Home | Community | Message Board


Visionary TeesPlease support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation >> Mushroom Cultivation Archive >> Agar and Liquid Culture

Welcome to the Shroomery Message Board! Please login or register to post messages and view our members-only content. You'll gain access to additional forums, encrypted messages, file attachments, board customizations, and much more!

Shop for:   eBay Pressure Cooker, 10cc Syringe   Amazon ½ Pint Jars

Pages: 1 | 2 | 3 | 4  [ show all ]
Offlineangryjslice
now with 20%more anger
Male

Folding@home Statistics
Registered: 10/29/03
Posts: 916
Last seen: 1 day, 19 hours
Super easy karo tek
    #2321056 - 02/10/04 12:13 PM (4 years, 10 months ago)

Do you have a microwave? Tired of messing up w/ your karo teks?

heres your solution:

supplies your gonna need:

1 package of small microwavable containers - like these:



1 bottle cheap imitation light syrup - like this:



1 package cheap plastic wrap - like this:



total cost should be under $5

ok now heres what you do:

1) pour about 200ml's of water (around 5 ounces) into the container.

2) add a teaspoon of light syrup to the water(im sure more could be used to increase results, but this is just what i did)

3) put a peice of plastic wrap over the top of the container, and then put the lid on top of that - like this:



4) microwave the container, with the lid slightly cracked (so the container doesnt melt) for 5 Minutes . when its done nuking, open your microwave and push the lid down so its all sealed in there (carefull, its hot)

5) let cool for a few hours, or untill it is room temperature again, and then lift up the lid slowly, flame sterilize your syringe, and put in 1 - 2cc's of your left over spore solution.

6) toss these guys in your incubator (im using a double tub setup)



7) after a few days you should start to see some myc. growth in the solution. after about a week and a half your container should look like this.



i shake the containers around every few days and let the water sloshing break up the myc. no need for glass chunks.

when your ready to innoculate, just lift up the lid, and poke the needle of your sterile syringe through the plastic wrap and suck up how much ever juice you need.

congratulations...you just spent $5 to turn 2 cc's of spore solution into 200 cc's of spore solution. easy as pie in a microwave too.


well i hope this helps someone. enjoy everybody.

~JSlice~


--------------------


Post Extras: Print Post  Remind Me!  Notify Moderator   Ignore User 
OfflineSillllyFressshhhh
newbie
Registered: 01/24/04
Posts: 33
Last seen: 4 years, 9 months
Re: Super easy karo tek [Re: angryjslice]
    #2321080 - 02/10/04 12:22 PM (4 years, 10 months ago)

WOW Sounds fantastic I think Ima try this tonight once I get home from work, THANKS!


Post Extras: Print Post  Remind Me!  Notify Moderator   Ignore User 
Offlineniteowl
GrandPaw
Male User Gallery

Registered: 07/01/03
Posts: 7,255
Loc: Arkansas
Last seen: 6 hours, 40 minutes
Re: Super easy karo tek [Re: angryjslice]
    #2321129 - 02/10/04 12:40 PM (4 years, 10 months ago)

I have done the same thing with half pint jars. Wrap the plastic over the top and nuke. When done nuking put the lids w/hole on, and incubate.
When they are ready just poke the needle thru the hole and suck up.


--------------------
Being happy doesn't mean everything is perfect.
It means you've decided to see beyond the imperfections

"Fear is the mind killer.
I will face my fear and let it pass thru me"
Paul Atreides: Dune


Post Extras: Print Post  Remind Me!  Notify Moderator   Ignore User 
OfflineBen_Stein
Spelling Gestapo

Registered: 08/15/03
Posts: 370
Loc: Hooterville
Last seen: 4 years, 4 months
Re: Super easy karo tek [Re: SillllyFressshhhh]
    #2321234 - 02/10/04 01:14 PM (4 years, 10 months ago)

Quote:

SillllyFressshhhh said:
WOW Sounds fantastic I think Ima try this tonight once I get home from work, THANKS!




Shit! I'm gonna quit my job, go home and try it...NOW. :lol:

Nice tip, jslice...tanks :thumbup:

I would consider using two teaspoons of syrup to 200ml, ,though, but it shonuf worked.


--------------------
I'm so ready to start growing. I've shroomed twice and loved it to no extent. --Mindgames


Post Extras: Print Post  Remind Me!  Notify Moderator   Ignore User 
OfflineGr0wer
cultivator &mycologist

Registered: 09/16/03
Posts: 2,423
Last seen: 1 year, 9 months
Re: Super easy karo tek [Re: angryjslice]
    #2321418 - 02/10/04 02:03 PM (4 years, 10 months ago)

Is that last pic what a fully colonized solution looks like?


Post Extras: Print Post  Remind Me!  Notify Moderator   Ignore User 
Offlineangryjslice
now with 20%more anger
Male

Folding@home Statistics
Registered: 10/29/03
Posts: 916
Last seen: 1 day, 19 hours
Re: Super easy karo tek [Re: Gr0wer]
    #2321681 - 02/10/04 03:21 PM (4 years, 10 months ago)

that last picture is when ive taken it out of the incubator at this point...its colonized enough to the point that i can make 20 syringes out of it.

~JSlice~


--------------------


Post Extras: Print Post  Remind Me!  Notify Moderator   Ignore User 
OfflineMagicBrownie
Shroomacologist
Registered: 01/30/04
Posts: 39
Last seen: 4 years, 2 months
Re: Super easy karo tek [Re: angryjslice]
    #2322055 - 02/10/04 08:38 PM (4 years, 10 months ago)

Why does this seem too good to be true?

Does this really work?

Is light syrup the same as corn syrup?

Is it actually sucking spores into the syringe, or mycelium?


--------------------
"From one shroom comes a million."
The Shroomacologist


Post Extras: Print Post  Remind Me!  Notify Moderator   Ignore User 
OfflineYarry
Ween
Male User Gallery

Registered: 01/04/04
Posts: 23,538
Last seen: 7 days, 11 hours
Re: Super easy karo tek [Re: MagicBrownie]
    #2322073 - 02/10/04 08:42 PM (4 years, 10 months ago)

Yeah it works, i did the same with a little molasses. and itll be myc in your syringe not spores. so not legal to trade.. jus wanna make sure everyone knows that. dont want to get yourself in trouble


--------------------
CannabisCharlie is my apprentice.


Post Extras: Print Post  Remind Me!  Notify Moderator   Ignore User 
OfflineMagicBrownie
Shroomacologist
Registered: 01/30/04
Posts: 39
Last seen: 4 years, 2 months
Re: Super easy karo tek [Re: Yarry]
    #2322091 - 02/10/04 08:44 PM (4 years, 10 months ago)

How long can i wait to inoculate a jar with the syringe?

Will it be just as fast and effective as spores?


--------------------
"From one shroom comes a million."
The Shroomacologist


Post Extras: Print Post  Remind Me!  Notify Moderator   Ignore User 
OfflineBen_Stein
Spelling Gestapo

Registered: 08/15/03
Posts: 370
Loc: Hooterville
Last seen: 4 years, 4 months
Re: Super easy karo tek [Re: MagicBrownie]
    #2322118 - 02/10/04 08:48 PM (4 years, 10 months ago)

It's lots faster cuz the spores are already germinated, cutting out at least five days.

Also, it seems to grow faster in general, plus you no longer have to be stingy with your apores.


--------------------
I'm so ready to start growing. I've shroomed twice and loved it to no extent. --Mindgames


Post Extras: Print Post  Remind Me!  Notify Moderator   Ignore User 
OfflineMagicBrownie
Shroomacologist
Registered: 01/30/04
Posts: 39
Last seen: 4 years, 2 months
Re: Super easy karo tek [Re: Ben_Stein]
    #2322131 - 02/10/04 08:51 PM (4 years, 10 months ago)

This is the best news ive heard in a long time!

i only have 3 cc's of solution left in my syringe, and i cant wait to give this a try!


--------------------
"From one shroom comes a million."
The Shroomacologist


Post Extras: Print Post  Remind Me!  Notify Moderator   Ignore User 
Offlineangryjslice
now with 20%more anger
Male

Folding@home Statistics
Registered: 10/29/03
Posts: 916
Last seen: 1 day, 19 hours
Re: Super easy karo tek [Re: MagicBrownie]
    #2322213 - 02/10/04 09:05 PM (4 years, 10 months ago)

using liquid culture ive colonized half pints completly in 5 days. this isnt anything new that hasnt been done before, but i havnt ever seen a pictorial or instructions for karo in the microwave.

~JSlice~


--------------------


Post Extras: Print Post  Remind Me!  Notify Moderator   Ignore User 
OfflineGr0wer
cultivator &mycologist

Registered: 09/16/03
Posts: 2,423
Last seen: 1 year, 9 months
Re: Super easy karo tek [Re: Yarry]
    #2322281 - 02/10/04 09:16 PM (4 years, 10 months ago)

Quote:

YarryShroomer said:
Yeah it works, i did the same with a little molasses.




How well did the molasses work? Was it normal dark molasses?


Post Extras: Print Post  Remind Me!  Notify Moderator   Ignore User 
OfflineYarry
Ween
Male User Gallery

Registered: 01/04/04
Posts: 23,538
Last seen: 7 days, 11 hours
Re: Super easy karo tek [Re: Gr0wer]
    #2322301 - 02/10/04 09:19 PM (4 years, 10 months ago)

Yeah normal dark molasses, it worked pretty well, same amount of myc just a little harder to see cuz its darker. i didnt really do any measurements, just filled a half pint jar with water and added molasses til it looked slightly darker then whiskey. tossed on saran wrap and a plastic lid and microwaved er


--------------------
CannabisCharlie is my apprentice.


Post Extras: Print Post  Remind Me!  Notify Moderator   Ignore User 
OfflineNickster_154371
Hobbyist
Male
Registered: 01/01/04
Posts: 432
Loc: where I believe I belong
Last seen: 11 hours, 8 minutes
Re: Super easy karo tek [Re: Yarry]
    #2335240 - 02/14/04 06:01 PM (4 years, 10 months ago)

When your incubating the karo container do you need to have holes punched in it for gas exchange?


Post Extras: Print Post  Remind Me!  Notify Moderator   Ignore User 
Offlinesimplemachine
mushroom disposal unit
Male

Registered: 09/14/03
Posts: 1,887
Loc: high and dry
Last seen: 3 days, 14 hours
Re: Super easy karo tek [Re: Nickster_154371]
    #2335288 - 02/14/04 06:18 PM (4 years, 10 months ago)

^^^no it should be as well sealed as possible.


SM


--------------------


Post Extras: Print Post  Remind Me!  Notify Moderator   Ignore User 
OfflineBen_Stein
Spelling Gestapo

Registered: 08/15/03
Posts: 370
Loc: Hooterville
Last seen: 4 years, 4 months
Re: Super easy karo tek [Re: simplemachine]
    #2335562 - 02/14/04 08:09 PM (4 years, 10 months ago)

^^^ yeah, sealed tight. The culture will live for a few months off the air already in there, which is really nice.


--------------------
I'm so ready to start growing. I've shroomed twice and loved it to no extent. --Mindgames


Post Extras: Print Post  Remind Me!  Notify Moderator   Ignore User 
Offlinegugglebum
newbie
Registered: 01/02/04
Posts: 35
Last seen: 4 years, 3 months
Re: Super easy karo tek [Re: Ben_Stein]
    #2336714 - 02/15/04 08:15 AM (4 years, 10 months ago)

Aren't the Shroomery sponsors going to be really pissed off at whoever created the Karo Tek? Oh well ... hehehe


Post Extras: Print Post  Remind Me!  Notify Moderator   Ignore User 
OfflineBen_Stein
Spelling Gestapo

Registered: 08/15/03
Posts: 370
Loc: Hooterville
Last seen: 4 years, 4 months
Re: Super easy karo tek [Re: gugglebum]
    #2336772 - 02/15/04 08:57 AM (4 years, 10 months ago)

Quote:

gugglebum said:
Aren't the Shroomery sponsors going to be really pissed off at whoever created the Karo Tek? Oh well ... hehehe




The tek prolly has been around longer than most of them.

Unfortunately, it's the nature of the business (the spore part, anyway) that you may or may not see a lot of repeat business.

Happily, most of the sponsors seem to be holding their own.


--------------------
I'm so ready to start growing. I've shroomed twice and loved it to no extent. --Mindgames


Post Extras: Print Post  Remind Me!  Notify Moderator   Ignore User 
OfflineBruce3

Registered: 02/08/04
Posts: 86
Loc: some where over the rainb...
Last seen: 7 months, 24 days
Re: Super easy karo tek [Re: angryjslice]
    #2337017 - 02/15/04 11:08 AM (4 years, 10 months ago)

do you poke through the plastic wrap when injecting the spores? or do you lift it up?


Post Extras: Print Post  Remind Me!  Notify Moderator   Ignore User 
OfflineAkrobatik
Chillin

Registered: 01/16/04
Posts: 267
Last seen: 1 year, 9 months
Re: Super easy karo tek [Re: Bruce3]
    #2337049 - 02/15/04 11:21 AM (4 years, 10 months ago)

poke def, you dont want to expose the myc to any air unless you have a glove box or somthing similar.
:mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2:


--------------------
:mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2:


Post Extras: Print Post  Remind Me!  Notify Moderator   Ignore User 
InvisiblePsychoslut
 User Gallery

Registered: 12/10/02
Posts: 15,296
Loc: all up in ya
Re: Super easy karo tek [Re: angryjslice]
    #2337737 - 02/15/04 02:38 PM (4 years, 10 months ago)

great, but i think your thinking a little too far into it, it does not need to be this complicated, with all the surran wrap and junk, just have the lid on with an innoc hole, see the steam in the container is going to cause a positive pressure environment in the container, not allowing dirty air to enter the hole, long enough for you to throw on a piece of tape before it cools, then just innoc like ya would with a half pint when its cooled, really all this tek did is eliminate the metal lid on a jar, im not bashing you at all though, its cool.

4) microwave the container, with the lid slightly cracked(so the container doesnt melt) for 5 Minutes . when its done nuking, open your microwave and push the lid down so its all sealed in there (carefull, its hot)

it wont melt, all of the plastic storage containers are microwave and freezer safe these days, you just need a vent for steam so the lid doesnt pop off, wich is why you just make your innoc hole in the lid before microwaving, sterilize it, take it out of the nuker, and throw on some tape over the hole asap.


--------------------
I am pro gun control. I believe everyone should be in control of a gun.

Edited by Psychoslut (02/15/04 02:59 PM)


Post Extras: Print Post  Remind Me!  Notify Moderator   Ignore User 
OfflineNickster_154371
Hobbyist
Male
Registered: 01/01/04
Posts: 432
Loc: where I believe I belong
Last seen: 11 hours, 8 minutes
Re: Super easy karo tek [Re: Bruce3]
    #2338183 - 02/15/04 04:40 PM (4 years, 10 months ago)

poke through


Post Extras: Print Post  Remind Me!  Notify Moderator   Ignore User 
OfflineTinTree
thread killer(semi pro)
Male User Gallery

Registered: 02/15/04
Posts: 1,456
Loc: Mu
Last seen: 7 months, 8 days
Re: Super easy karo tek [Re: Nickster_154371]
    #2338287 - 02/15/04 05:15 PM (4 years, 10 months ago)

Somebody asked earlier, and I'm curious too.
How long can you store the fluid before inoculation?
(First post here btw... hi everybody!)


--------------------
"I'm afraid of losing my obscurity. Genuineness only thrives in the dark. Like celery."
- Aldous Huxley


Post Extras: Print Post  Remind Me!  Notify Moderator   Ignore User 
Offlinesimplemachine
mushroom disposal unit
Male

Registered: 09/14/03
Posts: 1,887
Loc: high and dry
Last seen: 3 days, 14 hours
Re: Super easy karo tek [Re: TinTree]
    #2338325 - 02/15/04 05:29 PM (4 years, 10 months ago)

I've stored LC jars for weeks before innoculating.


SM


--------------------


Post Extras: Print Post  Remind Me!  Notify Moderator   Ignore User 
Offlinesimplemachine
mushroom disposal unit
Male

Registered: 09/14/03
Posts: 1,887
Loc: high and dry
Last seen: 3 days, 14 hours
Re: Super easy karo tek [Re: angryjslice]
    #2338344 - 02/15/04 05:37 PM (4 years, 10 months ago)

nice work j...

this might make liquid culture a bit more n00b friendly. The only thing I would suggest is the addition of some peroxide.

AFTER the spores have germinated several cc's of peroxide (about 5 or so for that much water) should be addded through the innoculation hole. The peroxide prevents contams and speeds up colonization by adding 02 to the karo solution.


sM


--------------------


Post Extras: Print Post  Remind Me!  Notify Moderator   Ignore User 
Offlinevermonster
Lost Sailor

Registered: 10/17/03
Posts: 205
Loc: Shangri La
Last seen: 2 months, 12 days
Re: Super easy karo tek [Re: simplemachine]
    #2338587 - 02/15/04 07:10 PM (4 years, 10 months ago)

Afoaf has used a 1/2 pt jar with a plastic lid for the same
idea. She has to heat it for 1 min and then let it cool for
a bit and heat it again. If she does it for 5 min straight
the innoc hole is too small too relieve excess pressure and
the top pops off. She also covers it with a coffee filter for
heating and then tyvek for incubati