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 Workman
Psilocybe Microscopist
Registered: 03/01/01
Posts: 2,438
Loc: Washington, USA
Last seen: 8 hours, 29 minutes
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 Results of multispore inoculation, Psilocybe mexicana, Jalisco
#3774672 - 02/13/05 03:30 PM (3 years, 10 months ago) |
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Small multispore test tray of Psilocybe mexicana, Jalisco strain. Grass seed cased with peat/calcium carbonate.
Selective harvest of various phenotypes of the above tray. The one on the far right was deemed the best and has been cloned for future work.
 
Clone isolated and fruited - 1st flush. Note the quality of the mushroom shape, color and spore production.
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Reinvesting 25% of Sales Towards Basic Research and Species Identification  
Edited by Workman (05/09/05 01:33 PM)
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Teo Torriatte
Registered: 03/03/04
Posts: 129
Loc: Over at the Frankenstein ...
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Re: Results of multispore inoculation, Psilocybe mexicana, Jalisco [Re: Workman]
#3774695 - 02/13/05 03:36 PM (3 years, 10 months ago) |
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now that's how it's done. those actually look like mexicana!
good to see you posting so much lately
Biff
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tonight you'll fall asleep in clothes-so late like a candy bar wrapped up for lunch that's all you get to taste
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poverty and spit
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Psilygirl
cyan goddess
Registered: 08/28/03
Posts: 4,417
Loc: PNW
Last seen: 22 days, 32 minutes
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Re: Results of multispore inoculation, Psilocybe mexicana, Jalisco [Re: Workman]
#3774782 - 02/13/05 04:03 PM (3 years, 10 months ago) |
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once again I am very impressed. keep up the good work, they look beautiful 
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"Love says 'I am everything.' Wisdom says 'I am nothing.' Between the two, my life flows."
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Semilanceata
No god, no boss
Registered: 05/26/03
Posts: 841
Loc: República Federal Íbera
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Re: Results of multispore inoculation, Psilocybe mexicana, Jalisco [Re: Workman]
#3777182 - 02/14/05 02:05 AM (3 years, 10 months ago) |
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Very nice!!!
Hey man, I have some questions  (if you wan?t to answer them)
What is your grain/water ratio?
How long did you incubate the grain?
Did you cold shock it for a long time?
At what temps are you fruiting them?
Do you think it?s better to start from multispore instead of agar wedges?
Thanks and GL.
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Sr_Setahongo
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Workman
Psilocybe Microscopist
Registered: 03/01/01
Posts: 2,438
Loc: Washington, USA
Last seen: 8 hours, 29 minutes
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Re: Results of multispore inoculation, Psilocybe mexicana, Jalisco [Re: Semilanceata]
#3779173 - 02/14/05 03:33 PM (3 years, 10 months ago) |
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Grain/water ratio 2:1
Incubated 2 weeks at 75-80F
No cold shock
Fruiting at 75-78F
These were inoculated with agar wedges, but no isolation of strains on the agar was attempted. I personally never use spore syringes.
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Research funded by the patrons of
The Spore Works
Exotic Spore Supply
Reinvesting 25% of Sales Towards Basic Research and Species Identification
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Enter
The Book of Entheogenesis
Registered: 07/27/01
Posts: 6,625
Loc: The Daturas
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 Re: Results of multispore inoculation, Psilocybe mexicana, Jalisco [Re: Workman]
#3780317 - 02/14/05 07:31 PM (3 years, 10 months ago) |
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Beautiful!
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ShroomeroUno
Quick Learner
Registered: 02/09/05
Posts: 204
Loc: FunkyTown,Tx.
Last seen: 3 years, 10 months
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Re: Results of multispore inoculation, Psilocybe mexicana, Jalisco [Re: Enter]
#3780539 - 02/14/05 08:36 PM (3 years, 10 months ago) |
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Awsome.Great Work.
ShroomeroUno
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 Blue Helix
old hand
Registered: 02/02/03
Posts: 974
Last seen: 5 seconds
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Re: Results of multispore inoculation, Psilocybe mexicana, Jalisco [Re: ShroomeroUno]
#3786398 - 02/15/05 10:01 PM (3 years, 10 months ago) |
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Great work, Workman.
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MrMaddHatter
Dementia praecox
Registered: 06/07/02
Posts: 1,409
Loc: Nowhere
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Re: Results of multispore inoculation, Psilocybe mexicana, Jalisco [Re: Workman]
#3787689 - 02/16/05 01:26 AM (3 years, 10 months ago) |
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Two thumbs up!
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ralphster44
Vendor/collector
Registered: 01/03/01
Posts: 4,254
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Re: Results of multispore inoculation, Psilocybe mexicana, Jalisco [Re: Workman]
#3789349 - 02/16/05 01:17 PM (3 years, 10 months ago) |
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WOW.....That is the BEST I have ever seen!!!
My god....that is some great work there!!!
Absoutely wonderful!!!!
Perfect job Workman!!!
Excellent!!!
Holy shit!!!  
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WE SHIP TO CANADA FROM WITHIN CANADA 
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Cyber
Ash
Registered: 06/14/04
Posts: 1,041
Loc: Dearborn Michigan
Last seen: 2 months, 16 days
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Re: Results of multispore inoculation, Psilocybe mexicana, Jalisco [Re: Workman]
#3789393 - 02/16/05 01:27 PM (3 years, 10 months ago) |
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I thought you were supposed to wait 3 months before casing Ps. Mexicana.
Only 2 weeks?
Mexicana is a neat mushroom!
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Shop SMART, Shop S-MART!
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tahoe
Noob Slayer
Registered: 11/26/03
Posts: 4,622
Loc: N38.93829W119.98108
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Re: Results of multispore inoculation, Psilocybe mexicana, Jalisco [Re: ralphster44]
#3789747 - 02/16/05 03:02 PM (3 years, 10 months ago) |
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Quote:
ralphster44 said:
WOW.....That is the BEST I have ever seen!!!
My god....that is some great work there!!!
Absoutely wonderful!!!!
Perfect job Workman!!!
Excellent!!!
Holy shit!!!
he must not have used a bag
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Alder is not the BEST type of wood to use
Heat drying cubes is okay and recommended
Sunflower seeds do not cause contams
You should directly mist your cakes
The subjects that I cover are not species specific and can be used in All Aspects of Mycology
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Semilanceata
No god, no boss
Registered: 05/26/03
Posts: 841
Loc: República Federal Íbera
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Re: Results of multispore inoculation, Psilocybe mexicana, Jalisco [Re: Workman]
#3789812 - 02/16/05 03:20 PM (3 years, 10 months ago) |
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Quote:
Workman said:
These were inoculated with agar wedges, but no isolation of strains on the agar was attempted. I personally never use spore syringes.
So, with psilo mex and maybe other exotic shrooms it?s better to inoc. grain with lots of strains instead of "trying" to isolate a single fruiting strain on agar, right?
You know, I started not long ago with ps.mex and pan cyans cultivation via isolation of strains on agar and the failure is 100%. Everything is healthy but not a single fruit.
Thanks.
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Sr_Setahongo
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Workman
Psilocybe Microscopist
Registered: 03/01/01
Posts: 2,438
Loc: Washington, USA
Last seen: 8 hours, 29 minutes
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Re: Results of multispore inoculation, Psilocybe mexicana, Jalisco [Re: Semilanceata]
#3798652 - 02/18/05 01:37 PM (3 years, 10 months ago) |
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This is just what prefer to do when I only have spores, maybe its not ideal but it works for me. If a fresh mushroom is available I prefer a clone for the first attempt.
--------------------
Research funded by the patrons of
The Spore Works
Exotic Spore Supply
Reinvesting 25% of Sales Towards Basic Research and Species Identification
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pelon
Registered: 12/04/04
Posts: 31
Loc: omnipresent
Last seen: 3 years, 6 days
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Re: Results of multispore inoculation, Psilocybe mexicana, Jalisco [Re: Workman]
#3942246 - 03/19/05 11:20 PM (3 years, 9 months ago) |
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Would you share your agar recipe for use with mexicana? Also how long after a multispore inoculation of the agar do you see growth? Any help would be greatly appreciated.
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 19,047
Loc: USA Mountain Northwest
Last seen: 50 minutes, 53 seconds
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Re: Results of multispore inoculation, Psilocybe mexicana, Jalisco [Re: pelon]
#3942336 - 03/19/05 11:54 PM (3 years, 9 months ago) |
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Very impressive as usual Workman.
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Ignorance is contagious. Boycott Walmart and watch your IQ go up.
RR
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Workman
Psilocybe Microscopist
Registered: 03/01/01
Posts: 2,438
Loc: Washington, USA
Last seen: 8 hours, 29 minutes
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Re: Results of multispore inoculation, Psilocybe mexicana, Jalisco [Re: pelon]
#3943785 - 03/20/05 11:39 AM (3 years, 9 months ago) |
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I just use commercial malt agar. I think its 2% malt.
I generally see growth in 4-10 days at 85F. Depends on how good your eyes are.
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Research funded by the patrons of
The Spore Works
Exotic Spore Supply
Reinvesting 25% of Sales Towards Basic Research and Species Identification
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Olgualion
Shaman-In-Training
Registered: 11/13/01
Posts: 1,197
Loc: Currently Earth...
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Re: Results of multispore inoculation, Psilocybe mexicana, Jalisco [Re: Workman]
#3944000 - 03/20/05 12:39 PM (3 years, 9 months ago) |
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Impressive indeed!!
Always nice to see your work!
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Study the past...
See the future...
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