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 YSr
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Registered: 04/13/04
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 Oatmeal agar - 2 questions
#3300363 - 10/31/04 04:32 AM (7 years, 3 months ago) |
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80g Oatmeal
20g Agar
10g Dextrose
1000 ml Water
1) Is it best with or without the dextrose?
2) Is it better if adding 2g yeast and/or peptone?
YSr
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 noxy
Dr
Registered: 09/20/04
Posts: 181
Loc: its more a time, not a pl...
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Re: Oatmeal agar - 2 questions [Re: YSr]
#3300879 - 10/31/04 11:17 AM (7 years, 3 months ago) |
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I assume your making a broth with that oatmeal?
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YSr
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Re: Oatmeal agar - 2 questions [Re: noxy]
#3300886 - 10/31/04 11:21 AM (7 years, 3 months ago) |
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I'm using it to make agar..... (?)
YSr
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noxy
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Re: Oatmeal agar - 2 questions [Re: YSr]
#3300897 - 10/31/04 11:27 AM (7 years, 3 months ago) |
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ive never tried an oatmeal agar
a potatoe dextrose agar is easy to make
filter the broth from boiling 300grams of sliced potatoes in i litter of water for an hour
add
10 grams dextrose sugar
2 grams yeast
20 grams agar
water to 1000 ml
I think you would want to avoid having solids floating around in the agar
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YSr
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Re: Oatmeal agar - 2 questions [Re: noxy]
#3300907 - 10/31/04 11:29 AM (7 years, 3 months ago) |
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I know PDYA, I just wondered if oatmeal agar is best with or without dextrose, and
if it is good to add yeast and/or peptone. Anyone else?
YSr
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noxy
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Re: Oatmeal agar - 2 questions [Re: YSr]
#3301514 - 10/31/04 02:45 PM (7 years, 3 months ago) |
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OK what kind of oatmeal are you using?
Ive never known any recipies for oatmeal/oatmeal extract agar to use dextrose
anyone else??
Edited by noxy (10/31/04 02:51 PM)
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ATWAR
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Registered: 01/26/03
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Loc: #108768 in line...
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Re: Oatmeal agar - 2 questions [Re: YSr]
#3303282 - 11/01/04 12:08 AM (7 years, 3 months ago) |
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Quote:
YSr said:
I know PDYA, I just wondered if oatmeal agar is best with or without dextrose, and
if it is good to add yeast and/or peptone. Anyone else?
YSr
You could add dextrose to the brew if you like, but I wouldn't push it. Adding too much sugars will cause the mycelium to grow thick, but slow. It is a good idea to add yeast and peptone, as it adds some more nutrients to promote healthy growth...
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To give is to live...
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YSr
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Re: Oatmeal agar - 2 questions [Re: ATWAR]
#3303914 - 11/01/04 06:29 AM (7 years, 3 months ago) |
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Both yeast and peptone? 2 gram each?
YSr
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ragadinks
MrBeatle
Registered: 10/20/03
Posts: 1,296
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Re: Oatmeal agar - 2 questions [Re: YSr]
#3304532 - 11/01/04 11:01 AM (7 years, 3 months ago) |
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The oatmeal recipes in FastFred's Media Cookbook do not use peptone or dextrose at all ...
Edit: Also Una's oatmeal recipe does not use dextrose and peptone.
Edited by ragadinks (11/01/04 11:07 AM)
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ATWAR
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Re: Oatmeal agar - 2 questions [Re: YSr]
#3307594 - 11/01/04 11:28 PM (7 years, 3 months ago) |
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Quote:
YSr said:
Both yeast and peptone? 2 gram each?
YSr
For a liter of agar I would add 1 gram of peptone and 2 grams of nutritional yeast. There are recipes that have yeast and peptone, and those without. This does not mean that yeast or peptone cannot (or should not) be added as a supplement. It does indeed promote healthy growth...
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To give is to live...
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ragadinks
MrBeatle
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Re: Oatmeal agar - 2 questions [Re: ATWAR]
#3317876 - 11/03/04 10:20 PM (7 years, 3 months ago) |
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Anyone ever tried oatmeal agar ?
And if yes: how is this kind of agar compared to normal PDYA ?
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ATWAR
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Re: Oatmeal agar - 2 questions [Re: ragadinks]
#3324338 - 11/05/04 11:04 AM (7 years, 3 months ago) |
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I have not run any direct comparisons between the different medias (the common ones that is). OMYA has performed fine for me the very few times I have used it. It has done the job, and done it seemingly just as well as MYPA. I suggest you grind the oatmeal up in a mortar and pestle or coffee grinder and do not strain it from the brew...
Other than that, it is just like PDA in regards to the fact that it requires more preparation than I would like to perform (boil it a bit to soften the oatmeal and extract the goodies, then PC as normal). MEA and its supplemented cousins are so much easier for the lazy person like me...
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To give is to live...
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