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 sonichell
journeyman
Registered: 10/31/99
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 Re: this is how we made our azure spawn (pictorial) [Re: dioze1]
#329931 - 05/30/01 04:47 PM (10 years, 11 months ago) |
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a friend has noticed a prob he had with yellow and black mold hitting jars. BUt he thinks it might have been some woodchips he missed that were in the birdseed, anyone else have this prob, either that or airborne or on a scalpel. THis has hit a quite a few jars from the ass end of a bag of penningtons. ANy ideas? anyone have contam from old lids? I soaked the jars in bleach water even.
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ALDER_057
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Re: this is how we made our azure spawn (pictorial) [Re: Una]
#329987 - 05/30/01 05:42 PM (10 years, 11 months ago) |
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Una, nice post, we need to see more posts of this nature. Thanks for the step by step. peace
ALDER
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Datrutankdogg69
Cyan Man
Registered: 01/11/01
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Loc: Pacific NW, Or.
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Re: this is how we made our azure spawn (pictorial) [Re: Una]
#330486 - 05/31/01 06:57 AM (10 years, 11 months ago) |
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Well Done,
Can?t wait 2 see patch pix.
Later,
#P#
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 Una
controlleddemolition
Registered: 03/01/01
Posts: 970
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Re: this is how we made our azure spawn (pictorial) [Re: Datrutankdogg69]
#330640 - 05/31/01 10:45 AM (10 years, 11 months ago) |
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holographic mind
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Re: this is how we made our azure spawn (pictorial) [Re: dioze1]
#331365 - 06/01/01 04:45 AM (10 years, 11 months ago) |
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hahaha. "my mother is a retired microbiologist and swears 45 minutes are 15 psi will sterilize anything." you are funny. you need to read stamets "growing gourmet and medicinal mushrooms".
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dioze1
Satans minion inthe war onlaughter
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Loc: Up a hogs ass looking for...
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Re: this is how we made our azure spawn (pictorial) [Re: holographic mind]
#331469 - 06/01/01 08:40 AM (10 years, 11 months ago) |
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Call Gerber baby foods and ask who their head Microbacteriologist was for 20 years, I' ll bet yoo 100 dollars the initials will be P.B.
Dioze1
P.S.
Stamets is a pioneer and has contributed greatly to the Mycology community-- But he doesn't know everything and people have been growing mushrooms with out his help or methods for years.
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holographic mind
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Re: this is how we made our azure spawn (pictorial) [Re: dioze1]
#331471 - 06/01/01 08:43 AM (10 years, 11 months ago) |
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"P.S.
Stamets is a pioneer and has contributed greatly to the Mycology community-- But he doesn't know everything and people have been growing mushrooms with out his help or methods for years."
translation = "I haven't read growing gourmet & medicinal mushrooms. i do not realize that holographic mind is refering to comparative sterilization times"
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Una
controlleddemolition
Registered: 03/01/01
Posts: 970
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Re: this is how we made our azure spawn (pictorial) [Re: holographic mind]
#331478 - 06/01/01 08:54 AM (10 years, 11 months ago) |
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ok...so my thread is turning into a "how long should you sterilise something before it's sterile"...
In think(and i do also know about microbiology and plant tissue culture) the required time depends on:
a) if it are liquids or solids
b) the amount of substrate in a container
Liquids require only brief sterilisation, solids require longer (beacuse liquids conduct heat much better, and don't have small cavities)
The more substrate is in a container, the longer it will take for the heat to penetrate to the core..
I believe that exposure to 121oC for 45 minutes will indeed sterilise anything.....but before the core of the substrate has reached that temperature may take quite some time.
I think you are both right...but you both mean a different thing....
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dioze1
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Re: this is how we made our azure spawn (pictorial) [Re: Una]
#331699 - 06/01/01 01:48 PM (10 years, 11 months ago) |
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VERY well said, Una. 45 min@ 15 psi WILL sterilize anything---BUT it all depends on density and mass---to achieve temps at the core of whatever is being sterilized times NEED to be adjusted.
Una, This is an excellent thread!! I commend you for your efforts to educate the masses and your efforts to save YOUR thread.
Dioze1
check out the Free Spore Ring!
http://go.to/FreeSporeRing
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BrownPastures
old hand
Registered: 03/15/01
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Re: this is how we made our azure spawn (pictorial) [Re: Una]
#333447 - 06/03/01 07:14 PM (10 years, 11 months ago) |
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i dont know what all the huffin' and puffin' is about... it still looks like a hole with a bunch of shit in it.... where's the rest?
"like a Japanese Cowboy or a blind man on skates"Ween
"you must make sure that the lady's pure for the Funky Cold Medina"Tone Loc
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slither
addict
Registered: 02/02/01
Posts: 365
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Re: this is how we made our azure spawn (pictorial) [Re: BrownPastures]
#333479 - 06/03/01 08:06 PM (10 years, 11 months ago) |
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I think it's one of the best threads that's been on this board in awhile, sure answered alot of questions for me, went in my archives, thanks!!
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zetek
enthusiast
Registered: 04/28/01
Posts: 210
Loc: United States
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Re: this is how we made our azure spawn (pictorial) [Re: Una]
#333494 - 06/03/01 08:26 PM (10 years, 11 months ago) |
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Very nice Una! I learned a lot from your thread. Thanks!
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John
ssdp.org
Registered: 08/08/03
Posts: 7,026
Loc: Vancouver, B.C.
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Re: this is how we made our azure spawn (pictorial) [Re: Una]
#333870 - 06/04/01 06:58 AM (10 years, 11 months ago) |
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Hi,
Don't you think adding oat is really necessary? I think one can get contams if does not work in really sterile environment. You shouldn't put your culture outdoor until spawn is fully colonized also... When you use pure woodchips, you don't have to keep it home and wait till all is colonized...
Edited by john on 06/04/01 10:01 AM.
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Una
controlleddemolition
Registered: 03/01/01
Posts: 970
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Re: this is how we made our azure spawn (pictorial) [Re: ]
#333885 - 06/04/01 07:24 AM (10 years, 11 months ago) |
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Don't you think adding oat is really necessary?
No but it speeds up colonisation dramatically. It also produces thicker and more luxurious mycelium. Instead of oatmeal you can also use bran.
I think one can get contams if does not work in really sterile environment.
True, but since we have a laminar flow cabinet (really sterile environment) this is no problem.
You shouldn't put your culture outdoor until spawn is fully colonized also...
No you shouldn't...Of course we let the spawn fully colonise before using it outdoors. Indeed if the woodchips are not supplemented putting them outdoors before 100% colonisation should pose less problems..
Need spores? We got spores!
www.mushmush.nl
for the highest quality exotic mushroom spores.
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cornbread
member
Registered: 12/09/00
Posts: 50
Last seen: 10 years, 10 months
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Re: this is how we made our azure spawn (pictorial) [Re: Una]
#334471 - 06/04/01 07:18 PM (10 years, 11 months ago) |
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Nice Job Una,
I was curious did you use your wood spawn for a substrate or did you use it to spawn more wood? How deep is your substrate layer?
Thanks
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Una
controlleddemolition
Registered: 03/01/01
Posts: 970
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Re: this is how we made our azure spawn (pictorial) [Re: cornbread]
#335033 - 06/05/01 08:10 AM (10 years, 11 months ago) |
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The spawn was used to spawn more small wood chips. These small chips were soaked for 24 hours. Also bigger pieces of wood (10 x 2 cm) were thrown in (these were soaked for some days).
I estimate that the bed is 25 cm deep.
Need spores? We got spores!
www.mushmush.nl
for the highest quality exotic mushroom spores.
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cornbread
member
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Re: this is how we made our azure spawn (pictorial) [Re: Una]
#335183 - 06/05/01 12:05 PM (10 years, 11 months ago) |
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Thanks alot,
I enjoyed your post.
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Glacius
Lang
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Re: this is how we made our azure spawn (pictorial) [Re: Suntzu]
#335393 - 06/05/01 04:23 PM (10 years, 11 months ago) |
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Right on. That was extremely interesting. I am going to have to try it. That was an awsome post. I learned quite a bit from that. Thank's.
Glacius
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DarK_SavioR
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Re: this is how we made our azure spawn (pictorial) [Re: Una]
#335604 - 06/05/01 08:11 PM (10 years, 11 months ago) |
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Awesome, I might try growing some flying saucers sometime soon... you should put this info togethor in a nice little tek guide or something if you haven't already... I'm saving this to my shrooming archive though ; )
"Now chew em up and slam the orange juice. Vitamin C chase, kill the taste. You can tell its nasty by the look on my face."
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Una
controlleddemolition
Registered: 03/01/01
Posts: 970
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Re: this is how we made our azure spawn (pictorial) [Re: DarK_SavioR]
#336336 - 06/06/01 03:24 PM (10 years, 11 months ago) |
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We added the above information to our website. It can be found on www.mushmush.nl under the "methods" section.
Una
Need spores? We got spores!
www.mushmush.nl
for the highest quality exotic mushroom spores.
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