minutes
|
Re: The Return of the Red Spore Cubensis [Re: EonTan]
#4617744 - 09/03/05 09:41 PM (3 years, 4 months ago) |
|
|
There is no need to breed the hybrid spores back to the PR. Just do multispore streaks of the hybrid and growout several of the resulting strains. Then it is just a matter of selecting the reddest spores and doing it again until the trait is stable.
--------------------
Research funded by the patrons of
The Spore Works
Exotic Spore Supply
Reinvesting 25% of Sales Towards Basic Research and Species Identification  
|
RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 19,047
Loc: USA Mountain Northwest
Last seen: 2 hours, 28 minutes
|
Re: The Return of the Red Spore Cubensis [Re: Workman]
#4618714 - 09/04/05 04:48 AM (3 years, 4 months ago) |
|
|
Yea, that's being done too. It was probably a typo, but the hybrid spores are being crossbred back to the original Redboy, not the PR. It would be nice to attempt to stabilize the red spore trait from a 75% Redboy, rather than a 50/50.
RR
--------------------
www.mushroomvideos.com
Ignorance is contagious. Boycott Walmart and watch your IQ go up.
RR
|
EonTan
bird
Registered: 08/18/04
Posts: 468
Loc: very south
|
Re: The Return of the Red Spore Cubensis [Re: Workman]
#4622245 - 09/05/05 11:46 AM (3 years, 4 months ago) |
|
|
The original Redspore spores could have been treated, and possibly had dikaryons emerge without hybridization.
Since the hybrid monokaryons must be mated against those original treated spores to get 75% Redspore, why not just mate the Redspores amongst themselves. To have any mating, they must have been succesfully removed from the cell walls, and could have undegone fusion with each other.
Yes the hybrid spores them selves can be used to isolate strains that contain the redspore trait, without having to backcross. You can never get back to 100% redspore anyways.
There really was no need to hybridize in the first place, and there is no need to backcross now. Just isolate strains that have the redspore trait, via multispore, or via controlled monokaryon matings to know for sure which monokaryons posses the trait.
Backcrossing is not going to gurantee redspore trait at 75 %, just that 75% of the genome is Redspore strain.
The only gurantee is controlled matings of hybrid monokaryons to determine which monokaryons exactly posses the trait, and which combinations when mated posses the trait, and which of these pass it to 100% of the offspring.
This can be done random as workman suggest, easiest method. Or controlled, to gurantee results, not so easy.
|
RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 19,047
Loc: USA Mountain Northwest
Last seen: 2 hours, 28 minutes
|
Re: The Return of the Red Spore Cubensis [Re: EonTan]
#4622480 - 09/05/05 12:48 PM (3 years, 4 months ago) |
|
|
quote:
"There really was no need to hybridize in the first place, and there is no need to backcross now."
It is indeed an honor to have someone of your caliber and expertise to point out everything I've done wrong. By the way, what IS your caliber and experience in getting 20 year old spores to germinate? The 20 year old redboy print I received would NOT germinate, no matter what I tried, and believe me, I nearly ran out of spores trying every trick in the book, plus a few not in the book. In my experience, after even just five years, a room temperature stored sporeprint taken on non-acid free paper will not germinate. In fact, it was just such a five year old sporeprint that was used to develop the procedures listed in this thread. Once the experiment had been duplicated with the five year old sporeprint, the tek was applied to the 20 year old spores which would NOT germinate on their own. Hybridization was mandatory because there were no fresh redboy spores in existence anywhere.
RR
--------------------
www.mushroomvideos.com
Ignorance is contagious. Boycott Walmart and watch your IQ go up.
RR
|
|
login or 
register to post messages and view our members-only content. You'll gain access to additional forums, encrypted messages, file attachments, board customizations, and much more!
Previous
Index
Next
Threaded
Edit 
Reply 
Quote 
