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OfflineRastafari
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Registered: 01/29/03
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Questions about Reishi & Reishi-Mycelium *Extracts*
    #2191885 - 12/19/03 09:58 PM (20 years, 3 months ago)

Dear Shroomerites,

I was wondering if anyone knew about extraction of reishi mycelium and/or reishi fruits

I hear the mycelium is more potent, and its easier to extract...

What would be the best (easiest) method to extract with (alcohol?)

What would be the best substrate to grow JUST mycelium on? for extraction and/or dried mycelium.

do you know which stages the mycelium is most potent?

Thanks!


--------------------
I&I

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Offlineragadinks
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Re: Questions about Reishi & Reishi-Mycelium *Extracts* [Re: Rastafari]
    #2192245 - 12/20/03 01:52 AM (20 years, 3 months ago)

Quote:

What would be the best substrate to grow JUST mycelium on? for extraction and/or dried mycelium.




think liquid media would be the easiest/fastest way to grow only mycelium. But I do not know it this mycelium would still have same amount of alkaloids etc.

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OfflineThai_connection
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Re: Questions about Reishi & Reishi-Mycelium *Extracts* [Re: ragadinks]
    #4993487 - 11/29/05 10:03 AM (18 years, 4 months ago)

dry mycelium can be done either by agar or liquid culture, liquid is faster and easier and less messy but doesn't get the same volume as agar imo. for agar add some woodchips pour it in a bottle for more mass, one can try to rotate the bottle while it cools to get a whole inside surface coated with agar but you will find out that it is not as easy as said as the agar is very fragile and slides..with gravity pulls you most likely end up in a mush of agar. just remember the more mass you have to colonize, will also take more time... it is a race against contams and most of us do not have a lab... so keep it simple.
after the agar is colonized just pull the mycelium off.. like peeling bananas.rinse, dry,

for liquid cultures,this is when magnectic stiring comes in great use. when thick with mycelium..drain into a cheese cloth. rinse if desire, dry and storage.

peace
siam

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Invisiblemushrx1
rainy season
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Registered: 04/22/05
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Re: Questions about Reishi & Reishi-Mycelium *Extracts* [Re: Rastafari]
    #5000914 - 11/30/05 11:21 PM (18 years, 3 months ago)

Hey Rastafari,
Christopher Hobbs says that extracts are okay, but for homemade you should stick to boiling fruit bodies for tea. I use a coffee grinder to pulverize the sliced conks.

Mycelia are _not as strong in beta 1,3 glucans, as these are found in highest concentration in the cap tissue (mantle) according to some Japanese researchers. (also cited in C. Hobbs). Mycelia are favored for production, since it is faster and therefore cheaper to produce.

As for producing mycelia, though, liquid culture is the best route. Those that produce grain mycelia generally powder the digested grain for capsules.

Reishi extracts can refer to hot water extracts, where the liquid used is evaporated to produce a concentrated powderable substance. Some capsules include this type of extract. Beta 1,3 glucans are water soluble and not subject to vaporization at normal boiling temps (again, according to C. Hobbs.)

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Offlinecurenado
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Re: Questions about Reishi & Reishi-Mycelium *Extracts* [Re: mushrx1]
    #5058594 - 12/13/05 05:07 PM (18 years, 3 months ago)

Rastafari ~
"Full Spectrum" extraction is achieved by alcohol immersion for 1 week (stirring/mashing daily) and then placing the squeezed out mushroom pulp in a pot of water and boiling at a moderate boil for 30 minutes.
The water pushes out the remaining alcohol and it's constituents.
Now combine the water and alcohol extracts and reduce over LOW heat to evaporate off most of the alcohol and concentrate the extract.
Voila!~
I still prefer to recommend the whole fruit, but do make extracts here and there.
to make a dose of one ounce three times per day, try using about 1/3 cup ground mushroom for 1 quart h2o.
The same extraction for mycelium would apply, but the above formula geared for whole fruit.
Best Wishes!  :smile:


--------------------
Yours in the Natural State!
"The woods are lovely, dark and deep; but I have patches to keep, and jars to sterilize before I sleep...."

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InvisibleBsdgaou

Registered: 02/20/19
Posts: 340
Re: Questions about Reishi & Reishi-Mycelium *Extracts* [Re: curenado]
    #26723208 - 06/06/20 01:21 AM (3 years, 9 months ago)

Quote:

curenado said:
Rastafari ~
"Full Spectrum" extraction is achieved by alcohol immersion for 1 week (stirring/mashing daily) and then placing the squeezed out mushroom pulp in a pot of water and boiling at a moderate boil for 30 minutes.
The water pushes out the remaining alcohol and it's constituents.
Now combine the water and alcohol extracts and reduce over LOW heat to evaporate off most of the alcohol and concentrate the extract.
Voila!~
I still prefer to recommend the whole fruit, but do make extracts here and there.
to make a dose of one ounce three times per day, try using about 1/3 cup ground mushroom for 1 quart h2o.
The same extraction for mycelium would apply, but the above formula geared for whole fruit.
Best Wishes!  :smile:




I know it's old thread...
But can you evaporate water too? So you get "extra potency" in small bottle.

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OfflineForresterM
aspiring sociopath
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Registered: 02/05/13
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Re: Questions about Reishi & Reishi-Mycelium *Extracts* [Re: Bsdgaou]
    #26723425 - 06/06/20 05:54 AM (3 years, 9 months ago)

Quote:

Bsdgaou said:
But can you evaporate water too? So you get "extra potency" in small bottle.




Short answer - yes.

Just don't evaporate too much water, or the water solubles will come out of solution. 

If you've let your extraction sit long enough for all the mushroom "dust" to settle out, and removed that, you can tell if something is precipitating out of solution as you will see it on the bottom of the jar.


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.

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InvisibleBsdgaou

Registered: 02/20/19
Posts: 340
Re: Questions about Reishi & Reishi-Mycelium *Extracts* [Re: Forrester]
    #26723587 - 06/06/20 08:27 AM (3 years, 9 months ago)

Quote:

Forrester said:
Quote:

Bsdgaou said:
But can you evaporate water too? So you get "extra potency" in small bottle.




Short answer - yes.

Just don't evaporate too much water, or the water solubles will come out of solution. 

If you've let your extraction sit long enough for all the mushroom "dust" to settle out, and removed that, you can tell if something is precipitating out of solution as you will see it on the bottom of the jar.




How do you mean "out of solution"? Even if I evaporate all of water (which I won't), it should be some "dust" at the bottom, so ingredients we want in our tinctures. Or?

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OfflineForresterM
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Re: Questions about Reishi & Reishi-Mycelium *Extracts* [Re: Bsdgaou]
    #26724069 - 06/06/20 12:35 PM (3 years, 9 months ago)

Well sure, if you want some or most of your good medicine as a dust (or more likely, tar) in the bottom of your liquid, you can do that.  But then you're not likely to have it evenly distributed into your tincture, and will end up getting either none or all of it it one dose.  Know what I mean?

Because the alcohol in a double extraction will evaporate off first, at some point your alcohol solubles won't have anything to be dissolved in, and will come out of solution.  Ever seen salt water evaporate?  And all the dissolved salt starts turning to powder again?  That's what I mean by out of solution.


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.

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