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 @cro
new name
Registered: 12/07/02
Posts: 1,223
Loc: The PNW
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 Re: multispore agar innoculation [Re: Scourge]
#2407101 - 03/08/04 12:52 PM (4 years, 8 months ago) |
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Yeah, peroxide's good at killing all types of spores, including cube spores. If it is a multispore inocculation peroxidated agar won't let anything grow. Although, if in the slightest of concentrations it could work and has I believe Ganja/Paid/Loki tried this and it worked for him.
Pea ce - @cro
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 RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 18,191
Loc: USA Mountain Northwest
Last seen: 8 hours, 53 minutes
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Re: multispore agar innoculation [Re: mycohomme]
#2411739 - 03/09/04 05:35 PM (4 years, 8 months ago) |
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Another good trick is to cool the inoculating loop in the agar of the receiving dish. This will leave a trace amount of agar on the loop and help when you use it to collect spores. Just touch it lightly to the print and thousands of spores will stick to it. Personally, I try to bury the spores in the petri dish by rotating the loop into the agar in one spot in the very center of the dish. I disagree with spreading or streaking the spores around the dish. Try to keep them all in the center, so after germination they can do that 'mushroom sex' thing easily, then sector into the various strains you would isolate.
Perhaps what blue helix is saying is he's never seen a 'multispore' created project refuse to fruit unless contaminated. I have, but that's not the issue. I always isolate until there are no more sectors, then fruit(or try to) each substrain. By keeping masters, I can determine the best fruiting isolate, then go back to the refrigerator and only grow out that one for future cycles. I've done this with every strain in my library. If I isolate ten substrains(sectors) from a petri dish, one might fruit very well, two might fruit so-so the way multispore would, and it is very common that the other seven won't ever set a pin.
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 18,191
Loc: USA Mountain Northwest
Last seen: 8 hours, 53 minutes
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Re: multispore agar innoculation [Re: RogerRabbit]
#2411747 - 03/09/04 05:39 PM (4 years, 8 months ago) |
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Also,
When using a spore syringe with agar, simply use one drop in the very center of the dish. Try to keep the dish flat when you wrap the parafilm around it to keep that drop right in the center. It will work better for you that way.
--------------------
www.mushroomvideos.com
"I wouldn't want to belong to any club that would have me as a member".
Mark Twain, Woody Allen, Groucho Marx, and anyone else who wishes to claim credit for the quote.
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