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 JAMIEK
member
Registered: 07/14/04
Posts: 174
Last seen: 2 years, 8 months
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Scat I will try your way because I have 10 of them
Shmoppy McGillicuddy
And you are also correct in your theory!
I have the chips ready and will pc in the morn and we will go from there I will start a grow log with this "supposed" azurscen strain ....which was a ebay buy so Is this azurscens I guess we will find out together as a whole huh! 
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JAMIEK
member
Registered: 07/14/04
Posts: 174
Last seen: 2 years, 8 months
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 Re: Azurescens / wood chip tek  [Re: JAMIEK]
#3224293 - 10/06/04 06:03 PM (7 years, 7 months ago) |
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OK here are the jars in the wood cased pyrex dishes with visible and abundant groth in about a day in a half! POST MORE WHEN MORE HAPPENS!
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Gr0wer
cultivator &mycologist
Registered: 09/16/03
Posts: 2,423
Last seen: 5 years, 1 month
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 Re: Azurescens / wood chip tek [Re: JAMIEK]
#3224807 - 10/06/04 08:12 PM (7 years, 7 months ago) |
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You should have broken the cakes into pea sized pieces. Also using smaller woodchips or coarse sawdust works better because there's less space between em. Besides that ther looing good!
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JAMIEK
member
Registered: 07/14/04
Posts: 174
Last seen: 2 years, 8 months
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Re: Azurescens / wood chip tek [Re: Gr0wer]
#3229537 - 10/07/04 05:19 PM (7 years, 7 months ago) |
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Thank you for the compliment that makes me feel as if I'm contributing to the forum.I appreciate it!
I couldnt find smaller ones I guess that Ill just chopp em up or something!
I didi find a sawmill that will sell me straight saw dust from the mill like cheap said thatI'd have to take a 50lb bag or so to make it worth it!
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Ekstaza
stranger thanmost
Registered: 04/10/03
Posts: 4,315
Loc: Around the corner
Last seen: 2 months, 29 days
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Re: Azurescens / wood chip tek [Re: JAMIEK]
#3230083 - 10/07/04 07:18 PM (7 years, 7 months ago) |
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You'll still need wood chips as azures are not known to fruit very well on sawdust alone.
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YOUR EXPERIENCE WITH ANY GIVEN DRUG ISN'T THE DEFINITIVE MEASURE OF THE DRUGS EFFECTS.
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jeff01
cultivator
Registered: 12/17/04
Posts: 44
Last seen: 7 years, 4 months
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Re: Azurescens / wood chip tek [Re: Ekstaza]
#3563689 - 12/30/04 10:26 PM (7 years, 4 months ago) |
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looking good, good luck on the az's
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"impossible to walk in this muck"
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scatmanrav
Brainy Smurf
Registered: 05/08/04
Posts: 11,351
Loc: 
Last seen: 18 days, 10 hours
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Re: Azurescens / wood chip tek [Re: jeff01]
#3580210 - 01/04/05 02:47 PM (7 years, 4 months ago) |
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Any updates, Jamiak still around? Just wondering..
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Fluxburn
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Registered: 10/22/04
Posts: 2,216
Loc: Oakland, CA, USA
Last seen: 1 year, 6 months
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Re: Azurescens / wood chip tek [Re: scatmanrav]
#3589339 - 01/06/05 01:56 PM (7 years, 4 months ago) |
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sorry what is brf, I am assuming it's a method to substrate and colonization
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ABSTRACT ART (Mine) http://nathanbelomy.com
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shirley knott
not my real name
Registered: 11/11/02
Posts: 9,086
Loc: London
Last seen: 1 year, 7 months
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Re: Azurescens / wood chip tek [Re: Fluxburn]
#3589345 - 01/06/05 02:00 PM (7 years, 4 months ago) |
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brf = brown rice flour
an ingredient of the 'pf tek'
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meh
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Fluxburn
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Registered: 10/22/04
Posts: 2,216
Loc: Oakland, CA, USA
Last seen: 1 year, 6 months
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Re: Azurescens / wood chip tek [Re: JAMIEK]
#3589485 - 01/06/05 02:45 PM (7 years, 4 months ago) |
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oh duh, I knew the method just the acronym messed me up.
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ABSTRACT ART (Mine) http://nathanbelomy.com
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,064
Loc: my room
Last seen: 1 day, 16 hours
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Re: Azurescens / wood chip tek [Re: Fluxburn]
#3589783 - 01/06/05 03:46 PM (7 years, 4 months ago) |
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Fluxburn
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Registered: 10/22/04
Posts: 2,216
Loc: Oakland, CA, USA
Last seen: 1 year, 6 months
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Re: Azurescens / wood chip tek [Re: JAMIEK]
#3589798 - 01/06/05 03:49 PM (7 years, 4 months ago) |
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lol i forgot it was brown rice flour and went to the store and got wheat flour lol
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ABSTRACT ART (Mine) http://nathanbelomy.com
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Openminded
Dicotyledon
Registered: 08/28/03
Posts: 657
Loc: England.
Last seen: 4 years, 1 month
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Re: Azurescens / wood chip tek [Re: Fluxburn]
#3593226 - 01/07/05 09:34 AM (7 years, 4 months ago) |
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I was wondering if anyone had tried this, kinda a way to go directly from spores to wood spawn in one step in one jar. If you put your soaked chips in a jar, and put a thin layer of PF-tek mixture (brf and vermiculite) on top of that, and then sterilised and inoculated as you would a normal jar, then the spores would germinate and grow onto the chips after the myc had become established on the brf. Seems to me this would save quite a lot of effort, because you wouldn't have to start off with making an agar wedge and then transfering that to spawn under sterile conditions..?
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,064
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Re: Azurescens / wood chip tek [Re: Openminded]
#3593238 - 01/07/05 09:39 AM (7 years, 4 months ago) |
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This should work, providing you have a clean spore syringe.
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Fluxburn
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Registered: 10/22/04
Posts: 2,216
Loc: Oakland, CA, USA
Last seen: 1 year, 6 months
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Re: Azurescens / wood chip tek [Re: JAMIEK]
#3594886 - 01/07/05 04:32 PM (7 years, 4 months ago) |
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Well, honestly I don't think the transfer to wood is such a big deal. As well, you don't need to go from agar to substrate, to casing (hardwood). People to a direct inoculation from syringe to substrate and then go to casing. I am sure what you are suggesting is possible, but I wouldn't attempt for the following reason, infection. If you have the jars or bags in the first stage then you can just isolate the bad ones and get rid of them. While the same is true with the method you describe above, a single substrate and case layer so to speak, seems to myself to be a pure waste of casing materials. The reason for my viewpoint is because once the substrate is fully colonized, Azurescens has what is seems to be a zero percent contamination rate to the casing layer, meaning the wood chips Cannon be infected as easily from natural pathogens, such as bacteria. So basically what I am saying, is your could have one jar of fully colonized Azurescens mycelium and spread it across ten casing of hard wood chips and at that point zero would become infected. The method you describe just would waste hard wood, and would seem to have no real point for time saving. As well, I believe that spawn materials colonize faster then hard wood, thus defeating the purpose of your method entirely. Another reason it would not work as well, is at the casing layer objective point, you would assume gas exchange would become an interesting prospect. Azurescens normally grows outdoors, I am under the assumption that plenty of gas exchange is readily available in the atmosphere, thus getting the colonized materials out of the jars or bags to a gas exchange situation would seem very important. The one benefit I could see to your method is perhaps the wood might colonize faster, thus speeding the entire process, even though this would seem impossible since Azurescens seems to be a slower growing mushroom or perhaps that is my viewpoint from seeing all the pictures of mushrooms very small in nature to other mushrooms of this nature.
And personally for myself, I enjoy more steps in the process since that makes it more complex, thus more interesting. I am actually going to start using agar first just to increase the complexity of my mushroom cultivation. If you think about it actually, you would have to think that since agar grows so rapidly, that it would actually be the fastest way to produce mushrooms and in this case, I believe you could actually just go agar to wood chips and bypass the entire brf, birdseed, rye, etc. since Azurescens grows directly on the wood chips and not the brf, rye, birdseed, cord, etc. as other species do. I am assuming the agar plate has become fully colonized, yet at the same time, even if the agar plate was slightly infected you might be able to get away with it since it might not grow successfully on the hardwood. If I remember correctly, I believe most woods have a natural anti bacteria properties in the sap that is secreted throughout the entire tree. Only a few special forms of tree symbiont's are found in nature, I believe fungi is the only compatible life form available to trees as symbiont's(meaning they do not harm the other object. The conclusion I have found; if you could handle a glove box and making agar plates, I believe that would actually be the fastest and simplest way to make Azurescens by direct transfer to the hardwood.
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ABSTRACT ART (Mine) http://nathanbelomy.com
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,064
Loc: my room
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Re: Azurescens / wood chip tek [Re: Fluxburn]
#3594927 - 01/07/05 04:43 PM (7 years, 4 months ago) |
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I assumed that the jar would be shaken after the brf substrate would have colonized.
As for the contamination, green mold thrives on wood. Don't assume just because you are growing azurescens and using wood that you can forget all the sterile procedures. Unfortunately this is not the case.
Also, a direct transfer from agar to wood colonizes very, very slow. Colonized grain(or brf substrate as in the above suggestion) trasfered to wood guarantees the fastest colonization.
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Fluxburn
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Registered: 10/22/04
Posts: 2,216
Loc: Oakland, CA, USA
Last seen: 1 year, 6 months
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Re: Azurescens / wood chip tek [Re: JAMIEK]
#3595318 - 01/07/05 06:16 PM (7 years, 4 months ago) |
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Thanks for that information on agar to wood transfer. I am just recalling on many individuals success with no sterilization of wood chips for cultivation of Azurescens. Personally I will sterilize the woodchips, since I sterilize everything.
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ABSTRACT ART (Mine) http://nathanbelomy.com
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